【摘要】：目的：探討大鼠肺組織受照射后,血管內(nèi)皮細(xì)胞損傷導(dǎo)致放射性肺損傷發(fā)生、發(fā)展的可能致病機(jī)理。 方法：本研究選用60Coγ射線治療機(jī)單次照射大鼠右肺,照射劑量分別為OGy、7.OGy、14.4Gy,建立大鼠放射性肺損傷模型,選擇照射后1天(以下以d表示)、7d、30d、90d和180d為觀察時(shí)間點(diǎn),取對(duì)照組及各照射組大鼠的肺組織進(jìn)行相關(guān)檢測(cè),包括：①大鼠肺放射性損傷的病理學(xué)觀察,包括肉眼觀察、H-E染色及masson染色,光學(xué)顯微鏡觀察；②免疫組織化學(xué)方法檢測(cè)肺組織CD34、CD105、VEGF和FⅧ:RAg的表達(dá)水平。 結(jié)果：(1)肉眼觀察：對(duì)照組大鼠肺組織淡紅色,表面光滑,彈性好,7.0Gy組未見明顯的出血、水腫及纖維化改變；14.4Gy組早期可見片狀出血、水腫和腫脹,照射后90d,肺組織暗紅色,肺組織表面有白色斑點(diǎn)或斑片狀,照射后180d,大部分肺組織表面呈灰白色,肺體積縮小,肺表面凹凸不平,彈性明顯降低、質(zhì)地較硬,部分肺葉塌陷。(2)光鏡下病理組織學(xué)所見：對(duì)照組肺組織結(jié)構(gòu)正常；7.0Gy組照射后早期(1d、7d),肺泡腔及間隔可略見輕微滲出、充血和水腫；14.4Gy組肺泡腔及間隔可見滲出、充血、水腫和炎性細(xì)胞浸潤(rùn),局部可見紅細(xì)胞滲出；照射后30d,7.0Gy組肺泡腔及間隔出現(xiàn)輕微水腫和炎性細(xì)胞浸潤(rùn)；14.4Gy組見肺泡間隔增寬、水腫、炎性細(xì)胞浸潤(rùn)和出血,以及肺泡間隔及部分肺泡腔內(nèi)纖維素樣的滲出物,并且可見膠原形成；照射后90d,14.4Gy組肺泡間隔進(jìn)一步增寬,局部仍見出血,部分肺泡腔塌陷、不張、肺大泡形成并且出現(xiàn)膠原形成；照射后180d,14.4Gy組出現(xiàn)明顯的膠原形成和纖維化改變。(3)免疫組織化學(xué)檢測(cè)：①CD34,與正常對(duì)照組比較,14.4Gy組除1d外,其余各觀察時(shí)點(diǎn)均有顯著差異(P0.05),而7.0Gy組各時(shí)間點(diǎn)均無(wú)顯著性差異(P0.05)；②CD105,與正常對(duì)照組比較,7.0Gy組各時(shí)點(diǎn)均無(wú)顯著差異(P0.05);14.4Gy組除1d,90d外,其余各觀察時(shí)點(diǎn)均有顯著差異(P0.05)；③VEGF,與正常對(duì)照組比較,7.0Gy組無(wú)顯著差異(P0.05);14.4Gy組,除90d、180d有顯著差異(P0.05)外,其余時(shí)點(diǎn)均無(wú)顯著差異(P0.05)；④FVⅢ:RAg,與正常對(duì)照組比較,14.4Gy組從1d開始,各時(shí)點(diǎn)均有顯著差異(P0.05),而7.0Gy組各時(shí)間點(diǎn)均無(wú)顯著性差異(P0.05)。 結(jié)論：肺組織放射損傷后,微循環(huán)中成熟內(nèi)皮細(xì)胞數(shù)量的進(jìn)行性減少,是新生毛細(xì)血管生成障礙,纖維性修復(fù)慢性遷延的根本原因。
[Abstract]:Aim: to investigate the possible pathogenetic mechanism of radiation lung injury induced by vascular endothelial cell injury after irradiation in rat lung tissue. Methods: in this study, the right lung of rats was irradiated by 60Co 緯 -ray therapy machine for a single time. The radiation dose of OGy,7.OGy,14.4Gy, was used to establish the model of radiation-induced lung injury in rats. One day after irradiation, 30 days after irradiation, 90 days and 180 days after irradiation were selected as observation time points. The lung tissues of rats in the control group and each irradiation group were examined, including the pathological observation of lung radiation injury in 1 / 1 rat, including the observation of H-E staining and masson staining with naked eye, and the observation of optical microscope. 2 the expression of CD34,CD105,VEGF and F 鈪，

本文編號(hào)：2215820