本文選題：富氫水　切入點(diǎn)：熒光定量PCR　出處：《食品科技》2017年05期 　論文類型：期刊論文

【摘要】：通過(guò)研究富氫水(Hydrogen-rich water)對(duì)Wistar雄性大鼠腸道菌群的影響,建立了富氫水和腸道微生態(tài)之間的關(guān)聯(lián),并研究了富氫水對(duì)模擬失重大鼠的腸道微生態(tài)調(diào)節(jié)作用,為預(yù)防航天員腸道微生態(tài)的失調(diào)和促進(jìn)飛行后腸道微生態(tài)平衡的恢復(fù)提供依據(jù)。研究通過(guò)構(gòu)建含有待測(cè)細(xì)菌的16S rRNA基因序列的重組質(zhì)粒作為標(biāo)準(zhǔn)品,利用SYBR GreenⅠ實(shí)時(shí)熒光定量PCR的方法,檢測(cè)富氫水對(duì)大鼠盲腸和結(jié)腸內(nèi)容物中5種常見(jiàn)細(xì)菌的影響。試驗(yàn)成功構(gòu)建了5種腸道菌的標(biāo)準(zhǔn)曲線,并實(shí)現(xiàn)了對(duì)腸內(nèi)容物中5種細(xì)菌的定量。結(jié)果顯示,模擬失重使腸道中腸球菌和大腸桿菌的拷貝數(shù)上升,產(chǎn)氣莢膜梭菌的拷貝數(shù)下降,而富氫水對(duì)腸球菌則有明顯的抑制作用。
[Abstract]:By studying the effect of Hydrogen-rich water on intestinal flora of Wistar male rats, the relationship between hydrogen rich water and intestinal microecology was established, and the effects of hydrogen rich water on intestinal microecology of simulated weightlessness rats were studied. In order to prevent the disturbance of intestinal microecology in astronauts and to promote the restoration of intestinal microecological balance after flight, the recombinant plasmid containing 16s rRNA gene sequence of bacteria to be tested was constructed as the standard. The effect of hydrogen-rich water on five common bacteria in the caecum and colon of rats was detected by SYBR Green 鈪，

本文編號(hào)：1579723