本文選題：腎素-血管緊張素-醛固酮系統(tǒng) + 有氧運動能力��； 參考：《北京體育大學(xué)》2014年博士論文

【摘要】：目的：通過分析血壓調(diào)節(jié)系列蛋白基因在運動員和普通人中的分布特征及基因多態(tài)性與有氧功能表型指標(biāo)的關(guān)聯(lián)性,篩選有氧運動能力分子標(biāo)記；通過陽性標(biāo)記功能分析,探尋分子調(diào)控機制。 方法： 應(yīng)用MALDI-TOF解析我國優(yōu)秀耐力運動員和普通對照人群AGT、ACE等12個基因22個SNP位點。應(yīng)用SPSS、SHEsis及haplo. stats軟件分析SNP及單體型在各組分布特征,及其與有氧功能表型指標(biāo)關(guān)聯(lián)性。構(gòu)建陽性標(biāo)記和pcDNA3.1重組質(zhì)粒,轉(zhuǎn)染293T細(xì)胞,檢測mRNA表達(dá)。 結(jié)果： (1) AGTR1基因rs5182位點基因型分布頻率在運動員組和對照組間、國際健將組和對照組間、5km運動員組和對照組間以及l(fā)Okm運動員組和對照組間均具有顯著性差異,但轉(zhuǎn)染細(xì)胞培養(yǎng)48小時后,各基因型間mRNA表達(dá)量不具有顯著性差異。(2)rs4961、rs16843523等位基因及TT單體型分布頻率在lOkm運動員組和對照組間具有顯著性差異；CYP11B1、CYP11B2基因單體型分布頻率在國際健將組和對照組間、馬拉松組和對照組間以及女運動員組和女對照組間具有顯著性差異。由于樣本量較小,上述位點能否作為選材用分子標(biāo)記有待確認(rèn)。(3) rs2148582、rs5707、rs11240688、rs4344、rs4343、rs5182、rs1403543,及REN、CYP11B1和CYP11B2(1-3)、ADD1單體型與肺功能指標(biāo)顯著關(guān)聯(lián)。rs2148582、rs11240688、rs4344、rs4363、rs4343、rs6810951、rs11737660、rs7121,及REN、ACE、CYP11B1-CYP11B2單體型與心血管功能指標(biāo)顯著關(guān)聯(lián)。 結(jié)論： AGTR1基因rs5182位點可以作為我國北方漢族有氧耐力運動員選材用分子標(biāo)記,但該位點并非通過AT1R蛋白及mRNA表達(dá)量調(diào)控運動能力。rs2148582、rs5707、 rs11240688、rs4344、rs4343、rs5182、rs4363、rs6810951、rs11737660、 rs7121、rs1403543,以及REN、ACE、CYP11B1-CYP11B2、ADD1單體型可以作為預(yù)測我國北方漢族優(yōu)秀有氧耐力運動員有氧功能表型指標(biāo)的候選分子指標(biāo)。
[Abstract]:Objective: to screen molecular markers of aerobic exercise ability by analyzing the distribution characteristics of blood pressure regulation series protein genes in athletes and ordinary people, and the association between gene polymorphism and aerobic functional surface index, and to analyze the function of positive markers. To explore the mechanism of molecular regulation. Methods: MALDI-TOF was used to analyze 22 SNP loci of 12 genes such as AGTN ACE in Chinese elite endurance athletes and general control population. The distribution characteristics of SNP and haplotype in each group were analyzed by SPSS SHEsis and haplo. stats software. The positive labeled and pcDNA3.1 recombinant plasmids were constructed and transfected into 293T cells to detect the expression of mRNA. Results: 1) there were significant differences in the distribution frequency of AGTR1 gene rs5182 genotype between athletes and control groups, between international athletes and control groups, and between lOkm athletes and control groups. But after 48 hours of culture, There was no significant difference in mRNA expression among different genotypes. The allele frequency of rs4961rs16843523 and the frequency of TT haplotype distribution were significantly different between the lOkm athletes and the control group. The distribution frequency of CYP11B1CY CYP11B2 haplotype was found between the international athletes and the control group. There were significant differences between the marathon group and the control group and between the female athletes group and the female control group. 鐢變簬鏍鋒湰閲忚緝?yōu)?

本文編號：1959853