【摘要】：目的:觀察有氧運(yùn)動(dòng)對(duì)D-半乳糖(D-gal)誘導(dǎo)的腦衰老大鼠大腦前額葉突觸素(SYP)、突觸后致密區(qū)蛋白-95(PSD-95)表達(dá)的影響,探討有氧運(yùn)動(dòng)延緩腦衰老時(shí)學(xué)習(xí)記憶障礙的潛在機(jī)制。方法:將36只3月齡SD大鼠隨機(jī)分成生理鹽水對(duì)照組(C組)、D-gal組(D組)和D-gal加有氧運(yùn)動(dòng)組(DE組),每組12只。每日給予D、DE組大鼠1次腹腔注射D-gal(100 mg/kg/d),持續(xù)6周;DE組大鼠在D-gal注射期間進(jìn)行游泳訓(xùn)練(1 h/d,6 d/w)。然后利用Morris水迷宮檢測(cè)大鼠空間學(xué)習(xí)記憶能力,并采用免疫熒光、Western Blot和Real Time-PCR技術(shù)分別檢測(cè)大鼠大腦前額葉SYP、PSD-95蛋白分子及其m RNA表達(dá)水平。結(jié)果:(1)在Morris水迷宮實(shí)驗(yàn)定位航行訓(xùn)練過(guò)程中各大鼠的逃避潛伏期均逐漸下降,其中C和DE組訓(xùn)練第3天表現(xiàn)出最好成績(jī),其后無(wú)顯著性改變,D組逃避潛伏期則在第5天后降到最低,在第2、3、4天,D組平均逃避潛伏期明顯長(zhǎng)于C組和DE組(P0.05),而在第1、5、6天,各組大鼠間無(wú)顯著性差異(P0.05);在空間探索實(shí)驗(yàn)中,D組60 s內(nèi)穿越原平臺(tái)區(qū)域的次數(shù)明顯低于C和DE組(P0.05),而且后兩組大鼠有更長(zhǎng)時(shí)間停留在原平臺(tái)所在象限內(nèi)(P0.05)。(2)與C組相比,D組大鼠前額葉SYP蛋白分子及其SYP m RNA表達(dá)水平均顯著性降低(P0.01),DE組則顯著性高于D組(P0.05);D組大鼠前額葉PSD-95蛋白分子及其m RNA較C組顯著性下降(P0.01,P0.05);DE組PSD-95 m RNA與D組無(wú)顯著性差異,但其表達(dá)水平有所上升,并且DE組PSD-95蛋白分子顯著性高于D組(P0.05)。結(jié)論:有氧運(yùn)動(dòng)可在一定程度上抑制D-gal誘導(dǎo)的衰老過(guò)程中大鼠前額葉突觸蛋白SYP、PSD-95表達(dá)受損,減輕其下降程度,這可能是有氧運(yùn)動(dòng)延緩腦衰老學(xué)習(xí)記憶能力下降的分子機(jī)理之一。
[Abstract]:Aim: to observe the effect of aerobic exercise on the expression of presynaptic protein 95 (PSD-95) in prefrontal synaptophysin (SYP), in brain of aging rats induced by D-galactose (D-gal). To explore the potential mechanism of learning and memory impairment during aerobic exercise delaying brain aging. Methods: Thirty-six 3-month-old SD rats were randomly divided into normal saline control group (group C), D-gal group (group D) and D-gal plus aerobic exercise group (DE group) with 12 rats in each group. Rats in the DE group were treated with intraperitoneal injection of D-gal (100 mg/kg/d) daily for 6 weeks, while rats in the DE group underwent swimming training during D-gal injection (1 h / d / 6 d / w). Then the spatial learning and memory ability of rats was measured by Morris water maze, and the expression of SYP,PSD-95 protein and m RNA in the prefrontal lobe of rat brain were detected by immunofluorescence, Western Blot and Real Time-PCR techniques, respectively. Results: (1) the escape latency of the rats in the Morris water maze experiment decreased gradually during the navigation training. Group C and group DE showed the best results on the 3rd day of training, but there was no significant change after that. The average escape latency of group D was significantly longer than that of group C and DE (P0.05), but there was no significant difference between the two groups (P0.05). In the space exploration experiment, the times of crossing the original platform area in group D within 60 seconds were significantly lower than those in group C and DE (P0.05), and the latter two groups had a longer stay in the quadrant of the original platform (P0.05). (2) compared with group C. The expression of SYP protein and SYP m RNA in prefrontal lobe of D group was significantly lower than that in D group (P0.01), DE group was significantly higher than that in D group (P0.05). The expression of PSD-95 protein and its m RNA in prefrontal lobe of group D were significantly lower than those in group C (P0.01P 0.05). There was no significant difference in PSD-95 m RNA expression between DE group and D group, but the expression of PSD-95 protein in DE group was significantly higher than that in D group (P0.05). Conclusion: aerobic exercise can inhibit the expression of synaptophysin SYP,PSD-95 in the prefrontal lobe of rats during aging induced by D-gal to some extent, and reduce the degree of decrease in the expression of synaptophysin in the prefrontal lobe of rats. This may be one of the molecular mechanisms by which aerobic exercise delays the decline of brain aging learning and memory.
【作者單位】： 西南民族大學(xué)體育學(xué)院;成都體育學(xué)院運(yùn)動(dòng)醫(yī)學(xué)與健康學(xué)院;
【基金】：國(guó)家自然科學(xué)基金(31371202) 中央高�；究蒲袠I(yè)務(wù)費(fèi)專項(xiàng)資金(2017NZYQN22)
【分類號(hào)】：R87

