本文選題：遞增負(fù)荷運(yùn)動(dòng)　切入點(diǎn)：促腎上腺皮質(zhì)激素受體mRNA　出處：《西安體育學(xué)院學(xué)報(bào)》2017年01期

【摘要】：目的探討6周遞增跑臺(tái)運(yùn)動(dòng)對(duì)大鼠血清GC和腎上腺ACTH、GC受體的影響。方法 SPF級(jí)雄性SD大鼠128只,隨機(jī)分成16組,分別是完全不訓(xùn)練組(A),共4小組,每小組8只;處死當(dāng)天不訓(xùn)練組(B),共4小組,每小組8只;運(yùn)動(dòng)后即刻處死組(C),共4小組,每小組8只;運(yùn)動(dòng)后3 h處死組(D),共4組,每小組8只。A組在整個(gè)實(shí)驗(yàn)過程中不訓(xùn)練,B、C、D組進(jìn)行6周遞增負(fù)荷跑臺(tái)運(yùn)動(dòng)。分別在第0、2、4、6周周末處死A、B、C、D組各一小組。取腎上腺和血液,用Real-time PCR測(cè)試各小組腎上腺ACTH-R mRNA和GR mRNA的表達(dá),用ELISA法測(cè)試各小組血清中GC的含量。結(jié)果第0、2、4、6周末4組大鼠的腎上腺中ACTH-R mRNA在第0周、第2周末和第6周末的表達(dá)沒有顯著性差異(P㧐0.05),只是在第4周末B組和C組比A組和D組有顯著性升高(P0.05);各組GR mRNA在第0周和第2周末無顯著性差異,第4周末和第6周末的C組和D組比其它兩組有極顯著性升高(P0.001);各周末血清中GC含量都表現(xiàn)為C組和D組比其它兩組有顯著性升高(P0.05)。結(jié)論 6周遞增負(fù)荷運(yùn)動(dòng)過程中,腎上腺ATCH-R mRNA表達(dá)表現(xiàn)出時(shí)相性和飽和性,其表達(dá)并沒有和ACTH陽(yáng)性細(xì)胞的表達(dá)保持一致,二者具有不同步性,可能是ACTH在運(yùn)輸或在正、負(fù)反饋調(diào)節(jié)環(huán)節(jié)受到調(diào)控的結(jié)果。隨著運(yùn)動(dòng)強(qiáng)度的增大,機(jī)體的中GR mRNA表達(dá)的含量降低,其表達(dá)趨勢(shì)和GC分泌趨勢(shì)相反,這應(yīng)該是機(jī)體保持自身穩(wěn)態(tài)的保護(hù)機(jī)制之一。
[Abstract]:Objective to investigate the effect of 6-week incremental treadmill exercise on serum GC and adrenal ACTHN GC receptors in rats. Methods SPF grade male SD rats were randomly divided into 16 groups with 8 rats in each group. On the day of death, 4 groups (8 rats per group) were killed without training, 4 groups (8 rats) were killed immediately after exercise, 4 groups (8 rats per group) were killed immediately after exercise, and 4 groups (4 groups) were killed 3 hours after exercise. During the whole experiment, 8 rats in group A were killed at the end of the 6th week, and the adrenal gland and blood were taken from adrenal gland and blood. The expression of ACTH-R mRNA and gr mRNA in adrenal gland was measured by Real-time PCR, and the content of GC in serum was measured by ELISA method. Results ACTH-R mRNA in adrenal gland of 4 groups was detected at the end of the 6th week. There was no significant difference in expression between the 2nd weekend and the 6th weekend. There was no significant difference in gr mRNA between group B and group C at the end of the 4th week compared with that in group A and group D, but there was no significant difference in gr mRNA between group A and group D at the end of week 0 and week 2, but there was no significant difference between group B and group C at the end of the 4th week. At the end of the 4th and 6th weekend, the serum GC levels in group C and group D were significantly higher than those in group C and group D compared with the other two groups. Conclusion during 6 weeks of incremental load exercise, the serum GC levels in group C and group D were significantly higher than those in group C and group D, respectively. Conclusion during 6 weeks of incremental load exercise, the serum GC levels in group C and D were significantly higher than those in group C and group D, respectively. The expression of ATCH-R mRNA in adrenal gland showed chronotropic and saturation, and its expression was not consistent with the expression of ACTH positive cells. The expression of ACTH was different from that of ACTH positive cells, and it may be that the expression of ACTH was in transit or positive. With the increase of the intensity of exercise, the expression of gr mRNA in the body decreased, which was contrary to the tendency of GC secretion, which should be one of the protective mechanisms to maintain the homeostasis of the body.
【作者單位】： 山東師范大學(xué)體育學(xué)院;華南師范大學(xué)體育科學(xué)學(xué)院;
【基金】：廣東省自然科學(xué)基金項(xiàng)目(9151063101000059)
【分類號(hào)】：R87

【參考文獻(xiàn)】

相關(guān)期刊論文 前7條

1 覃飛;郝選明;;長(zhǎng)期遞增負(fù)荷運(yùn)動(dòng)對(duì)小腸集合淋巴結(jié)結(jié)構(gòu)及淋巴細(xì)胞凋亡的影響[J];體育學(xué)刊;2012年01期

2 王茂葉;郝選明;;6周遞增跑臺(tái)運(yùn)動(dòng)對(duì)大鼠下丘腦室旁核CRH細(xì)胞及腺垂體ACTH細(xì)胞的影響[J];西安體育學(xué)院學(xué)報(bào);2011年02期

