本文關(guān)鍵詞： 重力變化 神經(jīng)母細(xì)胞瘤 氧化應(yīng)激　出處：《生理學(xué)報(bào)》2009年05期 　論文類(lèi)型：期刊論文

【摘要】：本研究通過(guò)對(duì)與氧化還原狀態(tài)相關(guān)指標(biāo)的免疫細(xì)胞化學(xué)分析,研究重力變化條件下細(xì)胞的氧化應(yīng)激狀態(tài);探討細(xì)胞氧化還原關(guān)鍵分子硫氧還蛋白(thioredoxin,TRX)對(duì)重力變化條件下細(xì)胞氧化還原狀態(tài)的影響。采用神經(jīng)母細(xì)胞瘤細(xì)胞(SH-SY5Y細(xì)胞)作為研究對(duì)象,分為4組:地面對(duì)照組、轉(zhuǎn)染TRX細(xì)胞地面對(duì)照組、飛行組、轉(zhuǎn)染TRX細(xì)胞飛行組,以免疫細(xì)胞化學(xué)方法觀察拋物線(xiàn)飛行條件下,SH-SY5Y細(xì)胞硝基酪氨酸(3-nitrotyrosine,3-NT)、誘導(dǎo)型一氧化氮合酶(inducible nitric oxide synthase,iNOS)、TRX以及硫氧還蛋白還原酶(thioredoxin reductase,TRXR)表達(dá)的變化。結(jié)果顯示,與地面對(duì)照組相比,飛行組拋物線(xiàn)飛行后,3-NT表達(dá)水平升高,TRX染色強(qiáng)度略增強(qiáng),TRXR表達(dá)下調(diào);與飛行組相比,轉(zhuǎn)染TRX細(xì)胞飛行組iNOS表達(dá)下調(diào),3-NT水平降低。上述結(jié)果表明,重力變化可以誘導(dǎo)iNOS、3-NT、TRX的表達(dá),下調(diào)TRXR的表達(dá),增加SH-SY5Y細(xì)胞的氧化應(yīng)激水平,TRX轉(zhuǎn)染細(xì)胞能在一定程度上對(duì)抗重力變化引起的氧化應(yīng)激。
[Abstract]:In this study, we studied the oxidative stress state of the cells under the condition of gravity change by immunocytochemical analysis of the indexes related to the redox state. To investigate the effect of thioredoxin (TRX), a key molecule of cell redox, on the redox state of cells under the condition of gravity change, the neuroblastoma cell line SH-SY5Y was used as the study object, which was divided into four groups: ground control group. TRX cells were transfected into ground control group, flight group and TRX cell flight group. The expression of nitrotyrosine 3-nitrotyrosine, inducible nitric oxide synthase and thioredoxin reductase TRXRX in SH-SY5Y cells under parabolic flight was observed by immunocytochemistry. The results showed that the expression of nitrotyrosine, inducible nitric oxide synthase and thioredoxin reductase TRXRX in SH-SY5Y cells were higher than those in the control group. In flight group, the expression of iNOS in flight group was down-regulated, and the expression of iNOS in flight group was lower than that in flight group. The above results indicated that the changes of gravity could induce the expression of iNOS3-NTTX in flight group, and the expression of iNOS in flight group was lower than that in flight group, the results indicated that the change of gravity could induce the expression of iNOS3 NTTRX in flight group. The expression of TRXR was down-regulated and the oxidative stress level of SH-SY5Y cells was increased. TRX-transfected cells could antagonize the oxidative stress induced by gravity changes to some extent.
【作者單位】： 中國(guó)航天員科研訓(xùn)練中心;
【基金】：supported by the National Natural Science Foundation of China(No.30570452,30600759)
【分類(lèi)號(hào)】：R855

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本文編號(hào)：1505102