本文選題：肺癌　切入點：VEGF　出處：《第二軍醫(yī)大學(xué)》2017年碩士論文

【摘要】：目的:比較不同對比劑在離體腫瘤血管成像中的作用。比較不同VEGF表達(dá)水平兩種細(xì)胞間的血管定量參數(shù)的差異。貝伐單抗抗血管生成治療后腫瘤血管變化趨勢。方法:構(gòu)建A549細(xì)胞移植瘤模型,并使用碘海醇、硫酸鋇懸混液、Micro-fil制備不同對比劑的瘤血管灌注模型;構(gòu)建A549細(xì)胞和Calu-6細(xì)胞移植瘤模型,采用western-blot法檢測兩種細(xì)胞VEGF表達(dá)量,采用顯微CT監(jiān)測兩細(xì)胞移植瘤模型不同時間點瘤血管定量參數(shù);構(gòu)建A549細(xì)胞移植瘤模型,并采用貝伐單抗干預(yù)和生理鹽水對照,采用顯微CT評價兩組移植瘤模型血管參數(shù)的變化情況。結(jié)果:Micro-fil在離體腫瘤樣本中其對比效果要優(yōu)于硫酸鋇懸混液和碘海醇。高VEGF表達(dá)的Calu-6細(xì)胞模型其腫瘤體積、血管體積、血管節(jié)點總數(shù)、血管節(jié)點密度均高于低VEGF表達(dá)的A549細(xì)胞模型,但是在反映血管三維復(fù)雜程度的分形維度和血管表面積與體積比兩參數(shù)方面,兩者沒有統(tǒng)計學(xué)差異;Calu-6細(xì)胞模型的體積、血管體積、血管節(jié)點總數(shù)等方面隨時間推移而增多,但是在血管體積分?jǐn)?shù)、血管節(jié)點密度等方面隨時間推移而減少;A549細(xì)胞模型的血管體積分?jǐn)?shù)、血管節(jié)點密度在前期隨時間推移而增多,在第19天出現(xiàn)明顯減少。貝伐單抗拮抗的A549細(xì)胞模型,在腫瘤體積、血管體積、血管節(jié)點總數(shù)、血管節(jié)點密度等方面與對照組有明顯差異,但與對照組表現(xiàn)的血管體積相對不足不同,抗VEGF治療組其血管體積分?jǐn)?shù)、血管節(jié)點密度在緩慢上升,雖然結(jié)果沒有統(tǒng)計學(xué)意義。結(jié)論:Micro-fil適合作為離體血管標(biāo)本的對比劑。同時不同VEGF的腫瘤在腫瘤血管定量參數(shù)方面的差異具有顯著統(tǒng)計學(xué)意義,VEGF在腫瘤血管生成中具有重要意義�？筕EGF治療后,腫瘤血管相關(guān)定量參數(shù)明顯減少,但是其血管定量參數(shù)的改變,提示在腫瘤內(nèi)部仍有其他血管生成因子的作用,可能與單純應(yīng)用抗血管生成治療出現(xiàn)耐藥相關(guān)。
[Abstract]:Aim: to compare the effects of different contrast agents on tumor angiography in vitro, and to compare the difference of vascular quantitative parameters between two kinds of cells at different VEGF expression levels. Methods: A549 cell transplantation tumor model was established. The tumor vascular perfusion models with different contrast agents were prepared with iodohexol and barium sulfate suspension solution micro-fil, and the tumor models of A549 cell and Calu-6 cell were constructed, and the expression of VEGF in two kinds of cells was detected by western-blot method. MicroCT was used to monitor the quantitative parameters of tumor at different time points in two-cell transplanted tumor model, and the A549 cell transplantation tumor model was constructed, and bevacizumab intervention and physiological saline control were used to construct A549 cell transplantation tumor model. MicroCT was used to evaluate the changes of vascular parameters in two groups of transplanted tumor models. Results the contrast effect of the two groups was better than that of barium sulfate suspension and iodiol. The tumor volume and vascular volume of the Calu-6 cell model with high VEGF expression were better than those of the isolated tumor samples. The total number of vascular nodes and the density of vascular nodes were higher than those of A549 cell model with low VEGF expression. There was no statistical difference between the two groups in the volume, vascular volume, and the total number of vascular nodes in the Calu-6 cell model over time, but the volume fraction of the blood vessel. The density of vascular node decreased with time, the density of vascular node increased in the early stage and decreased obviously on the 19th day. The A549 cell model was antagonized by bevacizumab. There were significant differences in tumor volume, vascular volume, total number of vascular nodes and density of vascular nodes between the control group and the control group, but the vascular volume fraction of the anti VEGF treatment group was different from that of the control group. The density of vascular nodes is slowly rising. Although the results are not statistically significant. Conclusion: Micro-fil is suitable for comparison of vascular specimens in vitro. Meanwhile, there are significant differences in tumor vascular quantitative parameters between different VEGF and VEGF in tumor angiogenesis. After anti VEGF treatment, The quantitative parameters of tumor angiogenesis were decreased obviously, but the changes of vascular quantitative parameters indicated that there were still other angiogenic factors in the tumor, which might be related to the drug resistance of antiangiogenic therapy alone.
【學(xué)位授予單位】：第二軍醫(yī)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R734.2;R-332;R730.44

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本文編號：1665991