本文選題：力竭運動　切入點：骨骼肌超微結(jié)構(gòu)　出處：《揚州大學(xué)》2012年碩士論文

【摘要】：目的：研究一次力竭性離心運動及恢復(fù)期間大鼠骨骼肌超微結(jié)構(gòu)以及機械生長因子MGF的變化情況。 方法：實驗一：本研究以24只7周左右的健康雄性SD大鼠為研究對象,并隨機分為安靜對照組(Control)；力竭運動即刻組(E0)；力竭運動后24h組(E24)；力竭運動后48h組(E48)共四組,每組各6只。各力竭運動組進(jìn)行一次性力竭(下坡跑)運動,在力竭運動后的不同時間點宰殺取樣。所有大鼠用烏拉坦腹腔麻醉、宰殺后迅速取股外側(cè)肌、股直肌各一分為二。其中一部分用2.5%戊二醛固定,用于制作電鏡超薄切片,在透射電子顯微鏡下觀察骨骼肌超微結(jié)構(gòu)的變化情況,另一部分保存于液氮中冷凍,用于檢測大鼠股外側(cè)肌中機械生長因子MGF的情況。 實驗二：選用4只7周齡成年大鼠和1只新生大鼠,其中成年鼠分組為：安靜對照組1只,鈍挫傷即刻組1只,鈍挫傷24h組1只,鈍挫傷48h組1只。采用急性鈍挫傷模型,分別于鈍挫傷后不同時間點宰殺,迅速取股外側(cè)肌并進(jìn)行即刻培養(yǎng)。采用倒置相差顯微鏡隨時跟拍每天衛(wèi)星細(xì)胞培養(yǎng)情況。 結(jié)果： (1)成年大鼠經(jīng)過一次性力竭離心運動之后,電鏡下觀察其股直肌的超微結(jié)構(gòu)發(fā)現(xiàn),安靜對照組大鼠骨骼肌的超微結(jié)構(gòu)正常。在一次性力竭運動后即刻E0組出現(xiàn)了炎性細(xì)胞,間質(zhì)血管的改變,而力竭運動24小時后即E24組可見骨骼肌細(xì)胞、線粒體和內(nèi)質(zhì)網(wǎng)均出現(xiàn)扭曲、腫脹等形態(tài)改變,Z線、M線明顯異常,嵴紊亂等組織學(xué)變化情況。 (2)本研究采用酶聯(lián)免疫吸附法即ELISA檢測大鼠血清和骨骼肌中MGF的結(jié)果顯示,一方面,從血清中MGF的變化情況來看,力竭運動組E0、E24、E48與安靜對照組相比呈升高趨勢,具有非常顯著性差異(P0.01)。另一方面,從骨骼肌中的MGF的變化來看,E0、E24、E48與安靜對照組相比,都有升高趨勢,其中E24組與安靜對照組相比具有非常顯著性的差異(P0.01)。 (3)在安靜對照組中發(fā)現(xiàn)骨骼肌衛(wèi)星細(xì)胞最初呈圓形,多懸浮于培養(yǎng)液中,1天后細(xì)胞逐漸貼壁生長,細(xì)胞較小,分布均勻,多呈圓形,此后細(xì)胞體積逐漸長大,開始分裂,鏡下可以看見3-5個或多個細(xì)胞成串排列。新生鼠的肌衛(wèi)星細(xì)胞的培養(yǎng)中,與成年大鼠比較,肌衛(wèi)星細(xì)胞的數(shù)目較多。肌衛(wèi)星細(xì)胞貼壁培養(yǎng)48小時后開始增殖,肌肉衛(wèi)星細(xì)胞在第5-8天增殖較快,之后增殖減慢。 結(jié)論： (1)一次性力竭運動后大鼠骨骼肌超微結(jié)構(gòu)出現(xiàn)顯著性的組織學(xué)變化,例如肌原纖維排列混亂,結(jié)構(gòu)破壞,M線、Z線不夠清晰,甚至相鄰間斷裂,H帶、工帶界限模糊,細(xì)胞內(nèi)質(zhì)網(wǎng)線粒體均發(fā)生形變,細(xì)胞間質(zhì)水腫明顯等現(xiàn)象。 (2)一次性力竭運動及恢復(fù)期間,與安靜對照組相比,大鼠血清和骨骼肌中機械生長因子(MGF)在力竭后即刻,24小時和48小時呈現(xiàn)增高的趨勢,且在24小時后達(dá)到峰值,提示MGF可能加快骨骼肌組織和血管再生修復(fù)。 (3)大鼠骨骼肌衛(wèi)星細(xì)胞體外培養(yǎng)需要較高的條件要求,鈍挫傷后骨骼肌衛(wèi)星細(xì)胞的增殖能力比安靜對照組的增殖能力要強。并且新生大鼠衛(wèi)星細(xì)胞的增殖較強。有關(guān)大鼠骨骼肌衛(wèi)星細(xì)胞培養(yǎng)的分化條件還有待進(jìn)一步研究。
[Abstract]:Objective : To study the ultrastructure of skeletal muscle and the changes of mechanical growth factor ( MGF ) in rat skeletal muscle during exercise and recovery .

