本文選題：運(yùn)動(dòng)性骨骼肌損傷 + 損傷后修復(fù)��； 參考：《吉林大學(xué)》2012年碩士論文

【摘要】：研究目的：探索大鼠經(jīng)不同負(fù)荷（無負(fù)荷、中等強(qiáng)度、大強(qiáng)度）運(yùn)動(dòng)訓(xùn)練后，誘導(dǎo)其骨骼肌損傷，在自然修復(fù)期和藥物（云南白藥）治療期血液中肌酸激酶（CK）和乳酸脫氫酶（LDH）的變化，為骨骼肌損傷的預(yù)防、損傷后的準(zhǔn)確診斷及其治療提供理論依據(jù)，為科學(xué)的體育鍛煉提供理論基礎(chǔ)。 研究方法：對(duì)經(jīng)過為期8周的訓(xùn)練的中等強(qiáng)度(Z)組、大強(qiáng)度(D)組和無負(fù)荷組的大鼠，建立大鼠運(yùn)動(dòng)損傷模型（參照田野運(yùn)動(dòng)訓(xùn)練損傷模型的建立方法）誘導(dǎo)其骨骼肌損傷。將每組大鼠隨機(jī)為治療組及非治療組，治療組從24小時(shí)至第10天給予噴灑云南白藥氣霧劑，非治療組給予噴灑等劑量的生理鹽水。上述三組大鼠治療組和非治療組損傷后按即刻、1天、4天、7天、10天，5個(gè)時(shí)間段取血測(cè)定血清肌酸激酶（CK）及乳酸脫氫酶（LDH）活性的變化。 研究結(jié)果： 1、大負(fù)荷非治療訓(xùn)練組大鼠的在損傷后即刻和修復(fù)期的1、4、7、10天CK值和LDH值較安靜對(duì)照組高（P0.05）。與中等負(fù)荷非治療組相比，大負(fù)荷非治療組CK值和LDH值在損傷后即刻、損傷后第1天和第4天均較高（P0.05）；而在損傷后的第7天至10天大負(fù)荷組CK值和LDH值較低，但二者無顯著性差異（P0.05）；與無負(fù)荷非治療組相比，大負(fù)荷非治療訓(xùn)練組的大鼠CK、LDH值在損傷后即刻較高，但他們之間無顯著性差異（P0.05）；CK值和LDH值在損傷修復(fù)期的第4天較高（P0.05）。 2、大負(fù)荷治療組大鼠在骨骼肌損傷后的第4天至10天CK值低于大負(fù)荷非治療組(p0.05)；而LDH值則在第4天低于大負(fù)荷非治療組(p0.05)。與中等負(fù)荷治療組和無負(fù)荷治療組相比，大負(fù)荷治療組CK值和LDH值在損傷后4天時(shí)較高(p0.05)。 3、中等負(fù)荷非治療組在骨骼肌損傷后即刻、損傷后的第1天、第4天血清CK值和LDH值高于安靜對(duì)照組（P0.05）。損傷后的第7天至10天血清CK值和LDH值已接近安靜對(duì)照組CK值，二者之間無顯著性差異（P0.05）。 4、無負(fù)荷非治療組骨骼肌損傷后即刻和損傷后的恢復(fù)期的第1天、4天、7天血清CK值高于安靜對(duì)照組（P0.05），在損傷后的第10天CK酶值接近于安靜對(duì)照組；而血清LDH則在骨骼肌損傷后即刻和損傷后的第1天、4天高于安靜對(duì)照組（P0.05）。與中等負(fù)荷非治療訓(xùn)練組相比，無負(fù)荷組大鼠非治療組CK值和LDH值在損傷后即刻較高(p0.05)；損傷后的第1天至第10天該值雖有升高但無顯著性差異（P0.05）。 5、無負(fù)荷治療組在骨骼肌損傷后的第4天至10天CK值和LDH值均低于大負(fù)荷組大鼠非治療組(p0.05)；高于損傷后的第4天的中等強(qiáng)度治療訓(xùn)練組，但無顯著性差異（P0.05）；7天至10天無負(fù)荷組CK值和LDH值接近于安靜對(duì)照組。 結(jié)論： 1．根據(jù)中等負(fù)荷非治療組大鼠CK和LDH值較大負(fù)荷和無負(fù)荷訓(xùn)練非治療組大鼠在損傷后即刻和修復(fù)期的第1天、4天低（P0.05），第7天和第10天無顯著性差異的特征，表明中等負(fù)荷組大鼠損傷程度比大負(fù)荷和無負(fù)荷訓(xùn)練組輕，并且自然修復(fù)快。說明中等負(fù)荷組大鼠骨骼肌的抗損傷能力高于大負(fù)荷組和無負(fù)荷組。 2．根據(jù)大負(fù)荷訓(xùn)練的大鼠CK、LDH值在損傷后即刻較無負(fù)荷訓(xùn)練的大鼠高，但他們之間無顯著性差異；在損傷后的4天至10天較無負(fù)荷訓(xùn)練的大鼠CK、LDH值要高（P0.01）的特征，我們可以認(rèn)為大負(fù)荷訓(xùn)練組與無負(fù)荷組在損傷即刻其損傷程度沒有統(tǒng)計(jì)學(xué)差異，但大負(fù)荷訓(xùn)練組在損傷4天以后自然修復(fù)速度較無負(fù)荷訓(xùn)練組要慢。表明大負(fù)荷組在8周的大負(fù)荷訓(xùn)練和誘導(dǎo)骨骼肌損傷過程中可能有重復(fù)性損傷，說明骨骼肌損傷具有積累效應(yīng)。 3．根據(jù)大負(fù)荷組、中等負(fù)荷組和無負(fù)荷運(yùn)動(dòng)組大鼠骨骼肌損傷后經(jīng)過云南白藥治療后的第4天、第7天、第10天CK酶和LDH酶均低于非治療組(P0.05)的特征。說明云南白藥能加速大鼠運(yùn)動(dòng)性骨骼肌損傷的修復(fù)。
[Abstract]:Objective: To explore the injury of skeletal muscle in rats after exercise training with different loads (no load, medium intensity and intensity). The changes of creatine kinase (CK) and lactate dehydrogenase (LDH) in the natural repair period and drug (Yunnan Baiyao) treatment period are the prevention of skeletal muscle injury, the accurate diagnosis and treatment of the injury after injury. On the basis of this, it provides a theoretical basis for scientific physical training.
Methods: the rats in the medium intensity (Z) group, the high intensity (D) group and the non load group were trained to induce the injury of the skeletal muscle in the rats (reference field training damage model). The rats in each group were randomly assigned to the treatment group and the non treatment group. The treatment group was given from 24 to tenth days. The non treatment group was sprayed with the spray of Yunnan Baiyao aerosol, and the non treatment group was sprinkled with equal dose of saline. The changes of serum creatine kinase (CK) and lactate dehydrogenase (LDH) activity were measured in the three groups of rats and non treatment groups at immediate, 1, 4, 7, 10 and 5 time periods.
The results of the study:
1, the CK value and LDH value of 1,4,7,10 days in the immediate and repair stage of the large load non treatment training group were higher than those in the quiet control group (P0.05). Compared with the medium load non treatment group, the CK value and LDH value of the non treatment group of large load were higher immediately after the injury and the first and fourth days after injury (P0.05), while the seventh to 10 days after the injury were large. The CK value and LDH value of the group were low, but there was no significant difference between the two (P0.05). Compared with the non load non treatment group, the LDH value of the rats in the non treatment training group was higher, but there was no significant difference between them (P0.05), and the CK value and LDH value were higher at the fourth day of the injury repair period (P0.05).
