本文選題：EIMD　切入點(diǎn)：MG53　出處：《首都體育學(xué)院》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：研究目的:常用于監(jiān)控運(yùn)動員運(yùn)動性骨骼肌損傷程度的血液生化指標(biāo)有血清肌酸激酶(CK)、肌紅蛋白(Mb)、血尿素(BUN)等。最近研究發(fā)現(xiàn)一種存在于骨骼肌和心肌中的特異性修復(fù)蛋白MG53(Mitsugumin 53),MG53可以與其它幾種肌肉特異性膜修復(fù)蛋白在功能上協(xié)同作用完成肌細(xì)胞膜損傷后的快速修復(fù)。在此研究基礎(chǔ)上,本文旨在探討運(yùn)動員在不同的有氧運(yùn)動方式和運(yùn)動時間下,骨骼肌損傷后血清中MG53與血清肌酸激酶、肌紅蛋白、血尿素的相關(guān)性。為運(yùn)動性骨骼肌損傷的科學(xué)診斷提供理論支持。研究方法:選取16名男性運(yùn)動員(平均年齡23.13±2.92歲,訓(xùn)練年限5.13±2.92年,國家二級運(yùn)動員)在空腹安靜狀態(tài)下采集靜脈血。隔天在實(shí)驗室進(jìn)行功率車耐力騎行90min運(yùn)動,起始功率為50W,保持60rpm的轉(zhuǎn)速下每分鐘遞增20W直至通氣無氧閾出現(xiàn)對應(yīng)的負(fù)荷量即為功率車負(fù)荷。運(yùn)動前,空腹安靜狀態(tài)下采集靜脈血;運(yùn)動后0h、1h、4h、24h分別采集一次靜脈血。間隔2周進(jìn)行功率車高強(qiáng)度間歇運(yùn)動1min60-90%無氧閾強(qiáng)度、間歇4min20-40%無氧閾強(qiáng)度蹬車共10組。采血方案同上。兩次運(yùn)動測試結(jié)束后間隔一周再次采集靜脈血測定血清中CK、Mb、BUN、MG53蛋白含量。所有測得數(shù)據(jù)結(jié)果采用SPSS 22.0統(tǒng)計軟件進(jìn)行分析。研究結(jié)果:不同時間點(diǎn)與運(yùn)動方式:(1)功率車耐力運(yùn)動中,不同時間點(diǎn)間的血清CK不具有顯著性差異(P0.05)。運(yùn)動后1h、4h和24h的Mb分別較安靜值與運(yùn)動后即刻顯著升高(p0.01)。運(yùn)動后即刻BUN較安靜值有所升高(p0.05),運(yùn)動后1h和4h的BUN較安靜值顯著升高(p0.01)。運(yùn)動后24h的BUN較運(yùn)動后1h和4h的BUN顯著降低(p0.01)。各時間點(diǎn)MG53值無顯著差異(P0.05)。(2)高強(qiáng)度間歇運(yùn)動中,不同時間點(diǎn)間的血清CK不具有顯著性差異(P0.05)。運(yùn)動后1h和24h的Mb較運(yùn)動后即刻顯著升高(p0.01)。不同時間點(diǎn)之間BUN不具有顯著性差異(P0.05)。各時間點(diǎn)MG53值無顯著差異(P0.05)。同一時間點(diǎn)不同運(yùn)動方式:(1)運(yùn)動后4小時,功率車耐力運(yùn)動Mb顯著高于高強(qiáng)度間歇運(yùn)動(p0.05)。(2)運(yùn)動后即刻、1小時、4小時功率車耐力運(yùn)動BUN顯著高于高強(qiáng)度間歇運(yùn)動(p0.05)。MG53與各指標(biāo)之間的相關(guān)性:(1)功率車耐力運(yùn)動中,運(yùn)動后4小時Mb與CK呈正相關(guān)(r=0.755 p0.01),運(yùn)動后24小時Mb與CK呈正相關(guān)(r=0.711 p0.01),MG53在運(yùn)動不同時間點(diǎn)與CK、Mb、BUN均沒有相關(guān)性。(2)高強(qiáng)度間歇運(yùn)動中,各個時間點(diǎn)CK與Mb呈不同程度的相關(guān)性,MG53在運(yùn)動不同時間點(diǎn)與CK、Mb、BUN均沒有相關(guān)性。研究結(jié)論:(1)功率車耐力運(yùn)動與高強(qiáng)度間歇運(yùn)動后,Mb、BUN有顯著性改變。(2)功率車耐力運(yùn)動后即刻、運(yùn)動后4小時血清Mb、BUN變化顯著。(3)人源MG53在兩種有氧運(yùn)動方式運(yùn)動后即刻相比運(yùn)動前含量增加。(4)人源MG53與不同方式有氧運(yùn)動前、運(yùn)動后即刻、運(yùn)動后1小時、4小時、24小時的血清CK、Mb、BUN之間沒有相關(guān)性。(5)有氧運(yùn)動后血清人源MG53與血清CK、Mb變化周期不同。
[Abstract]:Objective: the blood biochemical indexes commonly used to monitor the degree of sports skeletal muscle injury in athletes include serum creatine kinase (CK), myoglobin (Mb), blood urea bun (BUNA) and so on. Recently, a specific type of blood biochemical index was found in skeletal muscle and myocardium. The sex repair protein (MG53(Mitsugumin 53) MG53 can function in synergy with other muscle-specific membrane repair proteins (MG53) to complete the rapid repair of muscle cell membrane damage. The purpose of this study was to investigate the levels of MG53 and creatine kinase, myoglobin in the serum of athletes after skeletal muscle injury under different aerobic exercise patterns and exercise time. Correlation of blood urea. To provide theoretical support for scientific diagnosis of sports skeletal muscle injury. Methods: 16 male athletes (mean age 23.13 鹵2.92 years, training time 5.13 鹵2.92 years) were selected. State level II athletes) collect venous blood in an empty stomach and quiet state. The next day they do 90 minutes of endurance cycling in the laboratory. The initial power is 50W, and the speed of 60rpm is increased by 20W per minute until the corresponding load