本文選題：有氧運(yùn)動(dòng) + EGCG��； 參考：《北京體育大學(xué)》2012年碩士論文

【摘要】：研究目的：觀察運(yùn)動(dòng)以及運(yùn)動(dòng)聯(lián)合EGCG或(和)左旋肉堿的減肥效果,探索其對(duì)脂肪代謝和能量代謝的影響。 研究方法：以4周齡雄性SD大鼠為研究對(duì)象,高脂飼料誘導(dǎo)飲食型肥胖模型。然后進(jìn)行6周減肥干預(yù)實(shí)驗(yàn)：將肥胖大鼠隨機(jī)分為肥胖模型組(F,n=6)、單純運(yùn)動(dòng)組(S,n=6)、運(yùn)動(dòng)聯(lián)合EGCG組(E,n=6)、運(yùn)動(dòng)聯(lián)合LC組(L,n=6)、運(yùn)動(dòng)聯(lián)合EGCG和LC(EL，，n=6),仍然給予高脂飼料；運(yùn)動(dòng)采取中低強(qiáng)度跑臺(tái)運(yùn)動(dòng)方式,EGCG和LC按80mg/kg bw劑量灌胃。實(shí)驗(yàn)結(jié)束后測(cè)試各組血脂、肝臟脂酶活性以及附睪脂肪HSL和褐色脂肪UCPl蛋白表達(dá)水平。 研究結(jié)果：1)與F組相比,各干預(yù)組大鼠體重明顯降低(P0.05)：其中EL組體重較S組明顯下降(P0.05),其余各組之間沒有差異(P0.05)。2)各干預(yù)組血清TG顯著低于F組(P0.01),TC呈降低趨勢(shì)(P0.05), HDL-C為增加趨勢(shì)(P0.05)。與S組相比,E組、L組、EL組TG顯著降低(P0.01),而EL組TC和HDL-C明顯改善(分別為P0.05,P0.01),其余各組之間沒有明顯區(qū)別(P0.05)。3)與F組相比,E組、L組、EL組顯著提高了肝臟脂酶活性(P0.01,P0.05,P0.01)：其中E組、EL組的脂酶活性明顯高于S組(P0.05),同時(shí)與L組相比亦明顯增加(P0.05)。4)各干預(yù)組HSL蛋白水平顯著高于F組(P0.01)。E組HSL蛋白表達(dá)高于S組(P0.05),其他各組之間沒有明顯差異(P0.05)。5)與F組相比,S組和L組UCPl蛋白表達(dá)沒有明顯差異(P0.05),僅E組和EL組明顯提高了褐色脂肪UCP1蛋白表達(dá)(P0.05)。 研究結(jié)論：1)6周運(yùn)動(dòng)或運(yùn)動(dòng)聯(lián)合EGCG或(和)LC均可降低肥胖大鼠的體重,提高肝臟脂酶活性并改善血脂代謝,增強(qiáng)脂肪組織HSL蛋白表達(dá)促進(jìn)脂肪動(dòng)員和氧化利用。2) EGCG表現(xiàn)出提高褐色脂肪UCP1蛋白表達(dá)的能力,有氧運(yùn)動(dòng)或LC對(duì)BAT生熱作用沒有影響。3) EGCG在提高肝脂酶、改善血脂和增強(qiáng)HSL蛋白表達(dá)方面與運(yùn)動(dòng)有協(xié)同促進(jìn)作用,而LC僅協(xié)同促進(jìn)運(yùn)動(dòng)對(duì)血脂的改善。4)在減輕體重方面運(yùn)動(dòng)聯(lián)合EGCG和LC組有效疊加了運(yùn)動(dòng)、LC的獨(dú)立效果。
[Abstract]:Objective: To observe the effect of exercise and exercise combined with EGCG or / or L-carnitine on weight loss and to explore its effects on fat metabolism and energy metabolism.
Study methods: 4 weeks old male SD rats were used as the research object, high fat diet induced diet obesity model. Then 6 weeks of weight loss intervention experiment were carried out: obese rats were randomly divided into obesity model group (F, n=6), simple exercise group (S, n=6), exercise combined EGCG group (E, n=6), exercise combined LC group (L, n=6), exercise joint EGCG and concerned, still given High fat diet; exercise with medium and low intensity treadmill exercise, EGCG and LC gavage at 80mg/kg BW dose. After the experiment, blood lipid, liver lipase activity, and epididymal fat HSL and brown fat UCPl protein expression level were tested.
The results were as follows: 1) compared with the F group, the weight of the rats in the intervention group was significantly lower (P0.05): the weight of the EL group was significantly lower than that in the S group (P0.05), and there was no difference between the other groups (P0.05).2) and the serum TG was significantly lower than the F group (P0.01) in the intervention groups (P0.01), and the TC showed a decreasing trend (P0.05). In group EL, TC and HDL-C were significantly improved (P0.05, P0.01), and there was no significant difference between the other groups (P0.05).3). Compared with the F group, the activity of livers was significantly increased in the E group and L group, and the livers activity of the group was significantly higher than that in the group of E. The level of pre group HSL protein was significantly higher than that in group F (P0.01).E group, HSL protein expression was higher than that of S group (P0.05). There was no significant difference between the other groups (P0.05).5). Compared with F group, there was no significant difference between S group and L group.
Conclusions: 1) 1) 6 weeks of exercise or exercise combined with EGCG or (and) LC can reduce the weight of obese rats, improve liver lipase activity, improve blood lipid metabolism, enhance the expression of HSL protein in adipose tissue and promote lipid mobilization and oxidative utilization of.2) EGCG shows the ability to improve the expression of brown fat UCP1 protein, aerobic exercise or LC to BAT No effect on.3) EGCG has a synergistic effect on improving liver lipase, improving blood lipid and enhancing the expression of HSL protein, while LC only synergistically promotes exercise to improve blood lipid.4) the exercise combined with EGCG and LC groups effectively combined exercise and LC's independent effect.
【學(xué)位授予單位】：北京體育大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：G804.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 吉寧;李寧;;茶多酚的健康促進(jìn)作用及分子機(jī)制研究進(jìn)展[J];國(guó)外醫(yī)學(xué)(衛(wèi)生學(xué)分冊(cè));2007年04期

