本文選題：電磁輻射 + 腦��； 參考：《鄭州大學(xué)》2017年碩士論文

【摘要】：1背景和目的隨著電子技術(shù)的應(yīng)用和通訊工業(yè)的迅速發(fā)展,各種電子設(shè)備、無線通訊設(shè)施廣泛應(yīng)用于日常生活和工作中,環(huán)境中的電磁輻射(Electromagnetic radiation)也日漸增多,甚至有人稱其為的第四大環(huán)境公害。處于生長發(fā)育期的兒童同樣不可避免的暴露于各種頻率和強(qiáng)度的電磁輻射中。有關(guān)電磁輻射對中樞神經(jīng)系統(tǒng)功能的影響研究越來越多,但目前對電磁輻射對大腦影響研究主要集中在對成熟腦組織影響上,發(fā)現(xiàn)移動(dòng)電話產(chǎn)生的電磁輻射可導(dǎo)致成年小鼠血腦屏障通透性增加,神經(jīng)遞質(zhì)代謝紊亂,皮層及海馬出現(xiàn)組織學(xué)和超微結(jié)構(gòu)損傷,如神經(jīng)元缺失、細(xì)胞排列紊亂,胞漿尼氏體減少、核內(nèi)出現(xiàn)透明區(qū)等早期凋亡等一系列征象。但對于不成熟腦組織研究較少,有研究表明機(jī)體器官組織在發(fā)育期對電磁場的影響尤其敏感,而其中又以相對不成熟的腦最易受影響,如對記憶、學(xué)習(xí)能力和神經(jīng)行為能力的影響成為國內(nèi)外學(xué)者日益關(guān)注的焦點(diǎn)。由于電磁輻射神經(jīng)生物學(xué)損傷機(jī)制的復(fù)雜性,所以電磁輻射對處于不同發(fā)育成熟度腦組織影響的是否存在差別,目前尚無明確結(jié)論。本研究選用7日齡、21日齡小鼠,從腦發(fā)育成熟度分別相當(dāng)于新生兒和青少年期;通過模擬手機(jī)1800MHz電磁輻射暴露環(huán)境,研究短期電磁輻射暴露對不同發(fā)育成熟度小鼠腦組織海馬區(qū)神經(jīng)細(xì)胞增生的影響,為防治電磁輻射所致發(fā)育期腦損傷提供理論依據(jù)。2材料與方法將7日齡(P7)和21日齡(P21)昆明小鼠隨機(jī)分為輻射組和對照組,每組12只,共48只。實(shí)驗(yàn)通過GTEM Cell(GHz橫電磁波室)模擬手機(jī)輻射環(huán)境,頻率選取1800MHz,場強(qiáng)為28V/m,功率密度為2.0m W/cm2,小鼠置于電磁波室內(nèi),每天8h,一次4小時(shí),一天2次,中間間隔1h,連續(xù)暴露3d。對照組給予虛擬假輻射,輻射組動(dòng)物分別于輻射前1h腹腔注射Brd U50mg/Kg,對照組連續(xù)給予等體積的生理鹽水腹腔注射,連續(xù)腹腔注射3天。各組小鼠在最后一次腹腔注射24h后,經(jīng)無水乙醚吸入麻醉,灌注生理鹽水和甲醛溶液至軀體蒼白變硬,快速分離并取出腦組織,制備海馬層面腦組織切片,采用免疫組織化學(xué)法檢測海馬齒狀回(dentate gyrus,DG)顆粒細(xì)胞下層Caspase-3、Brd U、Ki67、BLBP、HH3的表達(dá)。3結(jié)果3.1電磁輻射對不同日齡小鼠海馬齒狀回顆粒下層(SGZ)神經(jīng)細(xì)胞Caspase-3表達(dá)的影響普通光學(xué)顯微鏡200倍時(shí)鏡下觀察發(fā)現(xiàn):P7及P21小鼠短期暴露于1800MHz電磁輻射后海馬齒狀回顆粒下層幾乎沒有發(fā)現(xiàn)Caspase-3陽性細(xì)胞表達(dá)。與對照組相比,輻射組海馬齒狀回區(qū)結(jié)構(gòu)疏松,神經(jīng)細(xì)胞排列松散、紊亂。3.2電磁輻射對不同日齡小鼠腦組織海馬齒狀回SGZ區(qū)Brd U、Ki67表達(dá)的影響與對照組相比,P7輻射組小鼠腦組織海馬齒狀回SGZ區(qū)Brd U、Ki67陽性細(xì)胞表達(dá)明顯增多,差異有統(tǒng)計(jì)學(xué)意義(P0.05);P21輻射組小鼠與對照組相比,海馬齒狀回SGZ區(qū)Brd U、Ki67陽性細(xì)胞表達(dá)幾乎無變化,差異無統(tǒng)計(jì)學(xué)意義(P0.05)。3.3電磁輻射對不同日齡小鼠腦組織海馬齒狀回SGZ區(qū)BLBP、HH3表達(dá)的影響P7小鼠暴露于電磁輻射后BLBP和HH3的陽性細(xì)胞表達(dá)減少,差異有統(tǒng)計(jì)學(xué)意義(P0.05);而P21輻射組小鼠與對照組相比,BLBP和HH3陽性細(xì)胞表達(dá)幾乎無變化。4結(jié)論1800MHz電磁輻射對不同成熟度小鼠腦組織海馬DG區(qū)神經(jīng)細(xì)胞均未引起凋亡,對發(fā)育相對不成熟腦組織有促進(jìn)神經(jīng)細(xì)胞增生作用,但對發(fā)育相對成熟的腦組織卻無明顯影響。
[Abstract]:1 background and purpose with the application of electronic technology and the rapid development of communication industry, all kinds of electronic equipment and wireless communication facilities are widely used in daily life and work. The electromagnetic radiation (Electromagnetic radiation) in the environment is increasing, and even the fourth environmental hazards, which are called it, are in the same growing period of children. The influence of electromagnetic radiation on the function of central nervous system is more and more, but the influence of electromagnetic radiation on the brain is mainly focused on the mature brain tissue, and it is found that the electromagnetic radiation produced by mobile phone can lead to the blood brain screen of adult mice. An increase in barrier permeability, disorder of neurotransmitter metabolism, histology and ultrastructural damage in the cortex and hippocampus, such as neuron loss, disorder of cell arrangement, cytosolic Nissl body reduction, early apoptosis in the nucleus, and so on. However, there are few studies on immature brain tissue. The influence of electromagnetic field is especially sensitive, and the relatively immature brain is most susceptible to the influence of the brain, such as memory, learning ability and neurobehavioral ability, which has become the focus of attention of scholars at home and abroad. The electromagnetic radiation is in the shadow of different mature brain tissues because of the complexity of the mechanism of neurobiological damage. In