【摘要】：目的通過(guò)建立大鼠正加速度(positive acceleration,+Gz)適應(yīng)性訓(xùn)練模型,觀察適應(yīng)性訓(xùn)練對(duì)各組大鼠接受正加速度處理后胃黏膜損傷程度影響,檢測(cè)大鼠胃黏膜內(nèi)前列環(huán)素(prostacyclin,PGI_2)、血栓素A2(thromboxane A2,TXA2)、TXA2/PGI_2比值、前列腺素E2(Prostaglandin E2,PGE_2)、環(huán)氧合酶-1 mRNA(COX-1 mRNA)和環(huán)氧合酶-2 mRNA(COX-2 mRNA)含量的變化,探討正加速度適應(yīng)性訓(xùn)練對(duì)大鼠胃黏膜保護(hù)作用的相關(guān)機(jī)制,從而為飛行員及航天員胃黏膜損傷相關(guān)疾病的防止提供理論依據(jù)。方法40只雄性SD大鼠隨機(jī)分為5組,每組8只,分別標(biāo)記為為A、B、C、D、E組。A組大鼠為空白對(duì)照,不做處理,B組大鼠+5Gz值暴露5min,每天一次,連續(xù)暴露5天,C組大鼠+10Gz值暴露5min,每天一次,連續(xù)暴露5天,D組大鼠適應(yīng)性訓(xùn)練(即+4Gz值暴露3min,每天1次,連續(xù)暴露5天)后+5Gz值暴露5min,每天1次,連續(xù)暴露5天,E組大鼠適應(yīng)性訓(xùn)練(即+4Gz值暴露3min,每天1次,連續(xù)暴露5天)后+10Gz值暴露5min,每天1次,連續(xù)暴露5天。試驗(yàn)結(jié)束后肉眼和光學(xué)顯微鏡下觀察胃黏膜損傷情況,計(jì)算損傷指數(shù),ELISA法檢測(cè)胃黏膜內(nèi)血栓素B_2(thromboxane B_2,TXB_2)、6-酮-前列腺素F1α(6-Keto-Prostaglandin F1α,6-Keto-PGF1α)、前列腺素E2(Prostaglandin E2,PGE_2)的含量,計(jì)算TXB_2/6-K-PGF1a的比值,RT-PCR法檢測(cè)胃黏膜內(nèi)COX-1 mRNA和COX-2 mRNA的相對(duì)表達(dá)量。結(jié)果(1)肉眼觀察,除A組外,其余各組胃黏膜均可見(jiàn)不同程度損傷。C組損傷指數(shù)高于B組(5.625±1.767 vs 1.750±0.707,P0.05)。適應(yīng)性訓(xùn)練后,D組損傷指數(shù)低于B組(0.875±0.641 vs 1.750±0.707,P0.05),E組損傷指數(shù)低于C組(2.250±1.035 vs 5.625±1.767,P0.05)。(2)B組TXB_2含量高于A組(251.018 pg/ml±50.845 pg/ml vs 121.400pg/ml±41.629 pg/ml,P0.05),C組TXB_2含量高于B組(331.538 pg/ml±79.102pg/ml vs 251.018 pg/ml±50.845 pg/ml,P0.05),適應(yīng)性訓(xùn)練后,D組TXB_2含量低于B組(159.588 pg/ml±36.216 pg/ml vs 251.018 pg/ml±50.845 pg/ml,P0.05),E組TXB_2含量低于C組(150.476 pg/ml±48.589 pg/ml vs 331.538 pg/ml±79.102pg/ml,P0.05)。B組6-Keto-PGF1a低于A組(52.015 pg/ml±11.827 pg/ml vs 106.322 pg/ml±16.909pg/ml,P0.05),C組6-Keto-PGF1a低于A組(44.726 pg/ml±18.867 pg/ml vs106.322 pg/ml±16.909 pg/ml,P0.05)。適應(yīng)性訓(xùn)練后,D組6-Keto-PGF1a高于B組(72.242 pg/ml±12.413 pg/ml vs 52.015 pg/ml±11.827 pg/ml,P0.05),E組6-Keto-PGF1a高于C組(87.426 pg/ml±15.833 pg/ml vs 44.726 pg/ml±18.867 pg/ml,P0.05)。B組TXB_2/6-K-PGF1a比值大于A組(5.128±1.788 vs 1.199±0.545,P0.05),C組TXB_2/6-K-PGF1a比值大于B組(8.599±4.157 vs 5.128±1.788,P0.05),適應(yīng)性訓(xùn)練后,D組TXB_2/6-K-PGF1a比值小于B組(2.283±0.705 vs 5.128±1.788,P0.05),E組TXB_2/6-K-PGF1a比值小于C組(2.250±1.035 vs 8.599±4.157,P0.05)。(3)B組PGE_2含量低于A組(60.468 pg/ml±9.697 pg/ml vs 81.462 pg/ml±20.340pg/ml,P0.05),C組PGE_2含量低于B組(24.598 pg/ml±6.017 pg/ml vs 60.468pg/ml±9.697 pg/ml,P0.05),適應(yīng)性訓(xùn)練后,E組PGE_2含量高于C組(46.165pg/ml±13.996 vs 24.598±6.017 pg/ml,P0.05)(4)COX-1 mRNA相對(duì)表達(dá)量各組間差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。B組COX-2mRNA表達(dá)高于A組(1.492±0.281 vs 0.978±0.109,P0.05),C組COX-2 mRNA表達(dá)高于A組和B組(2.642±0.320 vs 0.978±0.109,P0.05;2.642±0.320 vs1.492±0.281,P0.05)。