【摘要】：【研究目的】 評(píng)價(jià)海洋石油作業(yè)工人的職業(yè)緊張水平，了解血管緊張素原（AGT）和神經(jīng)調(diào)節(jié)因子（NRG1）的基因多態(tài)性，分析兩基因各基因型與職業(yè)緊張分級(jí)的關(guān)系，探討確定AGT和NRG1基因是否為職業(yè)緊張的易感基因。 【研究方法】 問(wèn)卷調(diào)查：抽取勝利海洋采油廠包括EDC平臺(tái)在內(nèi)作業(yè)崗位的300名在崗員工，采用常規(guī)的職業(yè)緊張量表，進(jìn)行問(wèn)卷調(diào)查。問(wèn)卷使用1998年修訂版的中文版職業(yè)緊張量表，調(diào)查方式采用面對(duì)面方式，調(diào)查內(nèi)容包括一般情況、飲食及生活習(xí)慣、家族史、疾病史、職業(yè)情況（如工齡、工種）和職業(yè)緊張狀況。調(diào)查結(jié)果使用EpiData建庫(kù)，用SPSS軟件進(jìn)行統(tǒng)計(jì)分析，得出采油廠工人職業(yè)緊張情況，并分析婚姻、學(xué)歷，年齡和工齡等因素對(duì)職業(yè)緊張情況的影響。 實(shí)驗(yàn)驗(yàn)證：在取得工人知情同意的情況下，抽取上述工人靜脈血，共300份。使用DNA快提試劑盒，提取工人DNA，設(shè)計(jì)AGT和NRG1兩個(gè)基因的引物，使用PCR-RFLP法分析兩基因的基因多態(tài)性。最后結(jié)合問(wèn)卷調(diào)查結(jié)果，統(tǒng)計(jì)分析找出不同基因型對(duì)職業(yè)緊張指標(biāo)的影響。 【研究結(jié)果】 問(wèn)卷調(diào)查 1）緊張總體情況 本次調(diào)查對(duì)象職業(yè)任務(wù)緊張情況相對(duì)樂(lè)觀，個(gè)體緊張反應(yīng)表現(xiàn)在心理緊張反應(yīng)和軀體緊張反應(yīng)兩個(gè)方面；在應(yīng)對(duì)能力上，社會(huì)支持和理性處事是主要弱項(xiàng)。本次調(diào)查對(duì)象在職業(yè)任務(wù)和應(yīng)對(duì)能力方面均優(yōu)于常模。 2）職業(yè)緊張影響因素方面 本次研究從婚姻狀況、學(xué)歷情況、吸煙飲酒情況和年齡、工齡六個(gè)方面分析職業(yè)緊張的影響因素，發(fā)現(xiàn)不同年齡段對(duì)于緊張反應(yīng)的應(yīng)對(duì)能力不同，25到30歲的年齡段對(duì)于緊張的應(yīng)對(duì)能力最差，吸煙飲酒情況可能會(huì)對(duì)職業(yè)緊張某些要素發(fā)揮作用，，婚姻情況和學(xué)歷的高低則未表現(xiàn)出有意義的差異。 3）多元回歸分析 通過(guò)多元回歸分析發(fā)現(xiàn)，社會(huì)支持和責(zé)任感是對(duì)個(gè)人緊張反應(yīng)影響最普遍的兩個(gè)因素，社會(huì)支持與緊張反應(yīng)呈負(fù)相關(guān)，社會(huì)支持度的提高可以極大緩解工人個(gè)體緊張反應(yīng)情況，研究者在設(shè)計(jì)職業(yè)緊張干預(yù)措施時(shí)，應(yīng)予以重點(diǎn)考慮。 實(shí)驗(yàn)驗(yàn)證 1）AGT和NRG1的基因多態(tài)性 AGT基因經(jīng)過(guò)電泳后得出三個(gè)基因型，即：純合子MM型（163bp）、TT型（140bp）和雜合子MT型（140bp、163bp）；NRG1基因經(jīng)過(guò)電泳后也得出三各基因型，即：純合子TT型（30bp、127bp）、GG型（60bp、127bp）和雜合子TG型（30bp、60bp、127bp）。經(jīng)過(guò)檢測(cè)，他們均符合遺傳學(xué)Hardy-Weinberg平衡（P＞0.05），提示研究對(duì)象無(wú)偏倚。 2）職業(yè)緊張易感性 綜合分析問(wèn)卷和實(shí)驗(yàn)結(jié)果發(fā)現(xiàn)，血管緊張素原（AGT）和神經(jīng)調(diào)節(jié)因子（NRG1）這兩個(gè)基因的不同基因型攜帶者職業(yè)緊張指標(biāo)之間均有顯著的統(tǒng)計(jì)學(xué)差異(P＜0.05)，攜帶AGT(M235T M/T位點(diǎn))T等位基因和NRG1(rs3924999G/T位點(diǎn))T等位基因的研究對(duì)象職業(yè)任務(wù)陽(yáng)性率和緊張反應(yīng)陽(yáng)性率顯著高于AGT(M235T M/T位點(diǎn))M等位基因和NRG1(rs3924999G/T位點(diǎn))G等位基因的基因型攜帶者。 【研究結(jié)論】 1）當(dāng)前海洋采油廠工人的個(gè)體緊張反應(yīng)主要表現(xiàn)在心理緊張反應(yīng)和軀體緊張反應(yīng)方面，社會(huì)支持和理性處事是研究對(duì)象應(yīng)對(duì)緊張的弱項(xiàng)，然而通過(guò)回歸分析來(lái)看，這兩項(xiàng)的重要程度不言而喻，特別是社會(huì)支持一項(xiàng)，將會(huì)很大程度上影響工人的職業(yè)緊張應(yīng)對(duì)能力。 2）血管緊張素原AGT(M235T M/T位點(diǎn))T等位基因和神經(jīng)調(diào)節(jié)因子NRG1(rs3924999G/T位點(diǎn))T等位基因可能是職業(yè)緊張的易感基因。
[Abstract]:[research purposes]
To evaluate the occupational stress level of offshore oil workers, to understand the genetic polymorphisms of angiotensinogen (AGT) and neuroregulatory factor (NRG1), to analyze the relationship between the two genotypes and occupational stress grading, and to determine whether AGT and NRG1 genes are susceptible to occupational stress.
[research methods]
Questionnaire survey: 300 on-the-job employees in Shengli offshore oil production plant, including EDC platform, were surveyed with the Occupational Stress Scale. The questionnaire used the Chinese version of Occupational Stress Scale revised in 1998. The survey was conducted face-to-face. The contents of the survey included general situation, diet and living habits, and family. Family history, disease history, occupational status (e.g. length of service, type of work) and occupational stress were analyzed by using EpiData database and SPSS software.
