大鼠尺骨活體疲勞損傷后微損傷變化規(guī)律研究
發(fā)布時(shí)間：2018-10-13 13:36
【摘要】：目的：比較活體大鼠尺骨疲勞損傷實(shí)驗(yàn)后骨微破裂指標(biāo),確定建立活體大鼠皮質(zhì)骨微損傷模型參數(shù)指標(biāo)。 方法：根據(jù)四點(diǎn)彎曲原理,我們課題小組研制出與PLD-5010型疲勞損傷實(shí)驗(yàn)機(jī)相配套的平行四點(diǎn)彎曲夾具,應(yīng)用于疲勞損傷實(shí)驗(yàn)。選取40只7月齡雌性SD大鼠(由湘雅二醫(yī)院動(dòng)物實(shí)驗(yàn)室提供),體重260±15.6g,置于動(dòng)物實(shí)驗(yàn)室飼養(yǎng)1周后,隨機(jī)分為去卵巢組(OVX組)和假手術(shù)組(SHAM組),每組均20只。OVX組和SHAM組分別在手術(shù)后3周后,應(yīng)用PLD-5010型疲勞損傷實(shí)驗(yàn)機(jī)及四點(diǎn)彎曲夾具對(duì)大鼠右側(cè)尺骨中段行疲勞損傷試驗(yàn)。其中將OVX組、SHAM組根據(jù)疲勞時(shí)間長短分為OVX1W組,OVX2W組,SHAM1W組,SHAM2W組。最后將所有大鼠麻醉后放血處死,取右側(cè)尺骨行大塊組織堿性品紅染色、塑料包埋和磨片,測(cè)量骨微破裂各指標(biāo)和骨細(xì)胞密度等。 結(jié)果：各組微損傷形態(tài)比較：各組微破裂形態(tài)主要表現(xiàn)為微破裂。各組之間微破裂相關(guān)指標(biāo)比較：同一疲勞時(shí)間不同處理組,OVX組中微破裂長度、微破裂數(shù)量、微破裂密度、微破裂面密度相關(guān)指標(biāo)數(shù)值均高于SHAM組,有統(tǒng)計(jì)學(xué)差異(P0.05)；同一處理組不同疲勞時(shí)間,OVX2W組微破裂長度、微破裂面密度指標(biāo)數(shù)值均高于OVX1W組,有統(tǒng)計(jì)學(xué)差異(P0.05),微破裂數(shù)量及微破裂密度指標(biāo)數(shù)值無統(tǒng)計(jì)學(xué)差異(P0.05)；SHAM1W組與SHAM2W組之間微破裂相關(guān)指標(biāo)無統(tǒng)計(jì)學(xué)差異(P0.05)。各組骨細(xì)胞密度比較：OVX組均高于SHAM組,有統(tǒng)計(jì)學(xué)差異(P0.05)；OVX組之間有統(tǒng)計(jì)學(xué)差異(P0.05)；SHAM組之間無統(tǒng)計(jì)學(xué)差異(P0.05)。 結(jié)論：應(yīng)用PLD-5010型疲勞損傷實(shí)驗(yàn)機(jī)及平行四點(diǎn)彎曲夾具對(duì)大鼠尺骨行疲勞損傷實(shí)驗(yàn),能夠建立活體大鼠尺骨疲勞損傷模型；確定建立模型參數(shù)為每次最大載荷0.0555N/g體重,頻率4Hz,負(fù)載次數(shù)10000次,時(shí)間為2周,1周3次。 目的：建立大鼠皮質(zhì)骨微損傷模型后,觀測(cè)大鼠尺骨疲勞后不同時(shí)間骨微損傷變化,初步探討大鼠微損傷自身修復(fù)規(guī)律。 方法：根據(jù)第一部分實(shí)驗(yàn)結(jié)果建立骨質(zhì)疏松疲勞微損傷模型參數(shù),選取40只7月齡大鼠隨機(jī)分為4組,并將平行四點(diǎn)彎曲單夾具改良為雙夾具,予PLD-5010型疲勞損傷實(shí)驗(yàn)機(jī)及平行四點(diǎn)彎曲夾具對(duì)大鼠尺骨行疲勞損傷實(shí)驗(yàn),疲勞時(shí)間為2周。每組分別于疲勞損傷后1周、2周、3周以及4周處死,取大鼠右側(cè)尺骨,行大塊組織堿性品紅染色、塑料包埋和磨片,磨片測(cè)量骨微破裂指標(biāo)。 結(jié)果：各組之間微破裂相關(guān)指標(biāo)比較：各組微破裂形態(tài)主要表現(xiàn)為微破裂。OVX1W組與OVX2W組微破裂長度、微破裂數(shù)量、微破裂密度及微破裂面密度明顯高于OVX3W組及OVX4W組,具有統(tǒng)計(jì)學(xué)差異(P0.05)；OVX1W與OVX2W之間微破裂相關(guān)指標(biāo)數(shù)值無明顯差異性(P0.05),OVX3W及OVX4W微破裂相關(guān)指標(biāo)數(shù)值無明顯差異性(P0.05)。 結(jié)論：疲勞實(shí)驗(yàn)結(jié)束后,大鼠右側(cè)尺骨微破裂數(shù)量及長度隨時(shí)間變化明顯減少,2周到3周之間修復(fù)最為明顯,4周時(shí)骨微破裂逐漸修復(fù)。
[Abstract]:Aim: to compare the indexes of bone microrupture after ulnar fatigue injury in living rats and to determine the parameters of cortical microinjury model in vivo. Methods: according to the four-point bending principle, our team developed a parallel four-point bending fixture matched with PLD-5010 fatigue damage testing machine, which was applied to fatigue damage experiment. Forty 7-month-old female SD rats (provided by the animal laboratory of Xiangya No.2 Hospital), weighing 260 鹵15.6g, were placed in the animal laboratory for one week. Two groups were randomly divided into ovariectomized group (OVX group) and sham operation group (SHAM group) with 20 rats in each group. After 3 weeks of operation, the OVX group and SHAM group were subjected to the fatigue injury test of the middle segment of the right ulna using PLD-5010 type fatigue injury test machine and four-point bending clamp respectively. OVX group and SHAM group were divided into OVX1W group, OVX2W group, SHAM1W group and SHAM2W group according to fatigue time. At last, all the rats were bled out and killed after anesthesia. The right ulna was stained by alkaline fuchsin staining, plastic embedding and grinding, and the indexes of bone microrupture and the density of bone cells were measured. Results: the microfracturing morphology of each group