【摘要】：目的:以SD大鼠作為實驗對象,研究運動和二惡英染毒對SD大鼠肝組織Nrf2及相關(guān)蛋白表達的影響。方法:將40只7周齡雄性大鼠適應(yīng)性喂養(yǎng)一周后隨機分為靜養(yǎng)對照組(NC),運動對照組(EC),染毒靜養(yǎng)組(NT),染毒運動組(ET)。NT,ET腹腔注射6.4μg/kg體重的TCDD;NC,EC大鼠注射等量的玉米油,每隔一周給予上述劑量的21%TCDD染毒維持劑量,持續(xù)染毒共8周。EC,ET進行8周的運動,運動方案為大鼠尾部負重5%體重,進行游泳運動,每次運動30分鐘,每周運動5天。實驗結(jié)束取材,檢測大鼠體重,肝臟重量,肝臟相對重量,檢測大鼠血清AST、ALT的活性,檢測Keap1、Nrf2蛋白含量,檢測Nrf2-ARE結(jié)合活性。結(jié)果:染毒對大鼠肝濕重的影響有主效應(yīng)(P=0.049);染毒對大鼠肝濕重/體重的影響有主效應(yīng)(P=0.049);運動對大鼠血清AST活性的影響有主效應(yīng)(P=0.020),染毒對大鼠血清AST活性的影響有主效應(yīng)(P=0.001);運動對血清ALT活性的影響有主效應(yīng)(P=0.001),染毒對血清ALT活性的影響有主效應(yīng)(P=0.049)。NC組和EC組大鼠肝細胞排列整齊,細胞結(jié)構(gòu)完整,NT組大鼠細胞排列紊亂,伴有炎癥細胞浸潤,NE組大鼠細胞排列稍顯紊亂,伴有炎癥細胞浸潤;運動對肝臟Nrf2總蛋白的表達有主效應(yīng)(P=0.004),染毒對肝臟Nrf2總蛋白的表達有主效應(yīng)(P=0.004);運動對肝臟Nrf2核蛋白的表達主效應(yīng)(P=0.000),染毒對肝臟Nrf2核蛋白的表達有可靠的主效應(yīng)(P=0.015),運動和染毒對肝臟Nrf2核蛋白的表達有交互效應(yīng)(P=0.024);運動對肝臟細胞核Nrf2-ARE結(jié)合活性有主效應(yīng)(P0.001);染毒對肝臟細胞核Nrf2-ARE結(jié)合活性有主效應(yīng)(P0.001);運動和染毒對肝臟細胞核Nrf2-ARE結(jié)合活性有可靠的交互效應(yīng)(P=0.043)結(jié)論:1.TCDD染毒使大鼠肝臟Nrf2核蛋白含量降低,抑制機體氧化應(yīng)激水平;2.運動可以緩解TCDD誘導(dǎo)的肝臟Nrf2核蛋白含量降低,增強機體氧化應(yīng)激能力;3.運動可以通過上調(diào)Nrf2及其相關(guān)蛋白的表達來有效降低TCDD導(dǎo)致的肝臟損傷。
[Abstract]:Aim: to study the effects of exercise and dioxin exposure on the expression of Nrf2 and related proteins in the liver of SD rats. Methods: forty 7-week-old male rats were randomly divided into control group (NC), exercise control group (EC), (NT), control group (ET). NTX et) after one week of adaptive feeding. The rats were injected with the same amount of corn oil by intraperitoneal injection of 6.4 渭 g/kg weight TCDDNCCEC rats. Every other week, the above dose of 21%TCDD was administered to maintain dose, and continuous exposure lasted for 8 weeks. The exercise program was 5% body weight bearing on the tail of the rats, swimming for 30 minutes each time, 5 days a week. At the end of the experiment, the body weight, liver weight and relative weight of liver were measured. The activity of alt, the protein content of Keap1Nrf2 and the binding activity of Nrf2-ARE were detected. Results: there were main effects on liver wet weight (P0.049), main effect on wet weight / body weight of rat liver (P0. 049), main effect on serum AST activity of rats (P0. 020) and effect on serum AST activity of rats after exposure to DW (P0. 020), P0. 049 (P0. 049), P0. 020 (P0. 020) and P0. 020 (P0. 020) respectively. The effect of exercise on serum ALT activity was significant (P0. 001), and the effect of exposure on serum ALT activity was significant (P0. 049). The hepatocytes of NC group and EC group were arranged neatly. In the NT group, the cell arrangement was disordered, and the inflammatory cell infiltration was found in the NE group. The cells in the NE group were slightly disordered, and the inflammatory cell infiltration was also found in the NE group. Exercise has a major effect on the expression of total Nrf2 protein in liver (P0. 004), a major effect on the expression of total protein of Nrf2 in liver (P0. 004), a major effect of exercise on the expression of Nrf2 nucleoprotein in liver (P0. 000), a reliable major effect on the expression of Nrf2 nuclear protein in liver (P0. 015). Exercise and exposure had an interactive effect on the expression of Nrf2 nucleoprotein in liver (P0. 024); exercise had a major effect on nuclear Nrf2-ARE binding activity of liver (P0. 001); exposure to exercise had a major effect on Nrf2-ARE binding activity in liver nucleus (P0. 001); exercise and exposure had a major effect on Nrf2-ARE binding activity in liver nucleus (P0. 001); exercise and exposure had a major effect on Nrf2-ARE binding activity in liver nucleus (P0. 001). Conclusion: 1. TCDD can reduce the content of Nrf2 nuclear protein in rat liver. Inhibition of oxidative stress level 2. Exercise can alleviate the decrease of Nrf2 nuclear protein content in liver induced by TCDD, and enhance the oxidative stress ability of the body. Exercise can effectively reduce liver injury induced by TCDD by up-regulating the expression of Nrf2 and its associated proteins.
【學(xué)位授予單位】：北京體育大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R87

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