【摘要】：目的:探討一次性向心運(yùn)動對白介素6(IL-6)抗炎效應(yīng)的影響,及PGC-1α在其中的角色。方法:2月齡雄性C57BL/6小鼠70只,建立一次性上坡跑運(yùn)動模型(速度10 m/min,坡度5°),分別選取運(yùn)動前(Control),運(yùn)動30 min和60 min(E30,E60),運(yùn)動恢復(fù)期30 min、60 min、3 h和6 h(PE30、PE60、PE3h、PE6h)為實(shí)驗(yàn)觀測點(diǎn),每組10只動物。二氯熒光素法檢測骨骼肌線粒體活性氧(ROS)生成速率,硫代巴比妥酸法檢測骨骼肌MDA含量,熒光定量PCR法檢測骨骼肌白細(xì)胞介素6(IL-6)、腫瘤壞死因子-α(TNF-α)、過氧化物酶體增殖活化受體γ共激活因子-1α(PGC-1α)和核因子-κB(NF-κB)p65 m RNA表達(dá)。結(jié)果:與Control組相較,ROS生成速率在E30、E60、PE30、PE60和PE3h組顯著升高(P0.01);MDA含量在各組均無顯著性變化(P0.05);IL-6 m RNA表達(dá)水平在E30、E60和PE30組顯著升高(P0.05);TNF-αm RNA表達(dá)水平在PE60和PE3h組顯著升高(P0.01);PGC-1αm RNA表達(dá)水平在E30、E60、PE30、PE60和PE3h組顯著升高(P0.01);NF-κB p65 m RNA表達(dá)水平在PE60和PE3h組顯著升高(P0.01)。結(jié)論:一次性向心運(yùn)動可誘導(dǎo)具有抗炎效應(yīng)的IL-6脈沖式表達(dá)升高。PGC-1α可能通過抑制ROS的氧化應(yīng)激損傷及NF-κB表達(dá)兩條途徑抑制炎癥,參與調(diào)節(jié)向心運(yùn)動中骨骼肌IL-6的抗炎效應(yīng)。
[Abstract]:Aim: to investigate the effect of one-time concentric exercise on the anti-inflammatory effect of interleukin 6 (IL-6) and the role of PGC-1 偽 in it. Methods: 70 male C57BL/6 mice aged 2 months were used to establish a one-off uphill running model (speed 10 m / min, slope 5 擄). (Control), was used for 30 min and 60 min (E30 E60) before exercise, respectively, and 30 min,60 min, during recovery period. The experimental sites were 3 h and 6 h (PE30,PE60,PE3h,PE6h) with 10 animals in each group. The production rate of reactive oxygen species (ROS) in mitochondria of skeletal muscle was detected by dichlorofluorescein method, the content of MDA in skeletal muscle by thiobarbituric acid method, and interleukin 6 (IL-6) in skeletal muscle by fluorescence quantitative PCR method. Tumor necrosis factor- 偽 (TNF- 偽), peroxisome proliferation-activated receptor 緯 -coactivator 1 偽 (PGC-1 偽) and nuclear factor- 魏 B (NF- 魏 B) p65 m RNA were detected. Results: compared with Control group, ROS production rate was significantly increased in E30 E60 PE30 PE60 group and PE3h group (P0.01); MDA content was not significantly changed in each group (P0.05); The expression level of IL-6 m RNA was significantly higher in E30 E60 and PE30 groups (P0.05), TNF- 偽 m RNA expression was significantly higher in PE60 and PE3h groups (P0.01). The expression of PGC-1 偽 m RNA was significantly increased in E30 E60 group and PE3h group (P0.01), and NF- 魏 B p65 m RNA level was significantly increased in PE60 and PE3h groups (P0.01). Conclusion: single concentric exercise can induce the increase of pulse expression of IL-6 with anti-inflammatory effect. PGC-1 偽 may inhibit inflammation by inhibiting oxidative stress injury of ROS and NF- 魏 B expression. Participate in regulating the anti-inflammatory effect of skeletal muscle IL-6 during concentric exercise.
【作者單位】： 北京體育大學(xué)研究生院;天津體育學(xué)院天津市運(yùn)動生理學(xué)與運(yùn)動醫(yī)學(xué)重點(diǎn)實(shí)驗(yàn)室;美國明尼蘇達(dá)大學(xué)運(yùn)動科學(xué)學(xué)院;
【基金】：國家自然科學(xué)基金重大國際合作項(xiàng)目(31110103919) 天津市應(yīng)用基礎(chǔ)與前沿技術(shù)研究計(jì)劃重點(diǎn)項(xiàng)目(14JCZDJC36600) 國家科技支撐計(jì)劃課題(2012BAD44B01-02) 天津市科技創(chuàng)新體系創(chuàng)新平臺計(jì)劃專項(xiàng)(10SYSYJC28400)
【分類號】：R87
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本文編號：2383093