本文選題：黃精多糖 + 大強度運動�。� 參考：《成都體育學院》2012年碩士論文

【摘要】：目的：觀察黃精多糖對大強度運動所致人體T淋巴細胞免疫功能低下的影響，分析其產(chǎn)生影響的可能機制，探討減輕運動性免疫功能低下的新手段。方法：體育學院無系統(tǒng)專業(yè)運動訓練經(jīng)歷男生14名，隨機分為安慰劑組（A組）和黃精多糖組（B組），每組7人。A、B兩組受試者按1g/d劑量分別口服安慰劑和黃精多糖4周后，進行一次大強度遞增負荷跑臺運動，于運動前、運動后即刻、運動后24h取受試者肘正中靜脈血各10ml，MTT法檢測外周血T淋巴細胞轉化指數(shù)（T-LTI），流式細胞術檢測T淋巴細胞亞群（CD4~+、CD8~+）百分比，雙抗體夾心ABC—ELISA法檢測Th1、Th2型細胞因子（IFN-γ、IL-4）、放射免疫法檢測血漿皮質醇（Cor）。結果：1.A、B兩組受試者運動前各指標無顯著性差異。2.兩組受試者T-LTI運動后即刻均低于運動前，其中A組有非常顯著性差異；運動后24h，兩組T-LTI均有回升，A組接近運動前水平，B組稍高于運動前水平；B組運動后兩時相T-LTI均高于A組對應時相，運動后即刻有顯著性差異。3.A組運動后即刻CD8~+明顯高于運動前，CD4~+、CD4~+/CD8~+明顯低于運動前，運動后24h各指標有所恢復，但未達到運動前水平，CD4~+/CD8~+仍明顯低于運動前；B組運動后兩時相各指標變化趨勢與A組相同，但變化幅度均明顯小于A組，與運動前比較均無顯著性差異，，CD4~+、CD4~+/CD8~+在運動后24h恢復到略高于運動前水平。4.兩組受試者運動后即刻IFN-γ均低于運動前，其中A組有顯著性差異，B組運動后即刻IFN-γ明顯高于A組，運動后24h，兩組IFN-γ均回升到接近運動前水平；兩組IL-4運動后即刻均非常顯著高于運動前，運動后24h有下降，但仍高于運動前水平，其中A組有顯著性差異；兩組IFN-γ/IL-4運動后即刻非常顯著低于運動前，運動后24h有回升，但仍低于運動前，其中A組有顯著性差異。5.A組Cor運動后即刻明顯高于運動前，運動后24h仍高于運動前，但無顯著性差異；B組Cor運動后即刻和運動后24h都低于運動前水平，但無顯著性差異。結論：一次大強度跑臺運動導致人體T淋巴細胞免疫功能受到抑制,預先補充黃精多糖能減輕運動導致的T淋巴細胞增殖能力的下降、CD4~+細胞含量和IFN-γ水平的降低以及CD8~+細胞含量和IL-4水平的升高，進而預防CD4~+/CD8~+比值、IFN-γ/IL-4比值的下降，表明黃精多糖能有效降低運動導致的機體免疫平衡趨向抑制的程度，減輕免疫穩(wěn)態(tài)向體液免疫偏移的程度。其作用機制可能與黃精多糖能通過抑制運動后血漿皮質醇水平的升高，從而達到減輕其對機體免疫功能的負調節(jié)作用有關。
[Abstract]:Aim: to observe the effect of Polygonatum flavescentis polysaccharides on the immune function of human T lymphocytes induced by high intensity exercise, analyze the possible mechanism of its influence, and explore a new method to alleviate the low immune function caused by exercise. Methods: fourteen male students undergoing sports training without systematic major in physical education college were randomly divided into placebo group (group A) and flavin polysaccharide group (group B). The subjects of group A and group B were given placebo and flavin polysaccharides for 4 weeks according to the dose of 1g/d, respectively. A large intensity incremental load treadmill movement, before the exercise, immediately after the exercise, 24 hours after exercise, T lymphocyte transformation index (T-LTI) and T lymphocyte subsets (CD4 ~ + CD8 ~) percentage were measured by MTT assay and flow cytometry respectively. Double antibody sandwich ABC-ELISA assay was used to detect Th1Th 2 type cytokine, and radioimmunoassay was used to detect plasma cortisol. Results 1. There was no significant difference between the two groups before exercise. The T-LTI of the two groups was lower immediately after exercise than that before exercise, and there was a very significant difference between group A and group A. At 24 hours after exercise, the T-LTI of group A was higher than that of group B at 02:00 after exercise, and the T-LTI of group B was higher than that of group A. There was significant difference immediately after exercise. 3. In group A, immediately after exercise, CD8- ~ was significantly higher than that before exercise, CD4- / -CD8- was significantly lower than that before exercise, and the indexes recovered 24 hours after exercise. However, the change trend of each index in group B after exercise was the same as that in group A, but the change range was obviously smaller than that in group A. There was no significant difference between before exercise and before exercise. CD4 ~ / CD4 ~ / CD8 ~ was slightly higher than that of pre-exercise at 24 h after exercise, and the level of CD4 ~ / CD8 ~ was slightly higher than that before exercise. IFN- 緯 in group A was significantly higher than that in group A immediately after exercise, and IFN- 緯 in group B was significantly higher than that in group A immediately after exercise. At 24 hours after exercise, IFN- 緯 in both groups increased to close to the pre-exercise level. Immediately after exercise, IL-4 in both groups was significantly higher than that before exercise, decreased 24 hours after exercise, but still higher than that before exercise, and there was a significant difference in group A, IFN- 緯 / IL-4 immediately after exercise was significantly lower than that before exercise, and there was a rise 24 hours after exercise. The Cor of group A was significantly higher than that of before exercise and 24 hours after exercise. However, there was no significant difference between group B and group B in Cor immediately after exercise and 24 hours after exercise, but the level of Cor in group B was lower than that in group B immediately after exercise and 24 hours after exercise. But there was no significant difference. Conclusion: the immune function of human T lymphocytes was inhibited by a strong treadmill exercise. Supplementation of flavin polysaccharides in advance could attenuate the decrease of proliferation ability of T lymphocytes induced by exercise and the decrease of CD4- 緯 and IFN- 緯 levels, as well as the increase of CD8- cells and IL-4 levels, thereby preventing the decrease of CD4 ~ / CD8- 緯 / IL-4 ratio. The results showed that the polysaccharide could effectively reduce the degree of inhibition of immune balance and the shift of immune homeostasis to humoral immunity. The mechanism may be related to the inhibition of the increase of plasma cortisol after exercise, which can alleviate the negative regulation of the immune function of the body.
【學位授予單位】：成都體育學院
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：G804.2;R285.5