was mainly microruptured. Comparison of microfracture correlation indexes among groups: the length, number, density and density of microrupture in OVX group were significantly higher than those in SHAM group with the same fatigue time (P0.05). Different fatigue time of the same treatment group, OVX2W group microfracture length, microfracture surface density index values were higher than OVX1W group, there was statistical difference (P0.05), the number of micro-rupture and micro-rupture density index values were not statistically different (P0.05); There was no statistical difference between SHAM1W group and SHAM2W group (P0.05). Comparison of bone cell density in each group: OVX group was higher than SHAM group, there was statistical difference (P0.05); OVX group there was statistical difference (P0.05); SHAM group there was no statistical difference (P0.05). Conclusion: the fatigue injury model of ulna of rats can be established by using PLD-5010 fatigue injury test machine and parallel four-point bending fixture, and the model parameters are determined to be maximum load 0.0555N/g weight per time. Frequency 4 Hz, load times 10000 times, time 2 weeks, 3 times a week. Aim: to observe the changes of bone microdamage after ulnar fatigue in rats and to explore the self-repair rule of microinjury in rats. Methods: according to the experimental results of the first part, the model parameters of osteoporosis fatigue microdamage were established. Forty 7-month-old rats were randomly divided into 4 groups, and the parallel four-point bending single clamp was modified into two clamps. The fatigue damage test of ulna of rats was carried out with PLD-5010 type fatigue damage test machine and parallel four point bending fixture. The fatigue time was 2 weeks. Each group was killed at 1 week, 2 weeks, 3 weeks and 4 weeks after fatigue injury. The right ulna of the rats were collected and stained with alkaline fuchsin. Results: the results showed that the microrupture morphology of each group was mainly microrupture, the length, number, density and surface density of microrupture in OVX1W group and OVX2W group were significantly higher than those in OVX3W group and OVX4W group. There was statistical difference (P0.05); there was no significant difference between OVX1W and OVX2W (P0.05); there was no significant difference between OVX3W and OVX4W (P0.05). Conclusion: after fatigue test, the number and length of right ulnar microrupture in rats decreased obviously with time, the repair was most obvious between 2 weeks and 3 weeks, and gradually repaired at 4 weeks.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R87
本文編號(hào)：2268836
[Abstract]:Aim: to compare the indexes of bone microrupture after ulnar fatigue injury in living rats and to determine the parameters of cortical microinjury model in vivo. Methods: according to the four-point bending principle, our team developed a parallel four-point bending fixture matched with PLD-5010 fatigue damage testing machine, which was applied to fatigue damage experiment. Forty 7-month-old female SD rats (provided by the animal laboratory of Xiangya No.2 Hospital), weighing 260 鹵15.6g, were placed in the animal laboratory for one week. Two groups were randomly divided into ovariectomized group (OVX group) and sham operation group (SHAM group) with 20 rats in each group. After 3 weeks of operation, the