of ventilation anaerobic threshold appears. Before exercise, venous blood is collected under the condition of empty stomach and quiet. Venous blood was collected at 0 h, 1 h, 4 h and 24 h after exercise. The intensity of anaerobic threshold was 60-90% at intervals of 2 weeks. There were 10 groups of intermittent 4 mins 20-40% anaerobic threshold strength pedaling. The blood collection plan was the same. After two exercise tests, venous blood was collected again one week after the end of the two exercise tests to determine the protein content of CKMb BUNMG53 in serum. All the measured data were carried out by SPSS 22.0 statistical software. Analysis. Results: in endurance exercise of power vehicles at different time points and movements, There was no significant difference in serum CK between different time points (P 0.05). Mb at 1 h and 24 h after exercise was significantly higher than that at 1 h and 24 h after exercise, respectively. The BUN was significantly increased at 1 h and 4 h after exercise, and the BUN at 1 h and 4 h after exercise was quieter than that at 1 h and 4 h after exercise. The BUN of 24 hours after exercise was significantly lower than that of BUN at 1 and 4 hours after exercise. There was no significant difference in MG53 value at different time points (P0.05. 2) in high intensity intermittent exercise. There was no significant difference in serum CK between different time points (P 0.05). Mb at 1 h and 24 h after exercise was significantly higher than that at 1 h and 24 h after exercise. There was no significant difference in BUN between different time points (P 0.05). There was no significant difference in MG53 value at different time points (P 0.05). 4 hours after the exercise, Motor vehicle endurance exercise (Mb) was significantly higher than that of high intensity intermittent exercise (P 0.05U. 2) the BUN of power vehicle endurance exercise was significantly higher than that of high intensity intermittent exercise (P 0.05U. MG53). There was a positive correlation between Mb and CK at 4 hours after exercise, and a positive correlation between Mb and CK at 24 hours after exercise. There was no correlation between Mb and CK at different time points. There was no correlation between CK and Mb at different time points and MG53 had no correlation with CKMb bun at different time points. Conclusion: the endurance exercise of power vehicle and the high intensity intermittent exercise have significant changes. After 4 hours of exercise, the change of serum Mbbun was significant. 3) the content of human MG53 increased immediately after two aerobic exercise modes compared with that before exercise. The content of human MG53 was higher than that before and immediately after aerobic exercise in different ways, and immediately after exercise, the content of human MG53 was increased immediately after exercise. There was no correlation between serum CKB and Mbbun at 1 hour, 4 hours and 24 hours after exercise. (5) the period of change of serum MG53 was different from that of serum CK Mb after aerobic exercise.
【學(xué)位授予單位】：首都體育學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R873

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