【摘要】：目的探討應(yīng)用白介素-1受體拮抗蛋白(IL1RA)對(duì)早期創(chuàng)傷性關(guān)節(jié)炎軟骨細(xì)胞代謝的影響,主要觀察應(yīng)用后相關(guān)細(xì)胞炎性因子及蛋白酶基因的表達(dá)變化。方法 44只健康成年新西蘭大白兔,隨機(jī)分為對(duì)照(假手術(shù))組(CON組,n=4)、安慰劑組(PBS組,n=20)、IL1RA治療組(IL1RA組,n=20)。其中PBS組及IL1RA組均接受關(guān)節(jié)內(nèi)重物擊打,造成創(chuàng)傷性關(guān)節(jié)炎模型。造模后1 h,CON組不接受任何治療,PBS組及IL1RA組實(shí)驗(yàn)兔膝關(guān)節(jié)則分別給予0.5 m L無(wú)菌PBS和0.5 m L IL1RA-PBS關(guān)節(jié)腔注射。術(shù)后3 h及8 h采集關(guān)節(jié)液,檢測(cè)IL-1β含量。術(shù)后8 h處死動(dòng)物取軟骨組織進(jìn)行軟骨細(xì)胞培養(yǎng)后,測(cè)定細(xì)胞活力及凋亡率,RT-PCR檢測(cè)軟骨組織中ADAMTs-4、ADAMTs-5、MMP-3、IL-1β及TNF-α的m RNA表達(dá)。結(jié)果術(shù)后3 h及8 h,PBS組關(guān)節(jié)液中IL-1β的含量均明顯高于CON組(P0.01)。然而,經(jīng)IL1RA介入治療后,IL-1β在術(shù)后各測(cè)試點(diǎn)的含量均明顯低于PBS組(P0.05)。與CON組相比,術(shù)后8h培養(yǎng)的PBS組軟骨細(xì)胞活力明顯降低(P0.05),而IL1RA組軟骨細(xì)胞活力與CON組相比無(wú)顯著性差異,但明顯高于PBS組。另一方面,與CON組及IL1RA組相比,PBS組的細(xì)胞凋亡率明顯增高(P0.05)。而IL1RA組與CON組相比,細(xì)胞凋亡率無(wú)明顯差別(P0.05)。RT-RCR結(jié)果顯示,與CON組比較,PBS組所有目的基因m RNA的表達(dá)均明顯增加(P0.01)。然而,IL1RA組所有目的基因的表達(dá)與CON組相比無(wú)統(tǒng)計(jì)學(xué)差異,但明顯低于PBS組(P0.01)。結(jié)論實(shí)驗(yàn)結(jié)果表明創(chuàng)傷后關(guān)節(jié)炎早期應(yīng)用IL1RA介入治療可有效降低關(guān)節(jié)液細(xì)胞炎性因子的濃度,抑制細(xì)胞炎性因子對(duì)軟骨細(xì)胞生長(zhǎng)增殖的負(fù)性影響,降低細(xì)胞凋亡,且對(duì)于促進(jìn)軟骨細(xì)胞基質(zhì)降解的細(xì)胞炎性因子及蛋白酶的表達(dá)顯著下調(diào),對(duì)PTOA病程發(fā)展具有抑制作用。
[Abstract]:Objective to investigate the effect of interleukin-1 receptor antagonist protein (IL1RA) on the metabolism of chondrocytes in early traumatic arthritis. Methods 44 healthy adult New Zealand white rabbits were randomly divided into control (sham operation) group (CON group), placebo group (PBS group, n = 20) and IL1RA treatment group (IL1RA group, n = 20). PBS group and IL1RA group were all hit by intra-articular heavy objects to make traumatic arthritis model. One hour after modeling, the rabbits in the Con group were injected with 0.5 mL aseptic PBS and 0.5 mL IL1RA-PBS respectively in the knee joint without any treatment. Articular fluid was collected at 3 h and 8 h after operation to detect the content of IL-1 尾. Chondrocytes were harvested from chondrocytes 8 hours after operation, and the cell viability and apoptosis rate were measured. The m RNA expression of ADAMTs-4,ADAMTs-5,MMP-3,IL-1 尾 and TNF- 偽 in chondrocytes was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results the content of IL-1 尾 in articular fluid of PBS group was significantly higher than that of CON group at 3 h and 8 h postoperatively (P0.01). However, the level of IL-1 尾 in all test points after IL1RA intervention was significantly lower than that in PBS group (P0.05). Compared with CON group, the chondrocyte activity of PBS group was significantly lower than that of CON group (P0.05), but the chondrocyte activity of IL1RA group was not significantly different from that of CON group, but significantly higher than that of PBS group. On the other hand, compared with CON group and IL1RA group, the apoptosis rate of PBS group was significantly higher (P0.05). There was no significant difference in apoptosis rate between IL1RA group and CON group (P0.05). RT-RCR results showed that the expression of m RNA in CON group was significantly higher than that in CON group (P0.01). However, the expression of all target genes in the IL1RA group was not significantly different from that in the CON group, but was significantly lower than that in the PBS group (P0.01). Conclusion the experimental results show that early IL1RA intervention therapy can effectively reduce the concentration of inflammatory cytokines in articular fluid, inhibit the negative effects of cytokines on the growth and proliferation of chondrocytes, and reduce apoptosis. The expression of inflammatory cytokines and protease, which can promote the degradation of chondrocyte matrix, was significantly down-regulated and inhibited the progression of PTOA.
【作者單位】： 西安工業(yè)大學(xué)高水平運(yùn)動(dòng)管理中心;昆明學(xué)院體育學(xué)院;渭南市第一醫(yī)院重癥醫(yī)學(xué)科;
【分類號(hào)】：R873

【參考文獻(xiàn)】

相關(guān)期刊論文 前4條

1 羅韜;劉煬;范偉杰;;關(guān)節(jié)軟骨損傷基因治療研究進(jìn)展[J];中國(guó)矯形外科雜志;2016年08期

2 邵俊杰;張先龍;蔣W，

本文編號(hào)：2238860