本文選題：胰島素樣生長因子1　切入點：胰島素樣生長因子1受體　出處：《蘇州大學》2012年碩士論文

【摘要】：研究目的： 隨著人類邁入老齡化社會，發(fā)生老年相關(guān)性疾病的人數(shù)也在不斷增加，成為一個極具潛在威脅的公共衛(wèi)生問題。骨骼肌是人體最重要的運動器官，在衰老過程中骨骼肌機能的下降是衰老的重要標志之一。骨骼肌衰老的預防和干預是重要的醫(yī)學問題和前沿課題，因而運動延緩骨骼肌衰老的作用及其機制研究具有重要的理論和實際意義。力量訓練對控制體重、預防骨質(zhì)疏松及防治Ⅱ型糖尿病有積極作用,但在老年人群的健身實踐中，其作用一直沒有得到重視和充分發(fā)揮。研究表明，衰老過程中骨骼肌質(zhì)量、力量和活力的下降可能與肌肉再生能力受損（衛(wèi)星細胞和蛋白質(zhì)更新缺陷）、氧化應激水平上升、運動神經(jīng)元丟失、內(nèi)分泌紊亂、免疫系統(tǒng)功能衰退、慢性炎癥狀態(tài)以及骨骼肌細胞凋亡等多種因素有關(guān)，但其發(fā)生機制尚缺乏完備的理論。本研究采用老齡大鼠（18月齡）為實驗對象，探討有氧運動（游泳）和抗阻力訓練（爬梯運動）對老齡大鼠骨骼肌形態(tài)結(jié)構(gòu)和IGF-1/IGF-1R信號通路及線粒體介導的細胞凋亡信號通路的影響，為闡明有氧運動和力量訓練干預衰老性肌萎縮的機制、臨床預防和治療提供理論和實驗依據(jù)。 研究方法： 老齡（18月齡）健康雄性Sprague-Dawley大鼠36只，隨機分為安靜對照組（C）、抗阻力訓練組(LC)、負重游泳組(SW1)、無負重游泳組(SW2)，每組各9只。游泳組大鼠每周訓練5天，每天訓練30min,共9周；負重游泳組大鼠負重為體重的1%；抗阻力訓練組大鼠每周訓練3天，共訓練9周，起始負荷為大鼠體重的10%，每周遞增體重的10%。通過光鏡觀察衰老大鼠腓腸肌肌纖維直徑的變化、Elisa檢測骨骼肌IGF-1、IGF-1R及免疫印跡分析檢測骨骼肌線粒體與胞漿內(nèi)細胞素色c，采用Graphpad5.0軟件對結(jié)果進行單因素方差分析。 結(jié)果： （1）與對照組相比，，抗阻力訓練組肌纖維直徑顯著增大（P0.01）負重游泳組、無負重游泳組腓腸肌直徑無顯著變化（P0.05）；抗阻力訓練組大鼠肌纖維直徑顯著大于無負重游泳組、負重游泳組（P0.01）。 （2）與對照組相比，抗阻力訓練組大鼠腓腸肌IGF-1與IGF-1R表達上調(diào)。（P0.01）；與對照組相比，無負重游泳組大鼠腓腸肌IGF-1與IGF-1R表達下調(diào)。（P0.01）；負重游泳組與對照組相比，IGF-1表達無明顯變化（P0.05），IGF-1R表達下調(diào)（P0.01）；抗阻力訓練組大鼠腓腸肌IGF-1與IGF-1R表達顯著高于無負重游泳組、負重游泳組（P0.01，P0.01）。 （3）與對照組相比，抗阻力訓練組大鼠腓腸肌線粒體細胞色素c含量顯著增加（P0.01），胞漿內(nèi)細胞色素c水平降低（P0.01），負重游泳組腓腸肌線粒體細胞色素c含量無明顯變化（P0.05），無負重游泳組線粒體細胞色素c含量增加（P0.05）,無負重游泳組和負重游泳組大鼠腓腸肌胞漿內(nèi)細胞色素c有減少的趨勢，無顯著性差異（P0.05）；負重游泳組、無負重游泳組腓腸肌線粒體細胞色素c含量均顯著低于抗阻力訓練組（P0.01，P0.05）；負重游泳組腓腸肌胞漿內(nèi)細胞色素c水平顯著高于抗阻力訓練組（P0.01），無負重游泳組與抗阻力訓練組相比，胞漿內(nèi)細胞色素c含量無明顯差異（P0.05）。 結(jié)論： （1）在提高老齡大鼠腓腸肌的橫截面積、誘發(fā)肌肉肥大方面，抗阻力訓練優(yōu)于有氧運動，表明抗阻力訓練是干預衰老性肌萎縮發(fā)生的有效手段，對于預防和延緩衰老性肌萎縮的發(fā)生有重要的意義，在老年人運動中應積極推廣。 （2）抗阻力訓練能顯著提高IGF-1和IGF-1R水平，可能是抗阻力訓練預防衰老性肌萎縮的機制。 （3）抗阻力訓練能更有效地抑制線粒體釋放細胞色素c進入胞漿，在一定程度上減輕了老齡大鼠腓腸肌凋亡程度，可能是在預防衰老性肌萎縮方面抗阻力訓練優(yōu)于有氧運動的機制。
[Abstract]:The purpose of the study is:
With the rapid development of the aging society, the number of occurrence of age-related diseases is also increasing, become a public health problem a great potential threat. Skeletal muscle is the most important organ of the human body movement in the aging process, decrease the skeletal muscle function is one of the important signs of aging. The prevention and intervention of skeletal muscle aging is medical problems and important subject, thus retard the effect and mechanism of skeletal muscle aging has important theoretical and practical significance. The strength training for weight control, prevention of osteoporosis and the prevention and treatment of type II diabetes has a positive effect, but in practice the elderly fitness, its role has not been taken seriously and fully. Research shows that the skeletal muscle aging process quality, decrease the strength and vitality of the possibility and impaired muscle regeneration (satellite cells and proteins, oxidative stress update defects) Shock levels rise, loss of motor neurons, endocrine disorders, immune function decline, various factors of chronic inflammatory state and skeletal muscle cell apoptosis, but its mechanism is still lack of complete theory. This study used in aged rats (18 month old) as the object of study, aerobic exercise (swimming) and resistance training (ladder climbing exercise) effect on the signal pathway of apoptosis in aging rat skeletal muscle morphology and IGF-1/IGF-1R signaling pathway and mitochondrial mediated, to clarify the mechanism of aerobic exercise and strength training intervention of age-related muscle atrophy, and provide theoretical and experimental basis for clinical prevention and treatment.
Research methods:
(aged 18 month old) in 36 healthy male Sprague-Dawley rats, were randomly divided into control group (C), the resistance training group (LC), swimming group (SW1), swimming group (SW2), 9 rats in each group. Rats swimming training 5 days a week, every day of training 30min, Co 9 weeks; swimming rats weight weight 1% rats per week training; resistance training 3 days, training 9 weeks. The initial load for the weight of rats 10%, weight 10%. weekly increments changes of gastrocnemius muscle of aged rats diameter was observed by light microscopy, Elisa detection of IGF-1 in skeletal muscle analysis, detection of skeletal muscle mitochondrial and cytosolic cytochrome c IGF-1R and Western blotting, single factor analysis of variance on the results by Graphpad5.0 software.
Result錛

本文編號：1667003