this study, 7 days of age and 21 day old mice were selected as the equivalent of the newborn and adolescence in the 7 days of age and 21 days of age. By simulating the exposure environment of the electromagnetic radiation of the mobile phone, the short-term electromagnetic radiation exposure was used to study the proliferation of neurons in the hippocampus of the brain tissues of different mature mice. In order to provide theoretical basis for the prevention and control of brain damage induced by electromagnetic radiation,.2 materials and methods were used to randomly divide the 7 days old (P7) and 21 day old (P21) Kunming mice into radiation and control groups, with 12 rats in each group, with a total of 48. The experiments were carried out through the GTEM Cell (GHz transverse electromagnetic wave chamber) to simulate the radiation environment of the cell phone, the frequency selected 1800MHz, the field strength 28V/m, and the power density. For 2.0m W/cm2, the mice were placed in the electromagnetic wave room, 8h every day, 4 hours a day, 2 times a day, and 1H in the middle interval. The 3D. control group was continuously exposed to virtual false radiation. The radiation group was injected with Brd U50mg/Kg before the radiation of 1H, and the control group was given the equal volume of physiological salt water intraperitoneally for 3 days. The mice in each group were at the end. After an intraperitoneal injection of 24h, inhalation of anhydrous ether, perfusion of saline and Formaldehyde Solution to the body and hard, quickly separating and removing brain tissue, preparing hippocampal slice of brain tissue, using immunohistochemical method to detect Caspase-3, Brd U, Ki67, BLBP, HH3.3 of the dentate gyrus, DG granulosa cells Results the effect of 3.1 electromagnetic radiation on the expression of Caspase-3 expression in the dentate gyrus sublayer (SGZ) neurons of different days of age of mice was 200 times more than that of the ordinary optical microscope. It was found that the P7 and P21 mice were exposed to the 1800MHz electromagnetic radiation of the Houhai dentate gyrus and the Caspase-3 positive cells were hardly found in the 1800MHz electromagnetic radiation. Compared with the control group, the effects of.3.2 electromagnetic radiation on the expression of Brd U and Ki67 in the SGZ region of hippocampus dentate gyrus of mice with different days of age were compared with those of the control group. The expression of Brd U and Ki67 positive cells in the hippocampal dentate gyrus of the mice of P7 radiation group increased significantly, and the difference was statistically significant (the difference was statistically significant). The expression of Brd U and Ki67 positive cells in SGZ area of hippocampal dentate gyrus was almost unchanged, and there was no statistical difference (P0.05).3.3 electromagnetic radiation on BLBP and HH3 expression in the SGZ region of hippocampal dentate gyrus of mice with different days of age (P0.05), and the expression of positive cells decreased after exposure to electromagnetic radiation in P21 radiation group (P0.05). The difference was statistically significant (P0.05), while the P21 radiation group was compared with the control group, the expression of BLBP and HH3 positive cells almost did not change.4 conclusion 1800MHz electromagnetic radiation did not induce apoptosis in the hippocampal DG region nerve cells in the brain tissues of the mice with different maturity. The relatively mature brain tissue has no obvious effect.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R818

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