本文選題：~1H-NMR　切入點(diǎn)：彌漫性軸索損傷　出處：《重慶醫(yī)科大學(xué)》2013年碩士論文

【摘要】：目的探討基于1H-NMR的檢測(cè)技術(shù)應(yīng)用于SD大鼠DAI血漿代謝組學(xué)研究的可行性,并尋找篩選早期診斷DAI的可能的特異性生物標(biāo)志物。 方法采用改良的Marmarou A方法建立SD大鼠DAI模型,將所有實(shí)驗(yàn)動(dòng)物隨機(jī)分為5組(空白對(duì)照組(n=6)、手術(shù)1天對(duì)照組(n=6)、打擊致傷1天組(n=8)、手術(shù)3天對(duì)照組(n=6)、打擊致傷3天組(n=8)),收集各組SD大鼠右心室血液,制備成血漿后通過1H-NMR檢測(cè),采用PCA、PLS-DA和OPLS-DA進(jìn)行數(shù)據(jù)處理分析。 結(jié)果與對(duì)照組相比,DAI組SD大鼠血漿中1H-NMR有明顯差異,各組血漿樣本間有32種分子代謝物有顯著差異,DAI1天時(shí)Glu明顯升高,3天時(shí)Lac顯著升高,致傷到傷后3天脂質(zhì)成分(VLDL和LDL)一直顯著降低。 結(jié)論1H-NMR技術(shù)是研究DAI代謝組學(xué)的有效技術(shù)手段。分析鑒定出的32種代謝物及其變化規(guī)律或可為DAI早期診斷特異性生物標(biāo)志物的篩選提供可靠的實(shí)驗(yàn)依據(jù)。
[Abstract]:Objective to explore the feasibility of using 1H-NMR based detection technique in the study of plasma metabolism in SD rats with DAI, and to find out the possible specific biomarkers for early diagnosis of DAI. Methods the DAI model of SD rats was established by modified Marmarou A method. All experimental animals were randomly divided into five groups: control group (control group), control group (control group), control group (control group), control group (control group), control group (control group), control group (control group), control group (control group), control group (control group), control group (control group), control group (n = 6) and control group (n = 8), respectively. Blood samples from right ventricle of SD rats in each group were collected, and plasma was prepared for detection by 1H-NMR. Data processing and analysis were carried out by PCAA PLS-DA and OPLS-DA. Results compared with the control group, there were significant differences in plasma 1H-NMR in SD rats, and there were significant differences in 32 kinds of molecular metabolites among plasma samples of each group. At day 1, Glu was significantly increased and Lac was significantly increased at day 3. VLDL and LDL decreased significantly 3 days after injury. Conclusion the 1H-NMR technique is an effective technique for the study of DAI metabolomics. The analysis and identification of 32 metabolites and their changes may provide a reliable experimental basis for the screening of specific biomarkers for early diagnosis of DAI.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：D919.4

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 何朝暉;孫曉川;李峰;江涌;鄭履平;;軸索損傷后熱休克蛋白70的變化對(duì)Tau蛋白表達(dá)的影響[J];第三軍醫(yī)大學(xué)學(xué)報(bào);2008年09期

2 何光龍;賈冬梅;周亦武;孫來晶;鄧偉年;戴甲培;劉良;徐小虎;;大鼠腦挫傷后腦組織β-APP表達(dá)的研究[J];法律與醫(yī)學(xué)雜志;2006年02期

3 李上勛;劉丹;何光龍;王昊;段yN杰;馬祥濤;周亦武;;彌漫性軸索損傷的研究進(jìn)展[J];中國(guó)法醫(yī)學(xué)雜志;2010年05期

4 余暉;劉靜;沈桂權(quán);魏渝清;王學(xué)建;;聯(lián)合DWI和常規(guī)MRI對(duì)非出血性彌漫性軸索損傷的診斷價(jià)值[J];臨床放射學(xué)雜志;2007年07期

5 王晗;;高血糖加重腦缺血損傷機(jī)制的研究現(xiàn)狀[J];現(xiàn)代生物醫(yī)學(xué)進(jìn)展;2007年09期

6 楊子;賴關(guān)朝;王國(guó)彬;睢罡;李宏玲;黃建勛;唐小江;;SD大鼠腦脊液采集方法改進(jìn)[J];中國(guó)職業(yè)醫(yī)學(xué);2011年02期

7 曹遠(yuǎn)東,章龍珍,王梅申,唐天友,蘇衛(wèi)紅;兩種采集SD大鼠腦脊液方法的比較[J];徐州醫(yī)學(xué)院學(xué)報(bào);2005年04期

8 魏風(fēng);陳儉;;神經(jīng)元特異性烯醇化酶在顱腦損傷中的研究進(jìn)展[J];醫(yī)學(xué)綜述;2012年01期

9 段志新;馬延斌;;彌漫性軸索損傷的影像學(xué)研究進(jìn)展[J];中國(guó)臨床神經(jīng)外科雜志;2008年07期

10 雷益,邱德正,朱嘉英,陳志剛,李頂夫,黨勇,馮偉,孟慶國(guó),李濤;腦彌漫性軸索損傷的MRI表現(xiàn)[J];中華創(chuàng)傷雜志;2004年04期

，

本文編號(hào)：1658820