【參考文獻】

相關期刊論文 前10條

1 毛雁;韓曉燕;熊正英;劉曉軍;;黃精提取物對耐力訓練大鼠骨骼肌組織NO-NOS體系影響的實驗研究[J];北京體育大學學報;2008年09期

2 睢大員,于曉鳳,呂忠智,高普軍,樸云峰,宋國培;枸杞子、北五味子和黃精三種粗多糖的增強免疫與抗脂質過氧化作用[J];白求恩醫(yī)科大學學報;1996年06期

3 曾慶華;余曉琳;廖品正;蒙義文;莫衛(wèi)平;陳素文;;黃精多糖制劑治療家兔單純皰疹病毒性角膜炎的實驗觀察[J];成都中醫(yī)學院學報;1988年01期

4 王紅玲,張渝侯,洪艷,劉君炎;黃精多糖對哮喘患兒紅細胞免疫功能影響的體外實驗研究[J];中國當代兒科雜志;2002年03期

5 毛雁;馬蘭軍;熊正英;;黃精對力竭訓練大鼠血清酶活性及某些生化指標的影響[J];第四軍醫(yī)大學學報;2007年20期

6 王紅玲,張渝侯,洪艷,劉君炎;黃精多糖對小鼠血糖水平的影響及機理初探[J];兒科藥學;2002年01期

7 白潤江,任婭,于紅娟;三種中藥多糖元素含量測定及與免疫關系研究結果[J];甘肅中醫(yī)學院學報;1997年04期

8 李寧川,金其貫,顏軍;不同方式的力竭運動對大鼠細胞免疫機能的影響[J];廣州體育學院學報;2000年02期

9 李友元,鄧洪波,王蓉,陳瓏瓏;衰老小鼠組織端粒酶活性變化及黃精多糖的干預作用[J];醫(yī)學臨床研究;2005年07期

10 李友元,張萍,鄧洪波,陳瓏瓏;動脈粥樣硬化家兔VCAM-1表達及黃精多糖對其表達的影響[J];醫(yī)學臨床研究;2005年09期

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