本文選題：鈷60-γ + 支持細(xì)胞�。� 參考：《鄭州大學(xué)》2013年碩士論文

【摘要】：研究背景及目的：隨著工業(yè)化進(jìn)程的加速,日常生活中環(huán)境輻射日益加劇,人們對(duì)放射性損傷也越來越重視。放射線可損傷全身多個(gè)系統(tǒng),其中性腺組織對(duì)放射線極為敏感。支持細(xì)胞(Sertoli cells)作為睪丸生精上皮中唯一的非生殖細(xì)胞,是維持生精小管內(nèi)生精環(huán)境穩(wěn)定的重要細(xì)胞。因而,任何因素導(dǎo)致睪丸支持細(xì)胞結(jié)構(gòu)及功能的損傷都有可能影響精子的形成,甚至導(dǎo)致男性不育。本課題擬通過不同劑量的鈷60(60Co)-γ射線局部照射小鼠睪丸,并觀察相應(yīng)指標(biāo)的變化,以明確不同劑量60Co-γ射線致小鼠睪丸支持細(xì)胞結(jié)構(gòu)及功能急性損傷的程度,并初步探討引起這種損傷的機(jī)制。 方法：48只健康雄性KM小鼠,采用隨機(jī)數(shù)字表法平均分為4組(n=12)：正常對(duì)照組(A組)；60Co-γ射線雙側(cè)睪丸局部照射組有3個(gè)劑量組：劑量2Gy(B組)；劑量6Gy(C組)；劑量10Gy(D組),局部均勻照射下腹部,時(shí)間設(shè)定為517秒。照射完成48h后,處死小鼠,測(cè)定實(shí)驗(yàn)小鼠身體及雙睪丸重量并采集血液3ml；采用酶聯(lián)免疫吸附法(ELISA)檢測(cè)血清中生殖激素,即：促黃體生成素(luteinizing hormone, LH)、卵泡刺激素(follicle-stimulating hormone, FSH)以及抑制素β (Inhibin0)水平；應(yīng)用光學(xué)顯微鏡觀察小鼠睪丸組織形態(tài)學(xué)變化；應(yīng)用實(shí)時(shí)熒光定量PCR法(Real-time PCR)檢測(cè)實(shí)驗(yàn)小鼠睪丸組織中抑制素β mRNA的相對(duì)表達(dá)量；采用酶聯(lián)免疫吸附法(ELISA)檢測(cè)實(shí)驗(yàn)小鼠睪丸組織中抗氧化指標(biāo),即：還原性谷胱甘肽(Glutathione, GSH)、超氧化物歧化酶(Superoxide dismutase, SOD)、丙二醛(Malondialdehyde, MDA)含量。 結(jié)果：60Co-γ射線照射組(B、C、D組)較正常組(A組)睪丸重量下降(P0.05),以C、D兩組更為明顯(P0.01)；血清生殖激素FSH水平明顯上升(P0.05),而抑制素p水平明顯下降(P0.05)；光鏡下可見小鼠睪丸生精細(xì)胞及支持細(xì)胞均發(fā)生不同程度的損傷,其中以D組損傷程度最為明顯；睪丸組織抑制素βmRNA表達(dá)水平明顯下降,差異均有統(tǒng)計(jì)學(xué)意義(allP0.05);睪丸組織SOD含量呈下降趨勢(shì)(P0.05),其中C、D兩組下降較為明顯(allP0.05);GSH含量亦呈下降趨勢(shì),差異均有統(tǒng)計(jì)學(xué)意義(allP0.05), MDA含量呈上升趨勢(shì),差異均有統(tǒng)計(jì)學(xué)意義(allP0.05)。 結(jié)論：60Co-γ射線對(duì)睪丸支持細(xì)胞結(jié)構(gòu)及功能有明顯損傷作用,且損傷程度與照射劑量呈正相關(guān)性；損傷的機(jī)制與射線致睪丸抗氧化系統(tǒng)受損,引起睪丸組織氧化-抗氧化系統(tǒng)失衡導(dǎo)致氧化應(yīng)激損傷作用有關(guān)。
[Abstract]:Background and purpose: with the acceleration of industrialization, the environmental radiation in daily life is getting more and more serious, and people pay more and more attention to the radiation damage. Radiation can damage multiple systems throughout the body, in which gonadal tissue is extremely sensitive to radiation. Sertoli cells, as the only non-germ cells in spermatogenic epithelium of testis, are important cells to maintain stable spermatogenic environment in seminiferous tubules. Therefore, any cause of testicular Sertoli cell structure and function damage may affect the formation of sperm, and even lead to male infertility. In order to determine the degree of acute injury of testicular Sertoli cell structure and function induced by different doses of 60Co- 緯 ray, the mice testis were irradiated locally with different doses of cobalt 60Co- 緯 ray and the changes of corresponding indexes were observed. The mechanism of the injury was also discussed. Methods A total of 48 healthy male km mice were used. The method of random digital table was used to divide into 4 groups on average: normal control group, group A, group A, group A: dose 2Gy(B group, dose group 6Gy(C group, dose group 10Gy(D group, local uniform irradiation of lower abdomen, time was set at 517 seconds. After 48 hours of irradiation, the mice were killed, the weight of the body and testis of the experimental mice were measured, and the blood was collected 3 ml. The serum reproductive hormones were detected by Elisa. The levels of luteinizing hormone, luteinizing hormone, follicle-stimulating hormone, follicle-stimulating hormone and inhibin 尾 -Inhibin0 were observed by optical microscope. Real-time PCR method was used to detect the relative expression of inhibin 尾 mRNA in testis of experimental mice, and enzyme linked immunosorbent assay (Elisa) was used to detect the antioxidant index in testis of experimental mice. The contents of Glutathione, GSH, Superoxide dismutase, SODX, malondialdehyde (MDA) and malondialdehyde (MDAs) were observed in Glutathione, glutathione, superoxide dismutase (SOD), malondialdehyde (MDA) and malondialdehyde (MDAs). Results compared with the normal group, the weight of testis was decreased in the weight of testis, especially in the two groups. The level of serum reproductive hormone (FSH) increased significantly (P0.05), while the level of inhibin P decreased significantly (P0.05). Under light microscope, the testis of mice were found to be testicular. Spermatocytes and Sertoli cells were damaged to varying degrees. In group D, the degree of injury was the most obvious, the expression of inhibin 尾 mRNA in testis was obviously decreased, the difference was statistically significant, and the content of SOD in testis showed a decreasing trend (P 0.05). The difference was statistically significant (P 0.05), and the MDA content was on the rise, and the difference was statistically significant (P 0.05). Conclusion the structure and function of testicular Sertoli cells were significantly damaged by W60Co- 緯 rays, and the degree of injury was positively correlated with the dose of irradiation, and the mechanism of injury was related to the damage of antioxidant system of testis induced by radiation. It is related to the oxidative stress damage caused by the imbalance of oxidation-oxidation system in testis.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R818.74

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