【參考文獻(xiàn)】

相關(guān)期刊論文 前6條

1 付燕;王璐;李雪;楊澎湃;袁瓊嘉;;有氧運(yùn)動(dòng)對(duì)D-gal致腦衰老過(guò)程中大鼠學(xué)習(xí)記憶能力及PSD-95的影響[J];武漢體育學(xué)院學(xué)報(bào);2016年08期

2 付燕;謝攀;李雪;王璐;楊澎湃;袁瓊嘉;;長(zhǎng)期有氧運(yùn)動(dòng)對(duì)大鼠腦衰老過(guò)程中學(xué)習(xí)記憶與海馬BDNF表達(dá)的影響[J];中國(guó)運(yùn)動(dòng)醫(yī)學(xué)雜志;2015年08期

3 袁瓊嘉;張金梅;鄧文騫;王富鴻;李雪;王璐;;衰老過(guò)程中的運(yùn)動(dòng)干預(yù)對(duì)大鼠學(xué)習(xí)記憶能力及海馬神經(jīng)粘附分子表達(dá)的影響[J];體育科學(xué);2014年08期

4 Yang Liu;Zhanhua Liang;Jing Liu;Wei Zou;Xiaoyan Li;Yachen Wang;Lijia An;;Downregulation of caveolin-1 contributes to the synaptic plasticity deficit in the hippocampus of aged rats[J];Neural Regeneration Research;2013年29期

5 雷鳴;朱祖健;;D-半乳糖致衰老的研究進(jìn)展[J];解剖科學(xué)進(jìn)展;2011年01期

6 江樂(lè)盛;于建云;高林波;石泉;李俊祥;李娟娟;;多重腦震蕩對(duì)大鼠空間認(rèn)知行為的影響[J];中華神經(jīng)外科疾病研究雜志;2007年05期

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 付燕;張業(yè)廷;袁瓊嘉;羅笑;;有氧運(yùn)動(dòng)對(duì)D-半乳糖誘導(dǎo)的腦衰老大鼠前額葉SYP、PSD-95表達(dá)的影響[J];中國(guó)運(yùn)動(dòng)醫(yī)學(xué)雜志;2017年07期

2 張瑞萍;張波;錢帥偉;張安民;;運(yùn)動(dòng)干預(yù)改善腦學(xué)習(xí)記憶功能分子機(jī)制的研究進(jìn)展[J];中國(guó)體育科技;2017年02期

3 王穎;王欣卉;徐炳政;朱磊;佐兆杭;劉淑婷;宮雪;;蕓豆芽菜多酚對(duì)D-半乳糖致小鼠機(jī)體氧化及腎臟損傷的修復(fù)作用[J];食品科學(xué);2017年13期

4 桑菲;陳軍;;急性酒精中毒對(duì)記憶功能影響的功能性磁共振研究進(jìn)展[J];放射學(xué)實(shí)踐;2016年12期

5 付燕;王璐;李雪;楊澎湃;袁瓊嘉;;有氧運(yùn)動(dòng)對(duì)D-gal致腦衰老過(guò)程中大鼠學(xué)習(xí)記憶能力及PSD-95的影響[J];武漢體育學(xué)院學(xué)報(bào);2016年08期

6 蔡婧;袁瓊嘉;;60min/d游泳運(yùn)動(dòng)對(duì)大鼠腦衰老和海馬GAP-43表達(dá)的影響[J];體育世界(學(xué)術(shù)版);2016年08期

7 夏婕妤;樊艷玲;賈道勇;張夢(mèng)思;張巖巖;李靜;景鵬偉;王璐;王亞平;;當(dāng)歸多糖對(duì)D-半乳糖致衰老小鼠肝損傷的保護(hù)作用及其初步機(jī)制研究[J];中華肝臟病雜志;2016年03期

8 鄧秀婷;羅Y，

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