METHODS : Experiment 1 : Twenty - four healthy male SD rats were randomly divided into two groups : control group ;
exhaustive exercise immediate group ( E0 ) ;
24h after exhaustive exercise ( E24 ) ;
Forty - four groups ( E48 ) were divided into four groups in 48 groups ( E48 ) after exhaustive exercise . All the rats were slaughtered at different time points after exhaustive exercise . All the rats were anesthetized with uranotan , and the rectus muscle and rectus muscle were divided into two groups . One part was fixed with 2.5 % glutaraldehyde . The changes of ultrastructure of skeletal muscle were observed under transmission electron microscope .

Experiment 2 : Four 7 - week - old adult rats and 1 new - born rats were selected . The adult rats were randomly divided into two groups : one in the quiet control group , one in the immediate group in the blunt contusion group , one in the blunt - contusion group and one in the blunt - contusion 48 - hour group .

Results :

( 1 ) The ultrastructure of the rectus muscle was observed under the electron microscope after one - time exhaustive exercise . The changes of inflammatory cells and interstitial vessels were observed in the E24 group immediately after one - time exhaustive exercise .

( 2 ) In this study , the results of MGF in serum and skeletal muscle of rats were determined by enzyme linked immunosorbent assay ( ELISA ) . On the one hand , the levels of E0 , E24 , E48 in exhaustive exercise group were higher than those in the quiet control group ( P0.01 ) .

( 3 ) In the quiet control group , it was found that the skeletal muscle satellite cells were initially round and multi - suspended in the culture medium . After 1 day , the cells grew gradually , the cells were smaller , the distribution was uniform , and the number of cells was more round . After that , the number of muscle satellite cells grew up , and the number of muscle satellite cells began to split . After 48 hours , the muscle satellite cells began to proliferate and the muscle satellite cells grew faster in the 5th - 8 days , and then the proliferation was slowed down .

Conclusion :

( 1 ) There were significant histological changes in the ultrastructure of skeletal muscle of rats after one - time exhaustive exercise , such as confusion of myofibril arrangement , structural damage , M - ray , lack of clarity in Z - line , even adjacent fracture , H - band , blurring of the boundary of the working band , deformation of mitochondria in the endoplasmic reticulum of the cell , and obvious cell - interstitial edema .

( 2 ) During the exercise and recovery of one - time exhaustive exercise , the mechanical growth factor ( MGF ) in serum and skeletal muscle of rats showed an increased tendency immediately after exhaustion , and peaked at 24 hours and 48 hours , and peaked at 24 hours , suggesting that MGF could accelerate the repair of skeletal muscle tissue and vascular regeneration .

( 3 ) In vitro culture of rat skeletal muscle satellite cells requires higher conditions , and the proliferation ability of skeletal muscle satellite cells after blunt trauma is stronger than that in the quiet control group .

【學(xué)位授予單位】：揚州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R873;G804.2

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 段昌平 ,盧鼎厚 ,傅湘琦 ,趙天德;針刺和靜力牽張對延遲性酸痛過程中骨骼肌超微結(jié)構(gòu)的影響[J];北京體育學(xué)院學(xué)報;1984年04期

2 蔡賢德;;運動對骨骼肌形態(tài)結(jié)構(gòu)的影響研究[J];才智;2009年29期

3 賀道遠(yuǎn);;機械生長因子在骨骼肌活動中的作用[J];長江大學(xué)學(xué)報(自然科學(xué)版)理工卷;2008年01期

4 羅吉偉,余斌,覃承訶,楊建成,林文_",翁錫源,黃麗英;反復(fù)力竭運動后大鼠骨骼肌線粒體超微結(jié)構(gòu)改變及維生素E的保護(hù)作用[J];第一軍醫(yī)大學(xué)學(xué)報;2003年12期

5 張臻,鐘z，

本文編號：1690868