本文選題：有氧運(yùn)動(dòng)　切入點(diǎn)：BKca通道　出處：《北京體育大學(xué)》2012年碩士論文

【摘要】：研究目的：觀察有氧運(yùn)動(dòng)對(duì)大鼠腦動(dòng)脈平滑肌細(xì)胞BKCa通道生物物理特性的影響。 研究方法：8周齡雄性Wistar大鼠30只,隨機(jī)分為三組,安靜對(duì)照組(SED),高頻運(yùn)動(dòng)組(EX-5d／w)和低頻運(yùn)動(dòng)組(EX-3d／w)各10只。運(yùn)動(dòng)組進(jìn)行12周跑臺(tái)運(yùn)動(dòng)(0°slope,20m、min,50-55％VO2max,60min/day),其中EX-3d／w組每周運(yùn)動(dòng)3次,EX-5d／w組每周運(yùn)動(dòng)5次。12周后,將大鼠麻醉,斷頭處死,迅速打開顱腔取腦。小心取出基底動(dòng)脈及Willis環(huán),剝除周圍結(jié)締組織,經(jīng)酶法消化后得到單個(gè)腦動(dòng)脈平滑肌細(xì)胞。膜片鉗實(shí)驗(yàn)采用單通道內(nèi)面向外模式記錄,觀察各組大鼠腦動(dòng)脈BKCa通道的生物物理特性。 研究結(jié)果：(1)BKCa通道鑒定：本實(shí)驗(yàn)單通道電導(dǎo)為210.5±10,3pS(R2=0.996,n=8)；記錄電流反轉(zhuǎn)電位接近OmV,無整流現(xiàn)象,均符合BKCa單通道特征。(2)有氧運(yùn)動(dòng)使BKCa通道活性提高：膜電位+40mV,[Ca2+]i為1μM時(shí),SED、EX一3d/w和EX-5d／w各組BKCa通道平均開放概率分別為0.28±0.015,0.47±0.02,0.78±0.067。(3)有氧運(yùn)動(dòng)使BKCa通道電壓依賴性增強(qiáng)：[Ca2+]i為1μM時(shí),SED. EX-3d/w. EX-5d/w三組(Normal ized Npo)-V曲線依次左移,三組V1／2分別為44.52mV,37.08mV,26.55mV.(4)有氧運(yùn)動(dòng)使BKCa通道鈣敏感性增強(qiáng)：膜電位+40mV下,SED、EX-3d/w. EX-5d/w三組(Normalized NPo)-Log[Ca2+](?)曲線依次左移,Kd值分別為2.801±0.086μM,1.39±0.079μM,0.35±0.087μM。(5)運(yùn)動(dòng)組大鼠BKCa通道平均開放時(shí)間(To)增大;平均關(guān)閉時(shí)間(Tc)減小,提示有氧運(yùn)動(dòng)使通道更易開放且更難關(guān)閉。 結(jié)論：長(zhǎng)期規(guī)律性有氧運(yùn)動(dòng)能改變大鼠腦動(dòng)脈平滑肌細(xì)胞BKCa通道的門控特性,使其活性增強(qiáng),這可能是運(yùn)動(dòng)改善腦血管舒縮功能,增強(qiáng)腦動(dòng)脈供血.的重要機(jī)制之一；有氧運(yùn)動(dòng)改善BKCa通道活性的效果可能呈運(yùn)動(dòng)量依賴性。
[Abstract]:Aim: to observe the effects of aerobic exercise on the biophysical properties of BKCa channels in rat cerebral artery smooth muscle cells.Methods Thirty eight week-old male Wistar rats were randomly divided into three groups: the quiet control group, the high frequency exercise group (EX-5d / w) and the low-frequency exercise group (EX-3d / w).The rats in the exercise group were treated with 0 擄slopetrope (20 擄slopetrol) for 12 weeks. The rats in the EX-3d/w group were treated with EX-5 d / w 3 times a week for 5 times a week. The rats were anesthetized and killed by severed, and the brain was quickly opened to the cranial cavity for brain extraction. The rats in the EX-3d/w group were treated with EX-5d / w exercise three times a week. The rats were anesthetized, killed and quickly opened to the skull for brain extraction.The basilar artery and Willis ring were carefully removed and the connective tissue around them was stripped. The smooth muscle cells of single cerebral artery were obtained by enzymatic digestion.Single channel inward and outward mode was used to observe the biophysical characteristics of BKCa channel in the cerebral artery of rats in each group.The results showed that the single channel conductance of this experiment was 210.5 鹵10 ~ 3pSN ~ (2) 0.996N ~ (-1) N ~ (8), the current reversal potential was close to OmV, and there was no rectifying phenomenon.EX-3 d / w.The normal ized Npo)-V curve of the three groups of EX-5d/w shifted to the left in turn. The V1 / 2 of the three groups was 44.52 MV, 37.08 MV, 26.55 MV. 4) aerobic exercise enhanced the calcium sensitivity of the BKCa channel: SEDX EX-3 d / w at the membrane potential 40mV.EX-5d/w NPo)-Log [Ca2]The mean opening time of BKCa channel increased and the mean closing time decreased in the exercise group, indicating that aerobic exercise could make the channel more open and difficult to close. The curve shifted to the left and the KD value was 2.801 鹵0.086 渭 MU 1.39 鹵0.079 渭 MU 0.35 鹵0.087 渭 M.M.) in the exercise group, the mean opening time of the BKCa channel increased and the mean closing time decreased, indicating that aerobic exercise made the channel easier to open and harder to close.Conclusion: Long-term regular aerobic exercise can change the gated characteristics of BKCa channel in rat cerebral artery smooth muscle cells and increase its activity, which may be due to the improvement of cerebral vasomotor function and the enhancement of cerebral artery blood supply by exercise.The effect of aerobic exercise on improving BKCa channel activity may be in a dose-dependent manner.
【學(xué)位授予單位】：北京體育大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：G804.2

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