本文選題：運動性疲勞 + 自由基��； 參考：《湖南師范大學》2016年碩士論文

【摘要】：1研究目的本實驗結合自由基疲勞理論和羰基毒化的研究成果,采用力竭疲勞模型,觀察4周中等強度運動、8周中等強度運動及各自結合灌胃人參皂甙Rg1,對力竭狀態(tài)大鼠骨骼肌和腦組織羰基化蛋白含量的影響。從去羰基應激的角度,初步探討人參皂Rg1抗運動性疲勞的作用機制。2研究方法實驗對象為清潔級SD大鼠40只,隨機分為4周安靜組(Q4)、4周運動組(E4)、4周安靜給藥組(QR4)、4周運動給藥組(ER4)、8周安靜組(Q8)、8周運動組(E8)、8周安靜給藥組(QR8)、8周運動給藥組(ER8),每組5只。運動給藥組和安靜給藥組大鼠按照30mg/kg體重劑量灌胃人參皂甙Rg1溶液,運動組和安靜組大鼠灌胃等容積生理鹽水。運動組與運動給藥組大鼠接受訓練周期分別為4周和8周,負荷為25m·min-1×30min的水平跑臺運動訓練。運動方案結束后,記錄力竭時間,并即刻取材。測定大鼠血清中血乳酸(LA)含量;測定大鼠骨骼肌和腦組織中超氧化物歧化酶(SOD)活性、過氧化氫酶(CAT)活性、谷胱甘肽過氧化物酶(GSH-Px)、丙二醛(MDA)含量以及羰基化蛋白的含量;3研究結果3.1體重測定結果實驗后,各組SD大鼠體重均顯著增加;E4組與ER4組均顯著低于QR4組(P0.05);E8組和ER8組顯著低于Q8組(P0.05)、QR8組(P0.01);其余均無顯著性差異。3.2力竭時間測定結果與Q4組比較,E4組和ER4組顯著延長(P0.01);與QR4組比較,ER4組顯著延長(P0.01);與E4組比較,ER4組力竭時間顯著延長(P0.01)。與Q8組比較,E8組和ER8組顯著延長(P0.01);與QR8組比較,ER8組顯著延長(P0.01)。與E8組比較,ER8組顯著延長(P0.01)。E8組力竭時間顯著長于E4組(P0.01),ER8組力竭時間顯著長于ER4組(P0.01)。其余均無顯著性差異。3.3血乳酸測定結果與Q4組比較,E4組和ER4組顯著降低(P0.01);與QR4組比較,ER4組顯著降低。與Q8組比較,E8組和ER8組顯著降低(P0.01);與QR8組比較,ER8組顯著降低(P0.01)。其余均無顯著性差異。3.4大鼠骨骼肌中抗氧化酶活性、MDA含量、羰基化蛋白含量與Q4比較,E4組CAT活性顯著升高(P0.05),MDA含量、羰基化蛋白含量顯著降低(P0.01);與QR4組比較,ER4組SOD活性、GSH-Px活性和CAT活性顯著升高(P0.05),MDA含量、羰基化蛋白含量顯著降低(P0.05);與E4組比較,ER4組SOD活性顯著升高(P0.05),MDA含量顯著降低(P0.05);與Q8組比較,E8組GSH-Px活性和CAT活性顯著升高(P0.01,P0.05),MDA含量、羰基化蛋白含量顯著降低(P0.01);與QR8組比較,ER8組SOD活性、GSH-Px活性和CAT活性顯著升高(P0.01),MDA含量、羰基化蛋白含量顯著降低(P0.01);與E8組比較,ER8組SOD活性(P0.01)、GSH-Px活性和CAT活性顯著升高(P0.05),MDA含量和羰基化蛋白含量顯著降低(P0.05)。ER8組SOD活性顯著高于ER4組(P0.05);在GSH-Px活性方面,E8組顯著高于E4組(P0.05),ER8組顯著高于ER4組(P0.05)。其余均無顯著性差異。3.5大鼠腦組織中抗氧化酶活性、MDA含量、羰基化蛋白含量與Q4組比較,E4組羰基化蛋白含量顯著降低(P0.01)。與QR4組比較,ER4組SOD活性和CAT活性顯著升高(P0.01,P0.05),MDA含量、羰基化蛋白含量顯著降低(P0.05,P0.01);與E4組比較,ER4組SOD活性顯著升高(P0.01)。與Q8組比較,E8組SOD活性(P0.01)、GSH-Px活性(P0.01)和CAT活性(P0.05)顯著升高,MDA含量、羰基化蛋白含量顯著降低(P0.01)。與QR8組比較,ER8組SOD活性、GSH-Px活性和CAT活性顯著升高(P0.01),MDA含量、羰基化蛋白含量顯著降低(P0.01)。與E8組比較,ER8組SOD活性、GSH-Px活性顯著升高(P0.01,P0.05),MDA含量和羰基化蛋白含量顯著降低(P0.05)。ER8組SOD活性、GSH-Px活性、CAT活性均顯著高于ER4組(P0.05);ER8組MDA含量顯著低于ER4組(P0.05)。其余均無顯著性差異。4結論4.1長期運動訓練可引起大鼠機體抗氧化酶活性適應性增強,提高機體對抗氧化應激和羰基應激能力,減少機體體內羰基化蛋白的生成。4.2人參皂甙Rg1可引起運動大鼠機體抗氧化酶活性適應性增強,提高機體對抗氧化應激和羰基應激能力,減少機體體內羰基化蛋白的生成。4.3運動訓練及結合人參皂甙Rg1均可提高大鼠運動能力,延長力竭運動時間。
[Abstract]:1 the purpose of this study was to combine the results of free radical fatigue theory and carbonyl toxicity, using exhaustive fatigue model, to observe the 4 week moderate intensity exercise, 8 weeks of moderate intensity exercise and the effect of each combination of gavage ginsenoside Rg1 on the carbonylation of egg white in the skeletal muscle and brain tissue of the exhausted rats. The effect mechanism of ginseng soap Rg1 on exercise fatigue resistance was studied by.2, which were divided into 40 clean grade SD rats, randomly divided into 4 weeks quiet group (Q4), 4 week exercise group (E4), 4 week quiet administration group (QR4), 4 week exercise group (ER4), 8 week quiet group (Q8), 8 week exercise group (E8), 8 weeks' quiet administration group (QR8), 8 week exercise group (ER8), 5 each 5 The rats in the exercise group and the quiet administration group were given the Rg1 solution of ginsenoside according to the 30mg/kg weight dose, the exercise group and the quiet group rats were filled with saline. The training period of the exercise group and the exercise group was 4 weeks and 8 weeks respectively, the load was 25m min-1 x 30min, and the exercise program was finished, after the exercise program was finished, The content of serum lactate (LA) in rat serum was measured and the content of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) content and carbonylation protein in the skeletal muscle and brain tissue of rats were measured, and the results of 3 study results