本文選題：氫 + 運(yùn)動(dòng)過(guò)度　； 參考：《中國(guó)組織工程研究》2015年29期

【摘要】：背景:富氫水能否用于離心運(yùn)動(dòng)肌損傷的防護(hù)及其相關(guān)機(jī)制尚不清楚。目的:觀察富氫水對(duì)離心運(yùn)動(dòng)大鼠骨骼肌線粒體氧化應(yīng)激及炎癥反應(yīng)的影響,并探討富氫水作用的相關(guān)信號(hào)通路。方法:將40只SD大鼠隨機(jī)分為對(duì)照組、離心運(yùn)動(dòng)組、離心運(yùn)動(dòng)+生理鹽水組和離心運(yùn)動(dòng)+富氫水組。后3組大鼠連續(xù)5d進(jìn)行下坡跑,跑臺(tái)速度為16-18m/min,坡度-16°,90min/d,共5d。離心運(yùn)動(dòng)+富氫水組大鼠在每次運(yùn)動(dòng)后即刻腹腔注射飽和富氫水(10mL/kg);離心運(yùn)動(dòng)+生理鹽水組大鼠注射生理鹽水。結(jié)果與結(jié)論:離心運(yùn)動(dòng)同時(shí)給予富氫水顯著上調(diào)Sirtuin-3(Sirt3)表達(dá),提高線粒體膜電位和錳超氧化物歧化酶活性,抑制線粒體活性氧生成和線粒體DNA氧化損傷,降低炎性因子NOD樣受體熱蛋白結(jié)構(gòu)域相關(guān)蛋白3和白細(xì)胞介素1β表達(dá)。表明富氫水除可直接清除活性氧外,還可通過(guò)上調(diào)SIRT3表達(dá),提高線粒體能量代謝和抗氧化能力,從而抑制離心運(yùn)動(dòng)誘導(dǎo)的線粒體氧化應(yīng)激和繼發(fā)的炎癥反應(yīng),實(shí)現(xiàn)骨骼肌保護(hù)效應(yīng)。
[Abstract]:Background: it is unclear whether hydrogen-rich water can be used in the protection of eccentric muscle injury and its related mechanism. Aim: to investigate the effects of hydrogen rich water on oxidative stress and inflammatory response of skeletal muscle mitochondria during centrifugation in rats, and to explore the signal pathways related to the action of hydrogen rich water. Methods: forty Sprague-Dawley rats were randomly divided into control group, centrifuge exercise group, centrifuge normal saline group and hydrogen rich water group. The latter three groups were followed by downhill running for 5 days. The running speed was 16-18 m / min, and the slope was -16 擄/ 90 min / d for 5 days. Rats in the group of hydrogen-rich water in centrifugation group were injected with saturated hydrogen rich water 10 mL / kg immediately after each exercise, and rats in the group of normal saline in centrifugal exercise group were injected with normal saline. Results & Conclusion: at the same time, the hydrogen-rich water increased the expression of Sirtuin-3n Sirt3, increased the mitochondrial membrane potential and the activity of manganese superoxide dismutase, inhibited the production of reactive oxygen species in mitochondria and oxidative damage of mitochondrial DNA. The expression of inflammatory factor NOD like receptor domain associated protein 3 and interleukin 1 尾 was decreased. In addition to scavenging reactive oxygen species directly, hydrogen-rich water can also increase the energy metabolism and antioxidant capacity of mitochondria by upregulating the expression of SIRT3, thus inhibiting the oxidative stress and the secondary inflammatory response induced by centrifugal exercise. The protective effect of skeletal muscle was realized.
【作者單位】： 上海工程技術(shù)大學(xué)體育部;武警后勤學(xué)院附屬醫(yī)院救援醫(yī)學(xué)研究所;武警后勤學(xué)院附屬醫(yī)院胸外科;
【基金】：全軍重點(diǎn)實(shí)驗(yàn)室開(kāi)放基金(JY1406)~~
【分類號(hào)】：R873

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