【摘要】：目的:以神經(jīng)元和神經(jīng)膠質(zhì)細胞相關(guān)神經(jīng)生物學變化為切入點,探討有氧運動對慢性應激大鼠腦海馬區(qū)神經(jīng)可塑性的影響。方法:以SD大鼠為研究對象,按照體重均衡分為對照組(CON)、慢性應激模型組(MOD)、氟西汀組(FLU)、低強度運動組(LIR)和中等強度運動組(MIR)。給予大鼠4周慢性應激,并分別伴隨氟西汀給藥、低強度或者中等強度的運動干預。4周后,通過開場實驗、蔗糖飲水實驗、新奇抑制攝食實驗以及新物體識別實驗檢測有氧運動對慢性應激大鼠抑郁樣行為、焦慮樣行為以及認知障礙的影響;同時,利用酶聯(lián)免疫法和蛋白免疫印跡法,檢測有氧運動對慢性應激大鼠血清皮質(zhì)酮、海馬內(nèi)前炎因子IL1-β,以及蛋白GFAP、S100β、BDNF、p-ERK、p-CREB表達的影響,探討有氧運動抗抑郁的行為學效應和作用機制。結(jié)果:1)低強度和中等強度運動能夠顯著增加慢性應激大鼠的開場活動、縮短新奇抑制攝食潛伏期,同時提高慢性應激大鼠新物體的識別指數(shù);2)有氧運動有效逆轉(zhuǎn)了慢性應激大鼠血清皮質(zhì)酮的異常升高,同時降低海馬內(nèi)前炎性因子IL1-β的分泌;3)有氧運動可顯著提升膠質(zhì)細胞的標志性功能蛋白GFAP和S100β的表達水平,增強慢性應激大鼠的BDNF/ERK/CREB信號轉(zhuǎn)導通路中重要蛋白BDNF、p-ERK、pCREB的表達。結(jié)論:有氧運動可以通過提高慢性應激大鼠腦海馬區(qū)神經(jīng)可塑性來達到抗抑郁的目的�？赡芘c以下因素有關(guān):1)有氧運動調(diào)節(jié)血清皮質(zhì)酮的異常升高,降低海馬內(nèi)前炎性因子的釋放,同時,提高與神經(jīng)膠質(zhì)細胞相關(guān)蛋白GFAP和S100β活性,從而起到神經(jīng)元保護的作用;2)有氧運動可以通過激活CREB、BDNF以及ERK等重要信號蛋白的表達,提高神經(jīng)營養(yǎng)作用,促進神經(jīng)元的生長,增加神經(jīng)可塑性和神經(jīng)發(fā)生來發(fā)揮抗抑郁作用。
[Abstract]:Aim: to investigate the effects of aerobic exercise on neuroplasticity in the cerebral equine region of rats with chronic stress, based on the neurobiological changes associated with neurons and glial cells. Methods: SD rats were divided into two groups according to body weight balance: control group (CON), chronic stress model group (MOD), fluoxetine group (FLU), low intensity exercise group (LIR) and moderate intensity exercise group (MIR). Rats were given chronic stress for 4 weeks with fluoxetine administration, low or medium intensity exercise intervention. 4 weeks later, through the open field experiment, sucrose drinking test, the experimental results were as follows: (1) after 4 weeks, the rats were treated with fluoxetine for 4 weeks. The effects of aerobic exercise on depression-like behavior, anxiety-like behavior and cognitive impairment in chronic stress rats were detected by novel inhibition feeding test and new object recognition test. At the same time, the effects of aerobic exercise on the expression of serum corticosterone, IL1- 尾, GFAP,S100 尾 and BDNF,p-ERK,p-CREB in hippocampus of chronic stress rats were detected by enzyme linked immunosorbent assay (Elisa) and protein immunoblotting. To explore the behavioral effect and mechanism of aerobic exercise in anti-depression. Results: 1) low-intensity and moderate-intensity exercise could significantly increase the opening activity of chronic stress rats, shorten the novel inhibition of feeding latency, and increase the recognition index of new objects in chronic stress rats. 2) aerobic exercise can effectively reverse the abnormal increase of serum corticosterone and decrease the secretion of proinflammatory factor IL1- 尾 in the hippocampus of chronic stress rats. 3) aerobic exercise significantly increased the expression of GFAP and S100 尾 in glial cells, and increased the expression of BDNF,p-ERK,pCREB in the BDNF/ERK/CREB signal transduction pathway of chronic stress rats. Conclusion: aerobic exercise can improve neuroplasticity in cerebral equine region of chronic stress rats to achieve the purpose of anti-depression. It may be related to the following factors: 1) aerobic exercise regulates the abnormal elevation of serum corticosterone, reduces the release of proinflammatory factors in the hippocampus, and increases the activity of glial cell-associated proteins GFAP and S100 尾. Thus play the role of neuron protection; 2) aerobic exercise can play an antidepressant role by activating the expression of important signal proteins such as CREB,BDNF and ERK, enhancing neurotrophic effect, promoting the growth of neurons, increasing neuroplasticity and neurogenesis.
【作者單位】： 北京體育大學運動康復系;解放軍總醫(yī)院康復醫(yī)學中心;軍事醫(yī)學科學院;
【基金】：高等學校博士學科點專項科研基金(編號:20111112120002)
【分類號】：R87

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