of 3.1 body weight test were tested. The weight of SD rats in group E4 and ER4 group was significantly lower than that of group QR4 (P0.05), and E8 and ER8 groups were significantly lower than group Q8 (P0.05) and QR8 group (P0.01). The 4 groups were significantly prolonged (P0.01). Compared with the Q8 group, the E8 group and the ER8 group were significantly prolonged (P0.01). Compared with the QR8 group, the ER8 group was significantly longer (P0.01). The ER8 group was significantly longer than the E8 group (P0.01) and the duration of the exhaustion of the.E8 group was significantly longer than that of the group. Compared with group Q4, group E4 and group ER4 significantly decreased (P0.01). Compared with group QR4, ER4 group decreased significantly. Compared with group Q8, E8 group and ER8 group decreased significantly (P0.01). Compared with QR8 group, ER8 group significantly decreased. Group CAT activity increased significantly (P0.05), MDA content and carbonylation protein content decreased significantly (P0.01). Compared with QR4 group, SOD activity in group ER4, GSH-Px activity and CAT activity increased significantly (P0.05), MDA content, and carbonylation protein content significantly decreased (P0.05). Compared with group E8, the activity of GSH-Px and CAT activity increased significantly (P0.01, P0.05), MDA content, and carbonylation protein content significantly decreased (P0.01). Compared with QR8 group, SOD activity of ER8 group, GSH-Px activity and CAT activity increased significantly, and the content of carbonylation protein decreased significantly. The content of MDA and carbonylation protein decreased significantly (P0.05), and the activity of SOD in group.ER8 was significantly higher than that in group ER4 (P0.05). In GSH-Px activity, the E8 group was significantly higher than the E4 group (P0.05), and the ER8 group was significantly higher than that of the group (P0.05). Compared with group Q4, the content of carbonylation protein in group E4 decreased significantly (P0.01). Compared with group QR4, SOD activity and CAT activity in ER4 group increased significantly (P0.01, P0.05), MDA content, and carbonylation protein content significantly decreased (P0.05, P0.01). CAT activity (P0.05) increased significantly, MDA content and carbonylation protein content decreased significantly (P0.01). Compared with QR8 group, SOD activity in group ER8, GSH-Px activity and CAT activity increased significantly (P0.01), MDA content, and carbonylation protein content significantly decreased (P0.01). The protein content significantly decreased (P0.05).ER8 group SOD activity, GSH-Px activity, CAT activity was significantly higher than ER4 group (P0.05), ER8 group MDA content was significantly lower than ER4 group (P0.05). The rest no significant difference in.4 conclusion 4.1 long-term exercise training can cause the adaptation to antioxidant enzyme activity in rats, improve the body against oxidative stress and carbonyl stress energy. Force, reducing the formation of.4.2 ginsenoside Rg1 in the body of body carbonylation protein can cause the activity of anti oxidative stress and carbonyl stress in the body of the exercise rats, reduce the activity of carbonylation of protein in the body and reduce the.4.3 exercise training and the combination of human ginseng saponins Rg1, which can increase the exercise ability of rats and prolong the exercise ability of rats. Exhausting exercise time.
【學位授予單位】：湖南師范大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R87
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本文編號：2096013