本文關(guān)鍵詞：高原兔肝臟火器傷后機(jī)體應(yīng)激性變化的研究　出處：《石河子大學(xué)》2011年碩士論文　論文類(lèi)型：學(xué)位論文

  更多相關(guān)文章： GR 氧化應(yīng)激 應(yīng)激 高原 肝臟 火器傷

【摘要】：目的：探討兔在高原環(huán)境下肝臟火器傷后血清皮質(zhì)醇(Cor)、促腎上腺皮質(zhì)激素(ACTH)、丙二醛(MDA)、超氧化物歧化酶(SOD)、全血谷胱甘肽(GSH)及肺組織糖皮質(zhì)激素受體(GR)的動(dòng)態(tài)變化規(guī)律及其意義,旨在提高高原肝外傷的綜合救治水平,降低肝外傷的死亡率,同時(shí)也為防治高原肝臟火器傷后機(jī)體多器官功能障礙綜合癥提供理論依據(jù)。 方法:采用成年健康新西蘭大白兔144只,隨機(jī)分為平原肝臟火器傷組(A組)、初入高原肝臟火器傷組(B組)、適應(yīng)高原肝臟火器傷組(C組),各組又細(xì)分為正常對(duì)照組和傷后0.5、2、6、12、24、48、72h,每組6只。在不同海拔環(huán)境下成功建立肝臟火器傷模型后,分別于各時(shí)間點(diǎn)采集血液標(biāo)本和左下肺組織,采用固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)測(cè)定各組血清Cor和ACTH;硫代巴比妥酸法測(cè)定各組血清MDA含量；黃嘌呤氧化酶法測(cè)定各組血清SOD活力；比色定量法測(cè)定各組全血GSH含量；免疫組織化學(xué)圖像分析法測(cè)定各組肺組織GR的表達(dá)。 結(jié)果:①與正常組比較,肝臟火器傷后3組兔血清Cor、ACTH含量持續(xù)升高,6h達(dá)峰值(P0.01),12h仍處于較高水平,之后進(jìn)行性下降；傷后MDA含量持續(xù)升高,12h達(dá)峰值(P0.01),繼而進(jìn)行性下降,72h仍高于正常,而SOD活力、GSH含量早期略有升高,以后逐漸下降,到達(dá)最低值后逐漸恢復(fù)；傷后GR表達(dá)開(kāi)始下降,最低點(diǎn)出現(xiàn)在6h,之后緩慢恢復(fù),72h仍未恢復(fù)至正常水平。②與平原火器傷組比較,初入高原正常組Cor、ACTH含量已高于平原正常組(P0.01),致傷后升高幅度及最高值水平也更高；MDA到達(dá)峰值水平(P0.01)和SOD活力、GSH含量早期升高及以后到達(dá)最低值水平更顯著；GR表達(dá)致傷后下降幅度及最低值水平也更顯著(P0.01)。③與平原火器傷組比較,適應(yīng)高原各組Cor、ACTH含量升高幅度及到達(dá)最高值水平；MDA升高幅度和SOD活力、GSH含量降低幅度(P0.05);GR表達(dá)下降幅度及最低值水平都更顯著,但其顯著程度又低于初入高原組。 結(jié)論:①高原肝臟火器傷后,在創(chuàng)傷和高原低氧雙重應(yīng)激的影響下,機(jī)體產(chǎn)生了明顯的應(yīng)激反應(yīng),其程度明顯強(qiáng)于平原創(chuàng)傷應(yīng)激；②火器傷打破了體內(nèi)氧化與抗氧化之間的平衡,同時(shí),高原低氧可使機(jī)體產(chǎn)生過(guò)多的自由基,并且使機(jī)體清除自由基的能力下降,氧自由基可能參與火器傷后機(jī)體的病理生理過(guò)程；③GR表達(dá)下調(diào)可引起其功能受抑制,特別是抗炎作用減弱,從而導(dǎo)致全身炎癥反應(yīng)綜合癥,可能是多器官功能衰竭的重要原因。
[Abstract]:Objective: to investigate the levels of serum cortisol cortisone, adrenocorticotropic hormone (ACTH), malondialdehyde (MDA) and superoxide dismutase (SOD) in rabbits after hepatic firearm injury at high altitude. The dynamic changes of glutathione glutathione (GSH) and glucocorticoid receptor (GRG) in lung tissue were studied in order to improve the comprehensive treatment level of liver trauma at high altitude and reduce the mortality of liver injury. It also provides theoretical basis for prevention and treatment of multiple organ dysfunction syndrome after firearm injury of liver at high altitude. Methods: 144 adult healthy New Zealand rabbits were randomly divided into two groups: group A with hepatic firearm injury in plain area, group B with firearm injury group with liver firearm injury at high altitude, group C with firearm injury group adapted to high altitude. Each group was subdivided into two groups: normal control group and normal control group, and 6 rats in each group for 72 h. The model of hepatic firearm injury was successfully established at different altitude. Blood samples and left lower lung tissues were collected at each time point, and serum Cor and ACTH were measured by solid phase sandwich enzyme-linked immunosorbent assay (Elisa). The content of serum MDA was determined by thiobarbituric acid method. The activity of serum SOD was determined by xanthine oxidase method. The content of GSH in whole blood of each group was determined by colorimetric quantitative method. The expression of gr was detected by immunohistochemical image analysis. Results compared with the normal group, the serum level of Cora ACTH in the three groups was still at a higher level at 6 h after firearm injury than that in the normal group at 12 h, and then decreased gradually. The content of MDA increased continuously at 12h, reached the peak value (P0.01A), then decreased gradually. The content of SOD increased slightly at the early stage of injury, and then decreased gradually. After reaching the lowest value, it gradually recovered; After injury, the expression of gr began to decrease, the lowest point appeared at 6 hours, and the slow recovery after 72 hours did not return to the normal level of 2.2.Compared with the firearm injury group in plain, the Cor in the normal group first entered the plateau. The content of ACTH was higher than that of the plain normal group (P 0.01), and the range of increase and the highest level after injury were higher than those in the normal group. MDA reached the peak level (P0.01) and SOD activity increased at the early stage and then reached the lowest level. The decrease and the lowest level of gr expression after injury were also more significant than those of plain firearm injury group, and the increase of Corn ACTH content and reaching the highest level in all groups at high altitude were better than those in plain firearm injury group. The increase of MDA and the decrease of GSH content in SOD activity were P0.05A; The decrease of gr expression and the lowest level of gr expression were more significant, but the significance of gr expression was lower than that of the new plateau group. Conclusion under the influence of trauma and high altitude hypoxic double stress, there was obvious stress response after firearm injury at high altitude, which was stronger than that in plain. (2) firearm injury broke the balance between oxidation and antioxidation in vivo. At the same time, hypoxia at high altitude could make the body produce excessive free radicals and decrease the ability of scavenging free radicals. Oxygen free radicals may be involved in the pathophysiological process of the body after firearm injury. The down-regulation of 3gr expression may result in the inhibition of its function, especially the weakening of its anti-inflammatory effect, which may lead to systemic inflammatory response syndrome, which may be an important cause of multiple organ failure.
【學(xué)位授予單位】：石河子大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類(lèi)號(hào)】：R826.5

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