CT引導(dǎo)下鹽酸溶液化學(xué)消融實(shí)驗(yàn)研究
發(fā)布時(shí)間：2019-05-23 10:00
【摘要】：目的 1、研究6mol/l鹽酸溶液局部注射對新西蘭大白兔機(jī)體的影響，探討6mol/l鹽酸溶液作為化學(xué)消融劑使用的可行性。 2、研究不同劑量鹽酸溶液作用于新西蘭大白兔肝臟、背部肌肉消融范圍的大小，觀察消融后不同階段組織壞死情況及周圍炎性反應(yīng)，明確消融區(qū)域組織學(xué)變化，總結(jié)機(jī)體對鹽酸消融區(qū)域修復(fù)規(guī)律。 材料和方法 1、在CT引導(dǎo)下將6mol/l鹽酸溶液用微量泵緩慢注入4只新西蘭大白兔前腿肌肉內(nèi)，每只2ml，速度為4ml/h，并在注射前后10min、注射后一周內(nèi)每天取兔耳中動(dòng)脈血化驗(yàn)，監(jiān)測肌酐(CR)、肌酸激酶(CK)、天冬氨酸氨基轉(zhuǎn)移酶(AST)、丙氨酸氨基轉(zhuǎn)移酶(ALT)等項(xiàng)目。觀察實(shí)驗(yàn)動(dòng)物基本生命體征，對實(shí)驗(yàn)室檢查結(jié)果進(jìn)行分析。 2、新西蘭大白兔24只，隨機(jī)分為8組，按照計(jì)劃分別將0.5ml、1.0ml、1.5ml、2.0ml鹽酸溶液分步驟以4ml/h的速度滴注入兔背部特定區(qū)域肌肉內(nèi)，前4組觀察術(shù)后12h內(nèi)消融區(qū)域的情況；后4組觀察術(shù)后4周內(nèi)鹽酸溶液消融區(qū)域的情況。實(shí)驗(yàn)結(jié)束時(shí)處死動(dòng)物，取材做病理切片，觀察消融范圍及其周圍炎性反應(yīng)和纖維化程度。 2、新西蘭大白兔8只，隨機(jī)分為2組并組內(nèi)編號，第一組肝內(nèi)注射0.5ml鹽酸溶液，第二組肝內(nèi)注射1.0ml鹽酸溶液，分別在注射后1天、1月處死實(shí)驗(yàn)動(dòng)物，取材做病理切片，觀察消融范圍及其周圍炎性反應(yīng)和纖維化程度。 結(jié)果 1.6mol/l鹽酸溶液2ml滴注入兔肌肉組織后，兔外周血實(shí)驗(yàn)室檢查可見肌酐、肌酸激酶、天冬氨酸氨基轉(zhuǎn)移酶、丙氨酸氨基轉(zhuǎn)移酶等指標(biāo)在術(shù)后3天內(nèi)一過性升高，后逐漸恢復(fù)正常。動(dòng)物一般情況良好，，注射局部皮膚潰爛，呈淡黃色。 2.大體病理結(jié)果顯示，0.5ml、1.0ml、1.5ml、2.0ml鹽酸溶液作用于肌肉，消融最大范圍分別為2.77±0.29、3.08±0.75、3.22±0.98、3.46±0.20厘米，約在注射鹽酸溶液12h后出現(xiàn)；12h內(nèi)壞死范圍隨鹽酸劑量的遞增、時(shí)間延長而不斷增大，壞死區(qū)域周圍可見粒細(xì)胞、多核巨細(xì)胞聚集；隨時(shí)間推移，12h-4w消融范圍逐漸縮小，3周后壞死組織內(nèi)可見大量的纖維細(xì)胞。 2.8只新西蘭大白兔肝內(nèi)定量注射鹽酸溶液后，基本生命體征平穩(wěn)。定期處死實(shí)驗(yàn)動(dòng)物，取材行病理學(xué)分析。大體病理顯示切面灰白色，質(zhì)地均勻；鏡下可見鹽酸注射區(qū)域壞死明顯，周圍可見炎性反應(yīng)區(qū)域。0.5ml鹽酸溶液作用于肝臟組織，消融24h后，消融范圍約為2.05cm,1月后約為1.72cm；1.0ml鹽酸溶液作用于肝臟組織，消融1月后，消融范圍約為2.75cm,1月后約為2.17cm。 結(jié)論 1、4只新西蘭大白兔經(jīng)6mol/l鹽酸溶液局部注射后，消融效果良好，肌酐、肌酸激酶、天冬氨酸氨基轉(zhuǎn)移酶、丙氨酸氨基轉(zhuǎn)移酶一過性升高，3天后機(jī)體經(jīng)肝臟、腎臟代謝可自行將外周血監(jiān)測指標(biāo)調(diào)整至正常范圍，實(shí)驗(yàn)兔基本生命體征平穩(wěn)。6mol/l鹽酸溶液在CT引導(dǎo)下作為消融劑使用安全可靠。 2、24只新西蘭大白兔肌肉滴注鹽酸溶液后，消融范圍內(nèi)細(xì)胞壞死徹底，消融范圍在12h內(nèi)隨時(shí)間延長而逐漸增大，消融后12小時(shí)至4周，消融范圍隨時(shí)間延長而縮小。范圍與劑量成正比，消融區(qū)域范圍可控，壞死徹底。 3、8只新西蘭大白兔肝臟滴注6mol/l鹽酸溶液后，未見明顯全身反應(yīng)，消融局部區(qū)域細(xì)胞核溶解，消融范圍外1cm區(qū)域內(nèi)匯管區(qū)可見壞死，消融范圍可控、細(xì)胞壞死徹底。
[Abstract]:Purpose 1. The effect of local injection of 6 mol/ l hydrochloric acid solution on the body of New Zealand white rabbits was studied. Sex.2. To study the size of different dose of hydrochloric acid solution on the liver and back muscle of New Zealand white rabbit, to observe the tissue necrosis and the surrounding inflammatory reaction at different stages of the ablation, to define the histological change of the ablation area, and to summarize the body's repair of the ablation area of hydrochloric acid. The law of complex. Materials and Methods 1.6 mol/ l hydrochloric acid solution was slowly injected into the front leg muscles of four New Zealand white rabbits under the guidance of CT, with a speed of 4 ml/ h and 10 min before and after the injection, and the arterial blood test and the monitor in the ear of rabbits were taken every day after the injection. myostatin (CR), creatine kinase (CK), aspartate aminotransferase (AST), alanine aminotransferase ( The items such as ALT were observed. The basic vital signs of the experimental animals were observed, and the laboratory test was performed. The results were analyzed. Twenty-four New Zealand white rabbits were randomly divided into 8 groups, 0.5 ml, 1.0 ml, 1.5 ml, and 2.0 ml of hydrochloric acid solution were injected into the specific area of the back of the rabbit at a rate of 4 ml/ h, respectively. The condition of the ablation area within h;4 weeks after the last 4 weeks, the hydrochloric acid was observed