發(fā)布時(shí)間：2018-02-25 09:43

  本文關(guān)鍵詞： 頭頸部腫瘤 T細(xì)胞亞群 免疫功能 碳離子束　出處：《現(xiàn)代腫瘤醫(yī)學(xué)》2016年20期 　論文類型：期刊論文

【摘要】：目的:對(duì)比研究碳離子束和X射線對(duì)健康人與頭頸部惡性腫瘤患者離體外周血淋巴細(xì)胞亞群影響,了解不同射線、不同放射治療劑量對(duì)健康人和惡性腫瘤患者離體外周血淋巴細(xì)胞亞群分布的差異。方法:在采血后8小時(shí)內(nèi)分別行6MV-X線1Gy、2Gy照射及~(12+)C離子束1Gy E、2Gy E照射;應(yīng)用美國BD公司的四色熒光標(biāo)記抗人CD分子單克隆抗體和FACSVerse流式細(xì)胞儀,檢測(cè)外周血淋巴細(xì)胞亞群。結(jié)果:各T淋巴細(xì)胞亞群CD3~+、CD4~+、CD8~+、CD4~+/CD8~+及NK細(xì)胞、B細(xì)胞無顯著差異(P0.05),但CD4~+/CD8~+在健康人離體血中下降更為明顯。結(jié)論:對(duì)健康人和頭頸部鱗癌患者的離體外周血行6 MV-X線1 Gy、2 Gy照射及~(12+)C離子束1Gy E、2Gy E照射,兩種射線對(duì)T淋巴細(xì)胞亞群的影響無明顯差異,健康人和頭頸部鱗癌患者間也無明顯差異。結(jié)果對(duì)臨床中放射治療放射方法的選擇具有重要參考價(jià)值。
[Abstract]:Objective: to compare the effects of carbon ion beam and X ray on lymphocyte subsets in peripheral blood of healthy people and patients with malignant tumor of head and neck. The distribution of lymphocyte subsets in peripheral blood of healthy persons and malignant tumor patients was different with different doses of radiotherapy. Methods: within 8 hours after blood collection, 6MV-X ray irradiation of 1 Gy ~ 2Gy and ~ (12) C ~ + beam of 1 Gy E ~ (2 Gy) were carried out respectively. The monoclonal antibody against human CD molecule and FACSVerse flow cytometry were labeled with four colors fluorescence from BD Company of USA. Results: there was no significant difference between CD3 ~ + CD4 ~ + CD8 ~ + CD4 ~ / CD8 ~ + T lymphocyte subsets and NK cell B cells, but CD4 ~ / / CD8 ~ + decreased more obviously in the blood of healthy people in vitro. Conclusion: in healthy people and head and neck, there is no significant difference between them. In vitro peripheral blood of patients with squamous cell carcinoma was irradiated with 1 Gy / 2 Gy of 6 MV-X line and 1 Gy / 2 Gy of E ~ (2 Gy) with ~ (12) C ion beam. There was no significant difference between the two kinds of radiation on T lymphocyte subsets, and there was no significant difference between healthy persons and patients with head and neck squamous cell carcinoma. The results have important reference value for the choice of radiotherapy methods in clinic.
【作者單位】： 甘肅省腫瘤醫(yī)院放療科;中國科學(xué)院近代物理研究所;
【基金】：甘肅省技術(shù)研究與開發(fā)專項(xiàng)計(jì)劃項(xiàng)目(編號(hào):1305TCYA009)
【分類號(hào)】：R739.91;R730.55

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