本文選題：暈船　切入點：蛋白質組　出處：《第二軍醫(yī)大學學報》2005年04期 　論文類型：期刊論文

【摘要】：目的:利用蛋白組學技術,建立暈船易感及不暈大鼠腦干總蛋白的雙向電泳圖譜,分析并鑒定差異表達蛋白質。方法:據模擬暈船刺激后異嗜高嶺土量的增減將大鼠分為暈船易感組和不暈組,應用固相pH梯度(immobilized pH gradient,IPG)雙向電泳(two dimensional electrophoresis,2 DE)技術,分離2 組大鼠腦干總蛋白,分析并用肽指紋圖譜(peptide massfingerprint,PMF)鑒定差異蛋白質。結果:鑒定5個暈船易感相關蛋白質,上調酪氨酸3 單加氧酶/色氨酸5 單加氧酶激活蛋白、鐵效應元件結合蛋白1,下調硫氧還蛋白過氧化物酶Ⅰ、磷酸甘油酸變位
[Abstract]:Objective: to establish a two-dimensional electrophoretic map of brain stem total protein in seasickness susceptible and non-dizzy rats by proteomics technique. Methods: according to the increase and decrease of heterophilic kaolin content after simulated seasickness stimulation, rats were divided into seasickness susceptible group and non halo group. Brain stem total proteins were isolated from two groups of rats and identified by peptide fingerprint peptide fingerprinting PMF. Results: five seasickness related proteins were identified, and tyrosine 3 monooxygenase / tryptophan 5 monooxygenase activator protein were upregulated. Iron effector element binding protein 1, down-regulating thioredoxin peroxidase 鈪，

本文編號：1605971