in 4 weeks after the operation The area of the solution was ablated. The animals were sacrificed at the end of the experiment, the materials were obtained for pathological sections, and the ablation range and the surrounding inflammation were observed. Response and fibrosis degree.8 New Zealand white rabbits were randomly divided into 2 groups and group numbers. The first group was injected with 0.5 ml of hydrochloric acid solution, and the second group was injected with 1.0 ml of hydrochloric acid solution respectively. To test the animal, make a pathological section for the materials, and observe the ablation scope and the week pericarditis Results: After 2 ml of 1.6 mol/ l hydrochloric acid solution was injected into the muscle tissue of the rabbit, the blood pressure of the rabbit's peripheral blood was examined and the indexes of creatine kinase, creatine kinase, aspartate aminotransferase and alanine aminotransferase were detected at 3. A transient increase in the day and a gradual return to normal. The animals generally have a good condition. The results showed that 0.5 ml, 1.0 ml, 1.5 ml, and 2.0 ml of hydrochloric acid were applied to the muscles. The maximum range of ablation was 2.77, 0.29, 3.08, 0.75, 3.22, 0.98, 3.46 and 0.20 cm, respectively, and then appeared after the injection of the hydrochloric acid solution for 12 h, and the area of necrosis over 12 h the dose of the hydrochloric acid is increased, the time is prolonged, and the peripheral visible granulocytes and the polynuclear giant cells of the necrotic area are gathered; over time, the ablation range of the 12h-4w is gradually reduced, A large number of fibroblasts were visible in the necrotic tissue after 3 weeks. 2.8 New Zealand white rabbits' liver After the internal quantitative injection of the hydrochloric acid solution, the basic vital signs were flat. Stable. The experimental animals were sacrificed on a regular basis, and the pathological analysis was performed on the materials. The gross pathology showed that the section was off-white and the texture was uniform; the area of the injection of the hydrochloric acid was visible under the microscope, and the surrounding area of the inflammatory reaction was observed. The 0.5 ml of the hydrochloric acid solution was applied to the liver tissue. After the ablation for 24 h, the ablation range was about 2.05 cm, and about 1.72 after 1 month. cm; 1.0 ml of hydrochloric acid solution was applied to the liver tissue, after 1 month of ablation, the ablation range was approximately 2 Conclusion 1. After local injection of 6 mol/ l hydrochloric acid solution,4 New Zealand white rabbits have good ablation effect, muscle strength, creatine kinase, and aspartate aminotransferase. Alanine aminotransferase increased transiently. After 3 days, the body had liver and kidney metabolism to adjust the peripheral blood monitoring index to the normal range, and the basic vital signs of the experimental rabbits were stable. / l Hydrochloric acid solution was safe and reliable as an ablation agent under the guidance of CT. Large,12-to-4-week post-ablation, and the ablation range extended over time The scope was in direct proportion to the dose, the area of the ablation was controlled and the necrosis was complete.3,8 New Zealand white rabbits were instilled with 6 mol/ l of hydrochloric acid in the liver, no obvious systemic reaction was observed, the nuclei of the local area of the ablation were dissolved, and the ablation scope was out of scope.
