發(fā)布時間：2018-04-15 09:31

  本文選題：山菠菜多糖 + 鐵代謝　； 參考：《沈陽體育學院學報》2016年05期

【摘要】：為了探討補充山菠菜多糖對長時間大強度運動大鼠鐵代謝的影響及可能的分子機制,選取30只SD大鼠分為對照組(C組)、運動組(E組)和運動+干預組(EI組),利用跑臺運動構建長時間大強度運動大鼠模型。從跑臺運動第3周起,大鼠用100 mg/kg的山菠菜提取物懸濁液每天灌服2 ml,灌服3周后測定其血清Hepcidin、TF和鐵狀態(tài),實時熒光定量PCR技術檢測肝臟組織中TGF-β1、BMP-2和Smad4基因表達,Western blot法檢測肝臟組織中IL蛋白和JAK/STAT信號通路蛋白表達。結果:與E組相比,補充山菠菜多糖能夠有效降低血清Hepcidin和TF,改善機體鐵缺乏狀態(tài),差異有統(tǒng)計學意義(P0.05),補充山菠菜多糖可有效抑制TGF-β1/BMP/Smad信號通路,也可通過降低肝臟組織中IL-6蛋白表達,而抑制JAK/STAT信號通路蛋白活性,差異有統(tǒng)計學意義(P0.05)。結論:補充山菠菜多糖可通過抑制TGF-β1/BMP/Smad信號通路及IL-6介導的JAK/STAT信號通路而減少Hepcidin表達,改善長時間大強度運動大鼠鐵缺乏狀態(tài)。
[Abstract]:In order to investigate the effect of supplementation of spinach polysaccharide on iron metabolism and its possible molecular mechanism in rats with long time and high intensity exercise,Thirty Sprague-Dawley rats were divided into two groups: control group (C group), exercise group (group E) and exercise intervention group (group I).From the third week of treadmill exercise, rats were given 2 ml of spinach extract suspension (100 mg/kg) daily. After 3 weeks of administration, the serum levels of hepcidinine TF and iron were measured.The expression of BMP-2 and Smad4 in liver tissue was detected by real-time fluorescence quantitative PCR. The expression of IL protein and JAK/STAT signal pathway protein in liver tissue was detected by Western blot.Results: compared with group E, supplementation of mountain spinach polysaccharides could effectively reduce serum Hepcidin and TFFs and improve iron deficiency status. The difference was statistically significant (P 0.05). Supplementation of mountain spinach polysaccharides could effectively inhibit TGF- 尾 1/BMP/Smad signaling pathway.The activity of JAK/STAT signaling pathway protein could be inhibited by decreasing the expression of IL-6 protein in liver tissue, and the difference was statistically significant (P 0.05).Conclusion: supplementation of spinach polysaccharides can reduce the expression of Hepcidin by inhibiting TGF- 尾 1/BMP/Smad signaling pathway and JAK/STAT signaling pathway mediated by IL-6, and ameliorate iron deficiency in rats with long time and high intensity exercise.
【作者單位】： 吉林大學體育學院;
【基金】：吉林省發(fā)改委產業(yè)技術研究與開發(fā)項目(2016C055-3)
【分類號】：R87

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