OVX group and SHAM group were subjected to the fatigue injury test of the middle segment of the right ulna using PLD-5010 type fatigue injury test machine and four-point bending clamp respectively. OVX group and SHAM group were divided into OVX1W group, OVX2W group, SHAM1W group and SHAM2W group according to fatigue time. At last, all the rats were bled out and killed after anesthesia. The right ulna was stained by alkaline fuchsin staining, plastic embedding and grinding, and the indexes of bone microrupture and the density of bone cells were measured. Results: the microfracturing morphology of each group was mainly microruptured. Comparison of microfracture correlation indexes among groups: the length, number, density and density of microrupture in OVX group were significantly higher than those in SHAM group with the same fatigue time (P0.05). Different fatigue time of the same treatment group, OVX2W group microfracture length, microfracture surface density index values were higher than OVX1W group, there was statistical difference (P0.05), the number of micro-rupture and micro-rupture density index values were not statistically different (P0.05); There was no statistical difference between SHAM1W group and SHAM2W group (P0.05). Comparison of bone cell density in each group: OVX group was higher than SHAM group, there was statistical difference (P0.05); OVX group there was statistical difference (P0.05); SHAM group there was no statistical difference (P0.05). Conclusion: the fatigue injury model of ulna of rats can be established by using PLD-5010 fatigue injury test machine and parallel four-point bending fixture, and the model parameters are determined to be maximum load 0.0555N/g weight per time. Frequency 4 Hz, load times 10000 times, time 2 weeks, 3 times a week. Aim: to observe the changes of bone microdamage after ulnar fatigue in rats and to explore the self-repair rule of microinjury in rats. Methods: according to the experimental results of the first part, the model parameters of osteoporosis fatigue microdamage were established. Forty 7-month-old rats were randomly divided into 4 groups, and the parallel four-point bending single clamp was modified into two clamps. The fatigue damage test of ulna of rats was carried out with PLD-5010 type fatigue damage test machine and parallel four point bending fixture. The fatigue time was 2 weeks. Each group was killed at 1 week, 2 weeks, 3 weeks and 4 weeks after fatigue injury. The right ulna of the rats were collected and stained with alkaline fuchsin. Results: the results showed that the microrupture morphology of each group was mainly microrupture, the length, number, density and surface density of microrupture in OVX1W group and OVX2W group were significantly higher than those in OVX3W group and OVX4W group. There was statistical difference (P0.05); there was no significant difference between OVX1W and OVX2W (P0.05); there was no significant difference between OVX3W and OVX4W (P0.05). Conclusion: after fatigue test, the number and length of right ulnar microrupture in rats decreased obviously with time, the repair was most obvious between 2 weeks and 3 weeks, and gradually repaired at 4 weeks.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R87
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相關(guān)期刊論文 前2條
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