【摘要】：目的:通過采用小鼠淋巴瘤細(xì)胞實(shí)驗(yàn)微孔法,檢測二甲基亞砜(DMSO)的細(xì)胞毒性及遺傳毒性,探索其在檢測系統(tǒng)中的適宜濃度。方法:在非代謝活化(-S9)條件下,檢測美國Sigma和Tedi A兩家公司生產(chǎn)的2個(gè)批號DMSO,終濃度0.5%,1.0%,2.0%,4.0%處理L5178Y細(xì)胞3 h后產(chǎn)生的細(xì)胞毒性及誘發(fā)的TK基因突變頻率(MF)。結(jié)果:2個(gè)批號DMSO的細(xì)胞毒性均以1%濃度組為最低(20%),4%濃度組為最高(37%);1%濃度組誘發(fā)的MF與空白對照組相近,而其他3組則明顯增加(P0.05或0.01)。結(jié)論:當(dāng)以DMSO為溶媒、采用L5178Y細(xì)胞進(jìn)行MLA微孔法實(shí)驗(yàn)時(shí),采用1%作為檢測系統(tǒng)中受試物的體積比濃度較為適宜。
[Abstract]:Aim: to detect the cytotoxicity and genetic toxicity of dimethyl sulfoxide (DMSO) by micropore assay in mouse lymphoma cells and to explore the appropriate concentration of dimethyl sulfoxide (DMSO) in the detection system. Methods: under the condition of non-metabolic activation (-S9), the final concentration of two batches of DMSO, produced by Sigma and Tedi A in the United States was determined. The cytotoxicity of L5178Y cells and the induced TK gene mutation frequency (MF). Were detected after 3 h treatment of L5178Y cells with 1.0% or 2.0% DMSO,. Results: the cytotoxicity of the two batches of DMSO was the lowest (20%) in the 1% concentration group and the highest (37%) in the 4% concentration group (37%). The MF induced by the two batches of DMSO was similar to that of the blank control group, while the other three groups were significantly increased (P0.05 or 0.01). Conclusion: when L5178Y cells were used for MLA micropore assay with DMSO as solvent, the volume ratio of 1% was more suitable for the detection system.
【作者單位】： 中國食品藥品檢定研究院國家藥物安全評價(jià)監(jiān)測中心;北京市藥品檢驗(yàn)所;安徽省食品藥品檢驗(yàn)所;
【分類號】：R114

【參考文獻(xiàn)】

相關(guān)期刊論文 前3條

1 黃波;陳鋒;朱茂祥;楊陟華;張蓉芳;;二甲基亞砜拮抗烹調(diào)油煙冷凝物遺傳毒性的研究[J];環(huán)境與健康雜志;2007年03期

2 曹陽，，胡維民,曹佳,許莉萍;二甲基亞砜在λDNA重包裝法中的遺傳毒性研究[J];衛(wèi)生毒理學(xué)雜志;1996年01期

3 趙潔;胡燕平;宋捷;張e

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