【學(xué)位授予單位】：中國人民解放軍醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R814.42
本文編號：2483807
[Abstract]:Purpose 1. The effect of local injection of 6 mol/ l hydrochloric acid solution on the body of New Zealand white rabbits was studied. Sex.2. To study the size of different dose of hydrochloric acid solution on the liver and back muscle of New Zealand white rabbit, to observe the tissue necrosis and the surrounding inflammatory reaction at different stages of the ablation, to define the histological change of the ablation area, and to summarize the body's repair of the ablation area of hydrochloric acid. The law of complex. Materials and Methods 1.6 mol/ l hydrochloric acid solution was slowly injected into the front leg muscles of four New Zealand white rabbits under the guidance of CT, with a speed of 4 ml/ h and 10 min before and after the injection, and the arterial blood test and the monitor in the ear of rabbits were taken every day after the injection. myostatin (CR), creatine kinase (CK), aspartate aminotransferase (AST), alanine aminotransferase ( The items such as ALT were observed. The basic vital signs of the experimental animals were observed, and the laboratory test was performed. The results were analyzed. Twenty-four New Zealand white rabbits were randomly divided into 8 groups, 0.5 ml, 1.0 ml, 1.5 ml, and 2.0 ml of hydrochloric acid solution were injected into the specific area of the back of the rabbit at a rate of 4 ml/ h, respectively. The condition of the ablation area within h;4 weeks after the last 4 weeks, the hydrochloric acid was observed in 4 weeks after the operation The area of the solution was ablated. The animals were sacrificed at the end of the experiment, the materials were obtained for pathological sections, and the ablation range and the surrounding inflammation were observed. Response and fibrosis degree.8 New Zealand white rabbits were randomly divided into 2 groups and group numbers. The first group was injected with 0.5 ml of hydrochloric acid solution, and the second group was injected with 1.0 ml of hydrochloric acid solution respectively. To test the animal, make a pathological section for the materials, and observe the ablation scope and the week pericarditis Results: After 2 ml of 1.6 mol/ l hydrochloric acid solution was injected into the muscle tissue of the rabbit, the blood pressure of the rabbit's peripheral blood was examined and the indexes of creatine kinase, creatine kinase, aspartate aminotransferase and alanine aminotransferase were detected at 3. A transient increase in the day and a gradual return to normal. The animals generally have a good condition. The results showed that 0.5 ml, 1.0 ml, 1.5 ml, and 2.0 ml of hydrochloric acid were applied to the muscles. The maximum range of ablation was 2.77, 0.29, 3.08, 0.75, 3.22, 0.98, 3.46 and 0.20 cm, respectively, and then appeared after the injection of the hydrochloric acid solution for 12 h, and the area of necrosis over 12 h the dose of the hydrochloric acid is increased, the time is prolonged, and the peripheral visible granulocytes and the polynuclear giant cells of the necrotic area are gathered; over time, the ablation range of the 12h-4w is gradually reduced, A large number of fibroblasts were visible in the necrotic tissue after 3 weeks. 2.8 New Zealand white rabbits' liver After the internal quantitative injection of the hydrochloric acid solution, the basic vital signs were flat. Stable. The experimental animals were sacrificed on a regular basis, and the pathological analysis was performed on the materials. The gross pathology showed that the section was off-white and the texture was uniform; the area of the injection of the hydrochloric acid was visible under the microscope, and the surrounding area of the inflammatory reaction was observed. The 0.5 ml of the hydrochloric acid solution was applied to the liver tissue. After the ablation for 24 h, the ablation range was about 2.05 cm, and about 1.72 after 1 month. cm; 1.0 ml of hydrochloric acid solution was applied to the liver tissue, after 1 month of ablation, the ablation range was approximately 2 Conclusion 1. After local injection of 6 mol/ l hydrochloric acid solution,4 New Zealand white rabbits have good ablation effect, muscle strength, creatine kinase, and aspartate aminotransferase. Alanine aminotransferase increased transiently. After 3 days, the body had liver and kidney metabolism to adjust the peripheral blood monitoring index to the normal range, and the basic vital signs of the experimental rabbits were stable. / l Hydrochloric acid solution was safe and reliable as an ablation agent under the guidance of CT. Large,12-to-4-week post-ablation, and the ablation range extended over time The scope was in direct proportion to the dose, the area of the ablation was controlled and the necrosis was complete.3,8 New Zealand white rabbits were instilled with 6 mol/ l of hydrochloric acid in the liver, no obvious systemic reaction was observed, the nuclei of the local area of the ablation were dissolved, and the ablation scope was out of scope.
【學(xué)位授予單位】：中國人民解放軍醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R814.42
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