2, the CK value of the large load treatment group was lower than the non treatment group (P0.05) in the fourth day to 10 day after the skeletal muscle injury, while the LDH value was lower than the non treatment group (P0.05) at fourth days. Compared with the medium load treatment group and the non load treatment group, the CK value and the LDH value were higher at 4 days after the injury (P0.05).
3, the serum CK and LDH values were higher than those in the quiet control group (P0.05) on the first day after injury, and the serum CK and LDH values after the injury were close to the CK value of the quiet control group after the injury, and there was no significant difference between the two groups (P0.05).
4, the serum CK value was higher than that in the quiet control group (P0.05) on the 4 day and 7 days after the skeletal muscle injury in the non load non treatment group, and the CK enzyme value was close to the quiet control group on the tenth day after the injury, while the serum LDH was higher than that in the quiet control group (P0.05) after the skeletal muscle injury immediately and after the injury, and 4 days after the injury. The serum LDH was higher than the medium negative group (P0.05). The CK and LDH values in the non treatment group of the non load group were higher than those in the non load group (P0.05), and there was no significant difference between the first days and the tenth days after the injury (P0.05).
5, the CK value and LDH value of the fourth day to 10 day after skeletal muscle injury in the non load treatment group were lower than the non treatment group (P0.05) in the large load group, and higher than the medium intensity treatment training group for fourth days after the injury, but there was no significant difference (P0.05), and the CK value and LDH value of the non load group from 7 to 10 days were close to the quiet control group.
Conclusion:
1. according to the medium load non treatment group, the rats in the non treatment group had CK and LDH load and no load training in the non treatment group. There was no significant difference between the 4 days (P0.05), seventh days and tenth days after injury. It showed that the injury degree of the rats in the medium load group was lighter than the heavy load and the non load training group, and the natural repair was faster. It indicates that the injury resistance of skeletal muscle in middle load group is higher than that in high load group and no load group.
2. the LDH value of rat CK based on heavy load training was higher than that of the non load training rats immediately after injury, but there was no significant difference between them. In the 4 to 10 days after injury, the CK of the rats without load training was higher (P0.01), and we could think that the damage degree of the heavy load training group and the non load group was not immediately damaged. There were statistical differences, but the rate of natural repair in the large load training group was slower than that in the non load training group after 4 days of injury. It showed that the heavy load group could have repeated injury during the 8 week heavy load training and the induction of skeletal muscle injury, indicating the accumulation effect of skeletal muscle injury.
3. according to the large load group, the CK enzyme and LDH enzyme were lower than the non treatment group (P0.05) on the fourth day after the treatment of the skeletal muscle injury of the rats in the medium load group and the non load exercise group after the treatment of the Yunnan Baiyao, and the tenth day was lower than that of the non treatment group. It showed that the white medicine can accelerate the repair of the motor skeletal muscle injury in the rats.

【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R87

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本文編號(hào)：1881599