適應(yīng)性訓(xùn)練后,D組COX-2 mRNA表達(dá)高于B組(1.782±0.099 vs 1.492±0.281,P0.05),E組COX-2 mRNA表達(dá)高于C組(3.268±0.083 vs 2.642±0.320,P0.05)。(5)各組6-Keto-PGF1α含量與COX-1 mRNA和COX-2 mRNA相對(duì)表達(dá)量間均無(wú)相關(guān)性,各組PGE_2含量與COX-1 mRNA和COX-2 mRNA相對(duì)表達(dá)量間均無(wú)相關(guān)性。結(jié)論正加速度適應(yīng)性訓(xùn)練可減輕高強(qiáng)度正加速度帶來(lái)的胃黏膜損傷,其機(jī)制與PGI_2、PGE_2含量升高、TXA2含量降低、TXA2/PGI_2比值降低及COX-2mRNA表達(dá)增加有關(guān)。
[Abstract]:Objective To observe the effects of positive acceleration (+Gz) on gastric mucosal injury after positive acceleration in rats, and to detect the prostacyclin (PGI_2), thromboxane A2 (TXA2), TXA2/PGI_2 ratio and prostaglandin E2 (P Rostaglandin E2, PGE_2, cyclooxygenase-1 mRNA (COX-1 mRNA) and cyclooxygenase-2 mRNA (COX-2 mRNA) levels were measured to investigate the protective effects of positive acceleration adaptive training on gastric mucosa in rats, so as to provide a theoretical basis for the prevention of gastric mucosal injury-related diseases in pilots and astronauts. Methods Forty male SD rats were randomly divided into two groups. Rats in group A were exposed to +5Gz for 5 minutes once a day for 5 days. Rats in group C were exposed to +10Gz for 5 minutes once a day for 5 consecutive days, once a day for 5 consecutive days. Rats in group D were exposed to +5Gz for 3 minutes, once a day for 5 consecutive days. Group E rats were exposed to + 10 Gz for 5 minutes, once a day for 5 days after adaptive training (i.e. +4 Gz exposure for 3 minutes, once a day for 5 consecutive days). The contents of E B_2, TXB_2, 6-keto-prostaglandin F1a (6-Keto-Prostaglandin F1a, 6-Keto-PGF_1a), prostaglandin E2 (PGE_2), the ratio of TXB_2/6-K-PGF_1a, and the relative expression of COX-1 mRNA and COX-2 mRNA in gastric mucosa were measured by RT-PCR. Results (1) The gastric mucosa of all groups except group A was observed with naked eyes. The injury index of group C was higher than that of group B (5.625 + 1.765 vs 1.767 vs 1.750 vs 1.750 vs 1.750 vs 0.707, P 0.05). After adaptive training, the injury index of group D was lower than that of group B (0.875 + 0.641 vs 1.750 vs 1.750 vs 1.700 vs 1.707, P 0.05) after adaptive training, the injury index of group E was lower than that of group C (2.250 + 1.250 + 1.035 vs 5.035.625 vs 5.625 5 5 5.625 + 1.767, P 0.05). (2) TXB_2 content in group B was higher than that in group A (251.018.018 pg/629 The TXB_2 content in group C was higher than that in group B (331.538 pg/ml (+79.102pg/ml (+79.102pg/ml (+ 79.102 pg/ml vs 251.018 pg/ml vs 251.018 pg/ml (+50.845 pg/ml, P 0.05)). After adaptive training, the TXB_2 content in group D was lower than that in group B (159.588 8 8 8 8 8 pg/ml (+36.216pg/ml (+36.216pg/ml +36.216pg/ml) vs 251.018 pg/ml (+50.845.845 pg/ml, P 0.05), TXB_2 content in group vs 331.538 pg/ml (+ 79.102 pg/ml), The 6-Keto-PGF1a in group B was lower than that in group A (52.015 pg/ml (11.827 pg / ml (16.909 pg / ml, P 0.05), 6-Keto-PGF1a in group C was lower than that in group A (44.726 pg / ml ml vs 52.015 pg/ml+11.827 pg/ml, P 0.05, 6-Keto-PGF1a in E group was higher than that in C group (87.426 pg/ml (+15.833 pg/ml (+15.833 pg/ml vs 44.726 pg/ml (+18.867 pg/ml (+18.867 pg/ml, P 0.05). TXB_2/6-K-PGF1a ratio in B group was higher than that in A group (5.128 (+1.128 [1.788 vs 1.788 vs 1.788 vs 1.199 [1.199 [0.545,P 0.05, P 0.05). TXB_2/6-K-PGF1a ratio in C group was higher than that in B group (8.599 [(8.599 The ratio of TXB_2/6-K-PGF1a in group D was lower than that in group B (2.283+0). The ratio of TXB_2/6-K-PGF1a in E group was lower than that in C group (2.250 (+ 1.035 vs 8.599 (+ 4.157, P 0.05). (3) The content of PGE_2 in B group was lower than that in A group (60.468 pg/ml (+ 9.697 PG vs vs 9.697 pg/ml (+ 9.697 pg/ml vs vs vs 81.462 pg/ml [81.462 pg/ml [20.340 pg/ml [20.340 pg/ml, P 0.05). (3) The content of PGE_2 in E_2 in E_2 was lower than that in C group (24.598 P8 pg/ml (+9.697 pg/ml, P 0.05). After adaptive training, E. The content of PGE_2 in group B was higher than that in group A (46.165 pg/ml (+13.996 vs 24.598 +6.017 pg/ml, P 0.05) (4) There was no significant difference in the relative expression of COX-1 mRNA between groups (P 0.05). The expression of COX-2 mRNA in group B was higher than that in group A (1.492 +0.281 vs 0.978 +0.109, P 0.05). After adaptive training, the expression of COX-2 mRNA in group D was higher than that in group B (1.782.099 vs 1.492.0.281, P 0.05). The expression of COX-2 mRNA in group E was higher than that in group C (3.268.083 vs 2.642.320, P 0.05). Conclusion Positive acceleration adaptive training can alleviate gastric mucosal injury induced by high intensity positive acceleration. The mechanism is related to the increase of PGI_2 and PGE_2 contents, the decrease of TXA2 content, the decrease of TXA2/PGI_2 ratio and the increase of COX-2 mRNA expression.
【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R856.5
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本文編號(hào)：2183480