本文關(guān)鍵詞：80Hz低頻電刺激早期治療股二頭肌急性拉傷實驗研究　出處：《中國運動醫(yī)學(xué)雜志》2016年04期 　論文類型：期刊論文

  更多相關(guān)文章： 股二頭肌 急性拉傷 電刺激

【摘要】：目的:采用80 Hz低頻電刺激早期治療股二頭肌急性拉傷,觀察其對肌肉損傷部位結(jié)構(gòu)重塑和功能的影響。方法:SD雄性大鼠36只,先按體重分層,再隨機(jī)分為正常對照組和實驗組,后者依據(jù)不同實驗取材時間分為D0組、D7組、D7-80 Hz、D14組和D14-80 Hz組,每組6只。實驗組大鼠實施麻醉,采用針電刺激使股二頭肌強直收縮,同時膝關(guān)節(jié)角度從屈曲70°到完全伸直180°以角速度960°·s-1伸膝擺腿反向拉伸,建立股二頭肌急性拉傷動物模型。造模后D7-80Hz組和D14-80 Hz組于動物造模后第5天開始給予80 Hz電刺激治療(2次/日,30分鐘/次,間隔4小時),其余組無特殊干預(yù)繼續(xù)喂養(yǎng)。正常對照組、D0組、D7組、D7-80 Hz組、D14組和D14-80 Hz組分別在第0、0、7、7、14、14天進(jìn)行在體力矩測試,觀測膝關(guān)節(jié)力矩變化以及力矩 角度關(guān)系。實驗結(jié)束后處死動物,分離股二頭肌,液氮固定、OCT包埋,組織切片、HE染色,光鏡下觀察股二頭肌組織形態(tài)。結(jié)果:光鏡觀察可見肌肉拉傷當(dāng)天肌纖維壞死,肌肉組織結(jié)構(gòu)紊亂,肌纖維水腫變性,肌肉間隙大小不一。拉傷后第7天D7組觀察可見,肌纖維雖然有所修復(fù),但是形態(tài)不成熟,大小不一,排列不嚴(yán)整。經(jīng)過80 Hz電刺激治療,肌肉組織結(jié)構(gòu)并沒有明顯改善。力矩測試D0組關(guān)節(jié)最大等長力矩值(0.246±0.026 Nm)下降明顯,與其余各組比較均有顯著性差異(P0.05);D7-80 Hz組(0.302±0.033 Nm)和D14-80 Hz組(0.334±0.060 Nm)關(guān)節(jié)最大等長力矩值分別與D7組和D14組比較均無顯著性差異(P0.05)。正常對照組最優(yōu)角度為130.00°±3.54°,經(jīng)80 Hz電刺激治療后D14-80 Hz組力矩 角度關(guān)系曲線右偏移,關(guān)節(jié)最優(yōu)角度為121.00°±4.18°,與D14組(120.00°±3.53°)比較并沒有顯著性差異(P0.05)。結(jié)論 :股二頭肌急性拉傷后早期采用80Hz低強度電刺激治療,2周時間內(nèi)并不能有效地促進(jìn)損傷肌肉結(jié)構(gòu)重塑,也不能提高關(guān)節(jié)最大等長力矩以及優(yōu)化關(guān)節(jié)力矩 角度關(guān)系。
[Abstract]:Objective: to observe the effect of 80 Hz low frequency electric stimulation on the structural remodeling and function of biceps femoris muscle injury in early stage. Methods: 36 male SD rats were divided according to their body weight. Then they were randomly divided into normal control group and experimental group, the latter was divided into D0 group, D7 group and D14 group and D14-80 Hz group according to the time of sampling. Six rats in each group were anesthetized and the biceps femoris muscle was constricted by electroacupuncture. At the same time, the knee joint angle from flexion 70 擄to complete elongation 180 擄with 960 擄路s ~ (-1) extension knee swing leg reverse stretching. The animal model of acute strain injury of biceps femoris was established. The rats in D7-80Hz group and D14-80Hz group were treated with 80 Hz electric stimulation twice a day on the 5th day after the establishment of the model. The rest group was fed continuously without special intervention. The normal control group was treated with D0 group and the D7 group was treated with D7-80 Hz. D14 group and D14-80 Hz group were tested for in vivo torque on day 14 respectively. At the end of the experiment, the animals were killed, the biceps femoris were separated, the Oct was fixed with liquid nitrogen, and the tissue sections were stained with HE. Results: muscle fiber necrosis, muscle tissue structure disorder, muscle fiber edema and degeneration were observed under light microscope. The size of the muscle space was different. The D7 group was observed on the 7th day after the injury. Although the muscle fibers were repaired, they were immature in shape, different in size and not well arranged, and were treated with 80 Hz electrical stimulation. There was no significant improvement in muscle structure. The maximum isometric torque value of joint in D0 group was 0.246 鹵0.026 Nm). Compared with other groups, there was a significant difference (P 0.05). D7-80 Hz (0.302 鹵0.033 Nm) and D14-80 Hz (0.334 鹵0.060 Nm). There was no significant difference in joint maximum equal length moment between D7 group and D14 group (P 0.05). The optimal angle of normal control group was 130.00 擄鹵3.54 擄. After 80 Hz electric stimulation, the angle curve of D14-80 Hz group was shifted to the right, the optimal angle of joint was 121.00 擄鹵4.18 擄. There was no significant difference between D14 group (120.00 擄鹵3.53 擄) and D14 group (P0.050.Conclusion: 80 Hz low intensity electric stimulation was used in the early stage after acute strain injury of biceps femoris muscle. In 2 weeks, it can not effectively promote the remodeling of injured muscle structure, nor can it improve the maximum equal length moment of joint and optimize the angle relationship of joint torque.
【作者單位】： 海南熱帶海洋學(xué)院體育與休閑學(xué)院;國家體育總局運動醫(yī)學(xué)研究所;
【基金】：廣東省自然科學(xué)基金(10451007601004461)
【分類號】：R873
【正文快照】： 肌肉拉傷是運動中最常見的損傷之一。目前電刺激在臨床治療肌肉損傷中廣為應(yīng)用,其有效性和安全性不斷得到臨床實踐的證實。依據(jù)低頻電刺激治療的目的,電刺激頻率變化范圍可以很大,在臨床治療中10~100 Hz較為常用[1,2]。我們的研究證實急性拉傷后早期采用20 Hz低頻電刺激能夠產(chǎn)

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