3 耿青青;郝選明;;骨髓中IL-7、IL-7R及EBF表達(dá)與運(yùn)動(dòng)性免疫抑制[J];體育學(xué)刊;2011年01期

4 王茂葉;;6周遞增跑臺(tái)運(yùn)動(dòng)對(duì)大鼠血清Th1/Th2平衡和IL-6的影響[J];體育科學(xué);2009年10期

5 蔡斌,陳佩杰;運(yùn)動(dòng)與糖皮質(zhì)激素受體[J];中國(guó)臨床康復(fù);2002年15期

6 陳佩杰;沈步乙;陶心銘;徐仁寶;;運(yùn)動(dòng)時(shí)糖皮質(zhì)激素分泌增多對(duì)大鼠心、腎糖皮質(zhì)激素受體及 Na~+-K~+-ATP 酶的影響和機(jī)制分析[J];體育科學(xué);1993年02期

7 陳佩杰,韋俊文,方明,徐仁寶;老年人長(zhǎng)跑鍛練后白細(xì)胞介素-1的變化及其和糖皮質(zhì)激素受體的可能關(guān)系[J];生理學(xué)報(bào);1992年02期

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 王茂葉;郝選明;;6周遞增跑臺(tái)運(yùn)動(dòng)對(duì)大鼠腎上腺ACTH、GC受體和血清GC的影響[J];西安體育學(xué)院學(xué)報(bào);2017年01期

2 易學(xué);;運(yùn)動(dòng)強(qiáng)度對(duì)免疫反應(yīng)可起到雙向調(diào)控的影響效應(yīng)[J];中國(guó)組織工程研究;2015年46期

3 王雪芹;郝選明;;長(zhǎng)期遞增負(fù)荷運(yùn)動(dòng)對(duì)大鼠脾淋巴細(xì)胞增殖活性、凋亡及凋亡相關(guān)基因表達(dá)的影響[J];中國(guó)體育科技;2013年06期

4 李化光;劉華;顏進(jìn)項(xiàng);王軍;劉肅;楊新明;;生肌象皮膏治療創(chuàng)傷性皮膚缺損初步探討[J];河北北方學(xué)院學(xué)報(bào)(自然科學(xué)版);2013年03期

5 王雪芹;;運(yùn)動(dòng)與免疫細(xì)胞凋亡及其機(jī)制研究現(xiàn)狀[J];中國(guó)運(yùn)動(dòng)醫(yī)學(xué)雜志;2013年06期

6 曹偉;郝選明;;六周遞增負(fù)荷運(yùn)動(dòng)對(duì)大鼠胸腺蛋白組表達(dá)的影響[J];體育科學(xué);2013年06期

7 李東哲;馬維民;;鎖陽(yáng)煎劑對(duì)運(yùn)動(dòng)大鼠肝臟自由基代謝及肝糖原代謝的影響[J];現(xiàn)代預(yù)防醫(yī)學(xué);2013年08期

8 王雪芹;郝選明;;6周遞增負(fù)荷運(yùn)動(dòng)對(duì)大鼠小腸集合淋巴結(jié)細(xì)胞線粒體凋亡途徑的影響[J];體育學(xué)刊;2013年02期

9 耿青青;;遞增負(fù)荷運(yùn)動(dòng)對(duì)大鼠早期B細(xì)胞發(fā)育數(shù)量及凋亡情況的影響[J];山東體育學(xué)院學(xué)報(bào);2013年01期

10 王雪芹;郝選明;;運(yùn)動(dòng)與免疫研究進(jìn)展[J];中國(guó)運(yùn)動(dòng)醫(yī)學(xué)雜志;2012年12期

【二級(jí)參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 王茂葉;;6周遞增跑臺(tái)運(yùn)動(dòng)對(duì)大鼠血清Th1/Th2平衡和IL-6的影響[J];體育科學(xué);2009年10期

2 謝東北;郝選明;;運(yùn)動(dòng)與免疫關(guān)系研究進(jìn)展述評(píng)[J];體育學(xué)刊;2009年05期

3 白雪飛;;黏膜免疫系統(tǒng)相關(guān)免疫細(xì)胞、免疫分子及淋巴細(xì)胞歸巢研究進(jìn)展[J];河南醫(yī)學(xué)研究;2008年03期

4 徐唯;顏軍;;中小負(fù)荷運(yùn)動(dòng)對(duì)急性心理應(yīng)激大鼠血清IFN-γ和IL-4的影響[J];中國(guó)體育科技;2008年05期

5 徐唯;黃彬;;運(yùn)動(dòng)與IFN-γ和IL-4[J];中國(guó)醫(yī)療前沿;2008年17期

6 陳德權(quán);鄧樹勛;彭峰林;;間歇運(yùn)動(dòng)對(duì)大鼠骨骼肌凋亡基因Bax、Bcl-2及相關(guān)因素的影響[J];北京體育大學(xué)學(xué)報(bào);2008年07期

7 劉承宜;袁建琴;付德榮;王海霞;李江華;;以賽帶練的內(nèi)穩(wěn)態(tài)研究[J];體育學(xué)刊;2008年05期

8 王今越;丁樹哲;劉偉;王小虹;;運(yùn)動(dòng)與IL-6的研究進(jìn)展[J];體育科學(xué);2007年06期

9 劉承宜;袁建琴;陳少華;;科學(xué)訓(xùn)練、健康傳播與奧運(yùn)的平民化戰(zhàn)略[J];體育學(xué)刊;2007年03期

10 王純;孫君志;王東輝;蘇全生;;大鼠運(yùn)動(dòng)后不同時(shí)相血漿IL-6變化的比較[J];成都體育學(xué)院學(xué)報(bào);2006年04期

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