【摘要】：目的 1、通過對研究對象呼吸和飲食途徑攝入PAHs的水平進(jìn)行分析,分析季節(jié)對呼吸和飲食攝入PAHs的影響及兩種途徑攝入PAHs的相對比例并評估兩種途徑攝入PAHs對研究對象所致的健康風(fēng)險,確定兩種途徑對人體健康危害的程度,以采取相應(yīng)的措施保護(hù)人群健康。 2、通過對尿中OH-PAHs和8-OHdG的測定,尋找全面而準(zhǔn)確地評價PAHs內(nèi)暴露生物標(biāo)志物,并探討在一般人群中用8-OHdG反映PAHs暴露所致早期遺傳損傷的可行性。 方法 用GC-MS分析顆粒物和食物中PAHs的水平及尿中OH-PAHs的水平,用ELISA分析尿中8-OHdG的水平；用兩獨立樣本Mann-Whitney U檢驗分析季節(jié)對顆粒物和食物中PAHs含量的影響,用蒙特卡洛模擬評估呼吸和飲食途徑攝入PAHs的致癌風(fēng)險。用Spearman函數(shù)分析OH-PAHs間及和8-OHdG間的相關(guān)性,用歐氏距離法分析OH-PAHs與顆粒物及食物中其原型化合物間的相關(guān)性,用多元線性回歸分析PAHs對8-OHdG的影響。 結(jié)果 1.季節(jié)對顆粒物中16種PAHs的影響差異均具有統(tǒng)計學(xué)意義(P0.05),且低環(huán)PAHs夏季高于冬季,高環(huán)PAHs冬季高于夏季；季節(jié)對食物PAHs中Aci、Flu、 BbFlu、Ant、Chr和BkFlu的影響差異具有統(tǒng)計學(xué)意義(P0.05),且除了Aci外均為夏季高于冬季。夏季呼吸途徑PAHs暴露所占比例為2%,飲食途徑所占比例為98%；冬季呼吸途徑所占比例為5%,飲食途徑所占比例為95%。 2.尿中OH-PAHs的濃度按照1-OHNap2-OHNap9-OHFlu2-OHFlu3-OHPhe2-OHPhe3-OHFlu4-OHPhe1-OHPyr2-OHBcPhe6-OHChr的順序遞減,尿中OH-PAHs以低環(huán)的Nap、Flu和Phe為主。1-OHPyr與其它OH-PAHs間顯著相關(guān),rs為0.454-0.902(P0.01)。各種OH-PAHs與顆粒物載帶的其原型化合物之間的相關(guān)性按照1-OHPyr6-OHChr2-OHNap2-OHFlu2-OHBcPhe的順序遞減；與食物中其原型化合物之間的相關(guān)性按照1-OHPyrΣ OHNap6-OHChr2-OHBcPhe2-OHFlu的順序遞減。除1-OHNap和2-OHBcPhe外,其余9種OH-PAHs與8-OHdG間的相關(guān)性均具有統(tǒng)計學(xué)意義(P0.05)。 3.單因素分析PAHs、1-OHNap、2-OHBcPhe、年齡、性別、吸煙、飲酒和鍛煉對8-OHdG水平的影響無統(tǒng)計學(xué)意義,BMI及其他9種OH-PAHs對8-OHdG水平的影響有統(tǒng)計學(xué)意義(P0.05),且8-OHdG隨BMI的減少及OH-PAHs的增加而增加；經(jīng)多元線性回歸分析未發(fā)現(xiàn)OH-PAHs和BMI對尿中8-OHdG水平有影響。 4.呼吸PAHs暴露研究對象ILCR夏季和冬季分別為-2.37×10"8～2.53×10-6和-1.81×10-6_1.78×10-,夏季和冬季呼吸PAHs暴露的ILCR均值分別為1.70×10-7和2.16x10-6；飲食PAHs暴露夏季和冬季的ILCR分別為2.27×10"8-7.48×10-5和4.87×10-1～1.42x10-5,夏季和冬季ILCR的均值分別為2.60×10-6和1.34×10-6。 結(jié)論 1.夏季飲食PAHs暴露的致癌風(fēng)險略高于呼吸PAHs暴露的致癌風(fēng)險,而冬季呼吸PAHs暴露的致癌風(fēng)險略高于飲食PAHs暴露的風(fēng)險,但都在可接受的風(fēng)險水平內(nèi)。 2.可以將尿中的2-OHNap、2-OHFlu和2-OHBcPhe結(jié)合1-OHPyr更為準(zhǔn)確和全面的反映呼吸和飲食途徑PAHs總體內(nèi)暴露水平；可將8-OHdG作為一般人群PAHs暴露早期遺傳毒性效應(yīng)的敏感生物標(biāo)志物。
[Abstract]:objective
1. By analyzing the level of PAHs intake by respiratory and dietary pathways, the influence of season on PAHs intake by respiration and diet and the relative proportion of PAHs intake by two pathways were analyzed, and the health risk of PAHs intake by two pathways was assessed to determine the degree of harm of two pathways to human health, so as to adopt corresponding measures. Measures to protect people's health.