The results showed that 300 venous blood samples were obtained with the informed consent of the workers. The DNA of the workers was extracted by DNA rapid extraction kit, and the primers of AGT and NRG1 genes were designed. The polymorphisms of the two genes were analyzed by PCR-RFLP. Finally, the results of the questionnaire survey were used to analyze and find out the occupational stress caused by different genotypes. The influence of Zhang index.
[results]
Questionnaire investigation
1) the general situation of tension.
The respondents were relatively optimistic about occupational task stress, and their individual stress reaction was manifested in two aspects: psychological stress reaction and physical stress reaction. Social support and rational behavior were the main weaknesses in coping ability. The respondents were superior to the norm in occupational task and coping ability.
2) factors affecting occupational stress
This study analyzed the influencing factors of occupational stress from six aspects: marital status, educational background, smoking and drinking, age and length of service. It was found that different age groups had different coping abilities to stress reaction, the age group between 25 and 30 had the worst coping ability to stress, and smoking and drinking might exert some factors of occupational stress. There was no significant difference in marital status and educational background.
3) multiple regression analysis
Through multiple regression analysis, it is found that social support and sense of responsibility are the two most common factors affecting individual stress response. Social support is negatively correlated with stress response, and the improvement of social support can greatly alleviate individual stress response of workers.
experimental verification
1) genetic polymorphisms of AGT and NRG1
After electrophoresis, three genotypes of AGT gene were identified: homozygote MM (163 bp), TT (140 bp) and heterozygote MT (140 bp, 163 bp), and NRG1 gene was also identified as homozygote TT (30 bp, 127 bp), GG (60 bp, 127 bp) and heterozygote TG (30 bp, 60 bp, 127 bp). Inberg balance (P > 0.05) indicated that the subjects were not biased.
2) occupational stress and susceptibility
The results of questionnaire and experiment showed that there were significant differences in occupational stress between the carriers of angiotensinogen (AGT) and neuroregulatory factor (NRG1) genotypes (P The task-positive rate and the stress-positive rate were significantly higher than those of the genotype carriers of AGT (M235T M/T locus) M allele and NRG1 (rs3924999G/T locus) G allele.
[Conclusion]
1) At present, the individual stress reaction of the workers in offshore oil production plants is mainly manifested in the aspects of psychological stress reaction and physical stress reaction. Social support and rational behavior are the weaknesses of the research objects in dealing with stress. However, through regression analysis, the importance of these two factors is self-evident, especially social support, which will greatly affect. Workers' occupational stress coping ability.
2) Angiotensinogen AGT (M235T M/T locus) T allele and neuromodulator NRG1 (rs3924999G/T locus) T allele may be susceptible to occupational stress.
【學(xué)位授予單位】：濟(jì)南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R131

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