【摘要】：目的:通過反復(fù)擊打兔股四頭肌的方法建立創(chuàng)傷性骨化性肌炎模型。方法:成年新西蘭大白兔9只,以自體左后肢為模型組,右后肢為對照組。用鋼球(質(zhì)量:0.25 kg)以自由落體(高度100 cm)方式擊打左后肢(模型組)股四頭肌中部,隨后每3天擊打1次,第4周后停止擊打,伸直位固定左膝關(guān)節(jié);右后肢(對照組)同樣方法固定。分別于第4、6、8周處死動物(均n=3),觀察腫脹度、局部體征、局部骨化情況。結(jié)果:模型組股四頭肌腫脹明顯,關(guān)節(jié)逐漸強直、僵硬,局部觸及硬結(jié);對照組關(guān)節(jié)僵直,局部未觸及硬結(jié)。影像學(xué)檢查示模型組第4、6、8周擊打處開始出現(xiàn)骨化影,對照組未出現(xiàn)。HE染色顯示8周時擊打處股四頭肌肌肉組織內(nèi)有明顯類軟骨或骨組織形成。結(jié)論:反復(fù)擊打股四頭肌的方法能成功建立單純肌肉損傷導(dǎo)致的創(chuàng)傷性骨化性肌炎動物模型。
[Abstract]:Objective: to establish traumatic myositis ossificans model by beating quadriceps femoris muscle repeatedly. Methods: nine adult New Zealand rabbits were treated with autologous left hindlimb as model group and right hind limb as control group. A steel ball (mass: 0. 25 kg) was used to strike the middle of the quadriceps femoris of the left hind limb (model group) with a free fall (100 cm), then every 3 days. After 4 weeks, the hit stopped and the left knee was straightened. The right hind limb (control group) was fixed with the same method. The animals were killed at the 6th week of the 4th week (all nun3) to observe the degree of swelling, local signs and local ossification. Results: in the model group, the quadriceps femoris were swollen, the joints became stiff and stiff, and the joints in the control group were stiff, but not in the control group. Imaging examination showed that in the model group, the ossification began to appear at the striking place at the 4th week of the 6th week, but not in the control group. HE staining showed that there was obvious cartilage or bone formation in the muscle tissue of the quadriceps femoris muscle at the 8th week. Conclusion: the method of beating quadriceps femoris repeatedly can successfully establish the animal model of traumatic ossification myositis caused by simple muscle injury.
【作者單位】： 云南省第一人民醫(yī)院(昆明理工大學(xué)附屬醫(yī)院);云南省普洱市中醫(yī)醫(yī)院骨傷科;云南省體育運動創(chuàng)傷�？漆t(yī)院運動醫(yī)學(xué)科;昆明理工大學(xué)醫(yī)學(xué)院;
【基金】：昆明理工大學(xué)引進人才基金項目(KKZ3201460023) 2013年云南省應(yīng)用基礎(chǔ)研究計劃項目(2013FZ182)資助
【分類號】：R-332;R873
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本文編號：2398329