2. Through the determination of urinary OH-PAHs and 8-OHdG, to find a comprehensive and accurate assessment of PAHs exposure biomarkers, and to explore the feasibility of using 8-OHdG in the general population to reflect the early genetic damage caused by PAHs exposure.
Method
The levels of PAHs in particulate matter and food were analyzed by GC-MS, and the levels of OH-PAHs in urine were analyzed by ELISA. The effects of seasons on the contents of PAHs in particulate matter and food were analyzed by Mann-Whitney U test with two independent samples, and the carcinogenic risk of PAHs intake by respiratory and dietary pathways was assessed by Monte Carlo simulation. The correlation between OH-PAHs and 8-OHdG was analyzed. The correlation between OH-PAHs and particulate matter and their prototype compounds in food was analyzed by Euclidean distance method. The effect of PAHs on 8-OHdG was analyzed by multiple linear regression.
Result
1. Seasonal effects on 16 PAHs in particulate matter were statistically significant (P 0.05), and low-ring PAHs were higher in summer than in winter, and high-ring PAHs were higher in winter than in summer. Seasonal effects on Aci, Flu, BbFlu, Ant, Chr and BkFlu in food PAHs were statistically significant (P 0.05), and they were higher in summer than in winter except Aci. S exposure accounted for 2%, dietary pathway accounted for 98%, winter respiratory pathway accounted for 5%, dietary pathway accounted for 95%.
2. The concentration of OH-PAHs in urine decreases according to the sequence of 1-OHNap2-OHNap9-OHFlu2-OHFlu3-OHPhe2-OHPhe3-OHFlu4-OHPhe1-OHPyr2-OHBcPhe6-OHChr. OH-PAHs in urine are mainly low-ring Nap, Flu and Phe. 1-OHPyr are significantly correlated with other OH-PAHs, and the correlation between OH-PAHs and their prototype compounds is 0.454-0.902 (P.01). The correlation decreases in the order of 1-OHPyr 6-OHChr 2-OHNap 2-OHFlu 2-OHBcPhe, and decreases in the order of 1-OHPyr_OHNap 6-OHChr 2-OHBcPhe 2-OHFlu. Except for 1-OHNap and 2-OHBcPhe, the correlation between the other nine OH-PAHs and 8-OHdG is statistically significant (P 0.05).
3. Univariate analysis showed that PAHs, 1-OHNap, 2-OHBcPhe, age, sex, smoking, drinking and exercise had no significant effect on the level of 8-OHdG, BMI and other nine kinds of OH-PAHs had significant effect on the level of 8-OHdG (P 0.05), and 8-OHdG increased with the decrease of BMI and the increase of OH-PAHs; Multiple linear regression analysis showed that OH-PAHs and BMI had no effect on the level of 8-OHdG. Urinary 8-OHdG levels were affected.
4. ILCR in summer and winter were - 2.37 *10"8-2.53 *10-6 and - 1.81 *10-6 1.78 10 -, respectively, and the mean ILCR in summer and winter were 1.70 10-7 and 2.16 10-6, respectively. ILCR in summer and winter were 2.27 10" 8-7.48 10-5 and 4.87 10-1.42 10-5, respectively, and ILCR in summer and winter were 2.27 10 10 10 5 and 4.87 The mean values were 2.60 x 10-6 and 1.34 x 10-6., respectively.
conclusion
1. The carcinogenic risk of dietary PAHs exposure in summer was slightly higher than that of respiratory PAHs exposure, while the carcinogenic risk of winter respiratory PAHs exposure was slightly higher than that of dietary PAHs exposure, but both were within acceptable risk levels.
2. Urinary 2-OHNap, 2-OHFlu and 2-OHBcPhe can be combined with 1-OHPyr to more accurately and comprehensively reflect the overall level of PAHs exposure in the respiratory and dietary pathways; 8-OHdG can be used as a sensitive biomarker for the early genetic toxicity of PAHs exposure in the general population.
【學(xué)位授予單位】：天津醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R114

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