2 耿稚江;;肥胖手術(shù)治療可消除肥胖相關(guān)疾病風(fēng)險(xiǎn)[J];國(guó)外醫(yī)學(xué)情報(bào);2005年04期

3 劉穎;劉曉莉;;肉毒堿在血液透析中的作用[J];現(xiàn)代護(hù)理;2006年16期

4 巫傳玲;;左旋肉堿的應(yīng)用[J];西南軍醫(yī);2010年06期

5 王詠梅,殷仁富,吳宗貴,梅長(zhǎng)林,顧書華,劉皋林,張純;左旋卡尼汀對(duì)老年糖尿病合并高血壓病患者糖脂代謝的影響[J];中華老年心腦血管病雜志;2002年04期

6 范雪;嚴(yán)政;;有機(jī)鉻、L-肉堿及有氧運(yùn)動(dòng)對(duì)高脂飲食大鼠脂肪代謝的影響[J];南京體育學(xué)院學(xué)報(bào)(自然科學(xué)版);2010年01期

7 李麗春,吳曉東,田維熙;何首烏提取物對(duì)脂肪酸合酶的抑制作用[J];中國(guó)生物化學(xué)與分子生物學(xué)報(bào);2003年03期

8 畢業(yè);李建設(shè);;有氧運(yùn)動(dòng)對(duì)單純性肥胖兒童血中瘦素、神經(jīng)肽、胰島素和腫瘤壞死因子的影響研究[J];體育科學(xué);2005年12期

9 趙秀玲;靜蕓蕓;;對(duì)國(guó)家藥品不良反應(yīng)監(jiān)測(cè)中心已發(fā)布36期《藥品不良反應(yīng)信息通報(bào)》的匯總分析[J];齊魯藥事;2011年04期

10 張媛;漆正堂;丁樹哲;;運(yùn)動(dòng)性骨骼肌線粒體脂肪氧化對(duì)脂肪代謝的影響[J];武漢體育學(xué)院學(xué)報(bào);2008年02期

相關(guān)重要報(bào)紙文章 前2條

1 楊六香;[N];中國(guó)醫(yī)藥報(bào);2004年

2 記者 陳靜;[N];中國(guó)醫(yī)藥報(bào);2010年

相關(guān)博士學(xué)位論文 前2條

1 張曉剛;脂蛋白脂酶、肝脂酶與動(dòng)脈粥樣硬化的研究——機(jī)理與防治[D];重慶醫(yī)科大學(xué);2002年

2 田吉明;運(yùn)動(dòng)對(duì)肥胖大鼠脂類代謝調(diào)節(jié)因素的影響[D];北京體育大學(xué);2007年

相關(guān)碩士學(xué)位論文 前3條

1 曹海霞;運(yùn)動(dòng)對(duì)上海市超重和肥胖中老年女性健康的影響[D];上海體育學(xué)院;2010年

2 張忠英;運(yùn)動(dòng)減肥對(duì)肥胖兒童少年脂蛋白脂酶活性和血脂影響的研究[D];上海體育學(xué)院;2010年

3 南曉琴;兒童單純性肥胖癥對(duì)循環(huán)系統(tǒng)損害的研究[D];山西醫(yī)科大學(xué);2003年

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