【摘要】：目的 調(diào)查煤焦瀝青職業(yè)接觸人群p16、FHIT和RASSF1A基因啟動(dòng)子甲基化及端粒損傷情況,探討煤焦瀝青職業(yè)接觸的效應(yīng)標(biāo)志。 對(duì)象與方法 1.研究對(duì)象：180例煤焦瀝青職業(yè)接觸人群來(lái)自中部某碳素廠職工；145例對(duì)照人群來(lái)自同期體檢正常的人群,此人群都是在本學(xué)校第一附屬醫(yī)院體檢科進(jìn)行體檢。經(jīng)其知情同意后,采集研究對(duì)象外周血,專業(yè)調(diào)查人員收集研究對(duì)象基本信息。 2.實(shí)驗(yàn)方法：外周血DNA相對(duì)端粒長(zhǎng)度采用實(shí)時(shí)熒光定量PCR的方法進(jìn)行分析。p16、RASSF1A和FHIT基因甲基化水平采用實(shí)時(shí)熒光定量甲基化特異性PCR的方法進(jìn)行分析。 3.統(tǒng)計(jì)學(xué)分析：根據(jù)定量資料分布類型選擇表示方法和統(tǒng)計(jì)學(xué)檢驗(yàn)方法。定性資料比較采用用χ2檢驗(yàn)。檢驗(yàn)水準(zhǔn)α=0.05。 結(jié)果 1.結(jié)果顯示煤焦瀝青職業(yè)暴露人群端粒長(zhǎng)度和對(duì)照組端粒長(zhǎng)度分別為0.83(0.71-0.90)和1.13(0.72-1.45),兩組比較差異有統(tǒng)計(jì)學(xué)意義(Z=-5.395,P0.001)。 2.職業(yè)暴露組和對(duì)照組p16基因啟動(dòng)子甲基化率分別為0.56(0.13-4.95)和0.37(0.06-4.23),兩組差異無(wú)統(tǒng)計(jì)學(xué)意義(Z=-1.395,P=0.163)；職業(yè)暴露組和對(duì)照組FHIT啟動(dòng)子甲基化率分別為3.22(2.09-4.64)和2.95(1.45-5.88),兩組差異無(wú)統(tǒng)計(jì)學(xué)意義(Z=-0.906,P=0.365)；職業(yè)暴露組和對(duì)照組RASSF1A基因啟動(dòng)子甲基化率分別為14.76(7.26-39.09)和19.34(3.82-50.95),兩組差異無(wú)統(tǒng)計(jì)學(xué)意義(Z=-0.455,P=0.649)。 結(jié)論 煤焦瀝青職業(yè)暴露可引起人外周血白細(xì)胞DNA端粒長(zhǎng)度縮短；煤焦瀝青職業(yè)暴露不能導(dǎo)致p16、RASSF1A和FHIT基因啟動(dòng)子甲基化率改變。
[Abstract]:Objective to investigate the promoter methylation and telomere damage of p16 FHIT and RASSF1A gene in occupational exposure population of coal tar pitch, and to explore the effect markers of occupational exposure of coal tar pitch. Object and method 1. Participants: one hundred and eighty cases of occupational contact with coal tar pitch were collected from 145 workers of a carbon factory in the central part of the university. All of them were examined in the physical examination department of the first affiliated hospital of our school. After informed consent, the subjects were collected peripheral blood, professional investigators to collect basic information. 2. 2. Methods: the relative telomere length of peripheral blood DNA was analyzed by real-time fluorescence quantitative PCR. The methylation level of p16 RASSF1A and FHIT gene was analyzed by real-time fluorescence quantitative methylation specific PCR. 3. Statistical analysis: according to the distribution type of quantitative data, the representation method and statistical test method were selected. The qualitative data were compared by 蠂 2 test. The test level is 偽 0. 05. Result 1. The results showed that telomere length was 0.83 (0.71-0.90) in occupational exposed population and 1.13 (0.72-1.45) in control group. The promoter methylation rate of p16 gene was 0.56 (0.13-4.95) and 0.37 (0.06-4.23) in occupational exposure group and control group, respectively, and there was no significant difference between the two groups (ZP0.163), and the methylation rate of FHIT promoter in occupational exposure group and control group was 3.22 (2.09-4.64) and 2.95 (1.45-5.88), respectively. The methylation rates of RASSF1A gene promoter in occupational exposure group and control group were 14.76 (7.26-39.09) and 19.34 (3.82-50.95), respectively. Conclusion Occupational exposure to coal tar pitch can shorten the length of DNA telomere in human peripheral blood leukocytes, while occupational exposure of coal tar pitch does not change the methylation rate of p16 RASSF1A and FHIT gene promoter.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R131

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