本文關(guān)鍵詞： 口腔干燥 放射性 模型 動(dòng)物 頜下腺 組織工程 組織構(gòu)建 放射性口干癥 動(dòng)物模型 直線加速器 廣東省自然科學(xué)基金　出處：《中國(guó)組織工程研究》2017年32期 　論文類型：期刊論文

【摘要】：背景:目前對(duì)于唾液腺的放射性損傷機(jī)制還未明確,治療和防護(hù)措施也處于探索階段。目的:建立放射性口干癥大鼠模型,并觀察18 Gy電子線照射后大鼠頜下腺組織損傷情況及唾液成分變化。方法:通過隨機(jī)對(duì)照實(shí)驗(yàn),將115只Wistar大鼠隨機(jī)分成照射組和假照射組,假照射組只麻醉不照射,照射組麻醉后使用直線加速器一次性照射18 Gy。將大鼠頜下腺部位暴露于照射區(qū),制備放射性口干癥模型。記錄造模后21 d內(nèi)飲水量;分1,3,7,14,21,28,35,42 d 8個(gè)時(shí)間點(diǎn)收集唾液、摘取頜下腺腺體,測(cè)量唾液量、頜下腺指數(shù),對(duì)頜下腺切片行蘇木精-伊紅染色觀察組織形態(tài)學(xué)變化。結(jié)果與結(jié)論:(1)放射后1-21 d照射組大鼠每天平均飲水量為(6.42±1.91)m L,假照射組為(4.82±1.20)m L,差異有顯著性意義(P0.05);(2)在放射后42 d內(nèi)照射組唾液量均少于假照射組,其中照射組大鼠照射后第7天唾液量降至最低;7,21,28,42 d比較差異有顯著性意義(P0.05);(3)1 d及21-42 d,照射組頜下腺指數(shù)均小于假照射組(P0.05);(4)蘇木精-伊紅病理結(jié)果顯示,照射組大鼠頜下腺炎性浸潤(rùn)、腺體萎縮、核固縮等損傷情況呈漸進(jìn)性加重,放射后42 d損傷最為嚴(yán)重;(5)綜上,Wistar大鼠接受直線加速器電子線18 Gy照射后,放射性口干癥模型成功建立,直觀表現(xiàn)為飲水量增加,唾液量減少,病理切片顯示頜下腺病理?yè)p傷程度在42 d內(nèi)隨時(shí)間的增加呈進(jìn)行性加重。
[Abstract]:Background: the mechanism of radioactive damage of salivary glands is not clear, and the treatment and protection measures are still in the exploring stage. Objective: to establish a rat model of radiation-induced xerostomia. The changes of submandibular gland tissue and salivary composition in rats after 18 Gy electron irradiation were observed. Methods: 115 Wistar rats were randomly divided into irradiation group and false irradiation group. After anesthesia, the rats in the irradiation group were exposed to the irradiation area with a linear accelerator for 18 Gy.The rats were exposed to the irradiation area to make a model of radioorrhagia. The drinking water was recorded within 21 days after the model was made, and saliva was collected at 8 time points of 1: 3, 71421, 2828, 3542 days, respectively, after the exposure of the submandibular gland of the rats to the irradiation area. The submandibular gland was removed, salivary volume was measured, and submandibular gland index was measured. The histological changes were observed by hematoxylin-eosin staining on submandibular gland sections. Results and conclusions the average daily drinking water of rats in 1-21 d radiation group was 6. 42 鹵1. 91 mL, and that in sham-irradiated group was 4. 82 鹵1. 20 mL. the difference was significant (P 0. 05 2). The amount of saliva in the irradiation group was less than that in the false irradiation group within 42 days after irradiation. The salivary volume of rats in the irradiation group decreased to the lowest level on the 7th day after irradiation. There was a significant difference in saliva volume between the two groups on the 7th day after irradiation. There was a significant difference between the two groups on the 1st day and 21-42 d after irradiation. The index of submandibular gland in the irradiation group was lower than that in the sham irradiation group (P 0.05). The pathological results of hematoxylin and eosin showed that the submandibular gland index in the irradiated group was lower than that in the sham irradiation group. The inflammatory infiltration of submandibular gland, atrophy of glands and nuclear pyknosis were gradually aggravated in the irradiation group, and the damage was the most serious at 42 days after irradiation. The model of xerostomia was established successfully. The visual manifestation was that the quantity of drinking water increased and the amount of saliva decreased. The pathological section showed that the degree of pathological injury of submandibular gland increased progressively with the increase of time within 42 days.
【作者單位】： 廣州中醫(yī)藥大學(xué)護(hù)理學(xué)院;廣東省中醫(yī)院大學(xué)城放療科;
【基金】：廣東省自然科學(xué)基金項(xiàng)目(2014A030313405)~~
【分類號(hào)】：R-332;R730.55
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本文編號(hào)：1495409