發(fā)布時(shí)間：2018-11-09 21:05

【摘要】：核事故后，，對大量輻射暴露人群進(jìn)行迅速、準(zhǔn)確地傷情分類無論對于有限的醫(yī)療資源配置還是對大量傷員的分類、處理和早期治療都顯得至關(guān)重要。本文首先建立了高效液相色譜/電噴霧串聯(lián)質(zhì)譜用于大鼠體液中氨基酸分析的技術(shù)平臺(tái)，然后利用該技術(shù)平臺(tái)從劑量效應(yīng)和時(shí)間效應(yīng)兩方面，考察了急性電離輻射對大鼠體液中氨基酸代謝的影響，并結(jié)合非線性偏最小二乘法（PLS）進(jìn)行了傷情分類的研究。 1．建立了一個(gè)基于高效液相色譜/電噴霧串聯(lián)質(zhì)譜（HPLC-ESI-MS/MS）與固相萃取（SPE）相結(jié)合的用于分析大鼠體液中氨基酸的方法。以硅膠基質(zhì)的強(qiáng)陽離子交換固相萃取柱（SCX-SPE）提取體液中的氨基酸和降低基質(zhì)效應(yīng)，對SPE處理過程中的樣品pH值進(jìn)行了優(yōu)化。樣品pH值為2.8時(shí)，SCX-SPE提取氨基酸的回收率和重現(xiàn)性比較令人滿意。此外，對分析方法特性進(jìn)行了考察，包括精密度、準(zhǔn)確度及標(biāo)準(zhǔn)曲線等。 2.將建立的液質(zhì)聯(lián)用的大鼠血漿中氨基酸分析方法，從劑量效應(yīng)和時(shí)間效應(yīng)兩方面考察了電離輻射對大鼠血漿中氨基酸代謝的影響，結(jié)合非線性PLS回歸模型進(jìn)行了傷情分類研究。結(jié)果表明，輻射后5h血漿氨基酸對于無輻射對照組的分類正確率為92.3%；輻射后24h，不同程度的傷情分類正確率均在83%以上，其中中度、嚴(yán)重傷情的分類正確率分別達(dá)到了91.7%和92.3%；輻射后72h的傷情分類效果最佳，不同程度的傷情分類正確率均達(dá)到90%以上，其中中度和極重度的分類正確率達(dá)到100%。 3.將建立的液質(zhì)聯(lián)用的大鼠尿液中氨基酸分析方法，從劑量效應(yīng)和時(shí)間效應(yīng)兩方面考察了電離輻射對大鼠尿液中氨基酸代謝的影響，結(jié)合非線性PLS回歸模型進(jìn)行了傷情分類研究。結(jié)果表明，輻射后48h和72h的分類效果優(yōu)于前面時(shí)相點(diǎn)，不同程度輻射損傷的傷情分類正確率在70%以上，但整體分類效果不及血漿樣品。
[Abstract]:After the nuclear accident, it is very important to classify a large number of people exposed to radiation quickly and accurately, not only for the limited medical resources allocation, but also for the classification, treatment and early treatment of a large number of injured persons. In this paper, a high performance liquid chromatography (HPLC) / electrospray tandem mass spectrometry (HPLC-MS) platform was established for the analysis of amino acids in rat body fluid. The effects of acute ionizing radiation on amino acid metabolism in the body fluid of rats were investigated, and the classification of injury was studied with nonlinear partial least square method (PLS). 1. A method based on high performance liquid chromatography (HPLC) / electrospray tandem mass spectrometry (HPLC-ESI-MS/MS) combined with solid phase extraction (SPE) was developed for the analysis of amino acids in rat body fluid. The strong cation exchange solid phase extraction (SCX-SPE) column of silica gel matrix was used to extract amino acids from body fluid and to reduce the matrix effect. The pH value of the sample was optimized during SPE treatment. The recovery and reproducibility of amino acids extracted by SCX-SPE were satisfactory when the pH value of the sample was 2.8. In addition, the characteristics of the analytical method, including precision, accuracy and standard curve, are investigated. 2. The effects of ionizing radiation on the metabolism of amino acids in plasma of rats were investigated in terms of dose effect and time effect. The injury classification was studied with nonlinear PLS regression model. The results showed that the classification accuracy of plasma amino acids in the control group without radiation was 92.3% at 5 h after radiation. After 24 hours of radiation, the correct rate of classification of different degrees of injury was above 83%, among which, the correct rate of classification of severe injury was 91.7% and 92.3%, respectively. The classification effect of injury was the best at 72 hours after radiation. The correct rate of injury classification in different degrees was above 90%, and the classification accuracy of degree and severity reached 100%. 3. The effects of ionizing radiation on the metabolism of amino acids in urine of rats were investigated in terms of dose effect and time effect. The injuries were classified with nonlinear PLS regression model. The results showed that the classification effect of 48 h and 72 h after radiation was better than that of the former phase point. The classification accuracy of different degree of radiation injury was more than 70%, but the overall classification effect was not as good as plasma sample.
【學(xué)位授予單位】：蘇州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R818

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 鄭明辰;基于非線性偏最小二乘方法的急性電離輻射損傷早期傷情分類研究[D];蘇州大學(xué);2013年

本文編號(hào)：2321557