【摘要】：目的建立低硒大鼠模型,比較其對(duì)富硒大米和亞硒酸鈉的生物利用效果。方法 81只3-4周齡雄性SD大鼠隨機(jī)分為正常對(duì)照組和低硒模型組,建立低硒大鼠模型。將低硒大鼠隨機(jī)均分為:低硒模型組,低、高水平亞硒酸鈉組,低、高水平富硒大米組,飼喂相應(yīng)飼料;8周后處死,測(cè)定全血及組織硒含量,血清、肝、腎中GSH-PX、SOD活性和TrxR、MDA含量。結(jié)果 (1)與低硒模型組相比,各富硒組全血及組織硒含量,血清、肝、腎GSH-PX、SOD活性及TrxR含量均升高,MDA含量降低(P0.001)。(2)心、肝硒含量,肝TrxR含量:高水平富硒大米組高于其他富硒組(P0.05);血清MDA含量:高水平富硒大米組低于其他富硒組(P0.05);肝腎GSH-PX及肝SOD活性:2個(gè)高硒組分別高于2個(gè)低硒組(P0.05);肝臟MDA含量:2個(gè)高硒組分別低于2個(gè)低硒組(P0.05);腎臟硒含量、血清TrxR含量:高水平富硒大米組高于低水平富硒大米組。結(jié)論富硒大米生物利用效果優(yōu)于亞硒酸鈉,硒含量高的富硒大米有助于提升低硒模型大鼠抗氧化能力。
[Abstract]:Objective to establish a rat model of low selenium and compare the bioavailability of selenium-enriched rice and sodium selenite. Methods 81 male SD rats aged 3-4 weeks were randomly divided into normal control group and low selenium model group. The rats were randomly divided into three groups: low selenium model group, low and high level sodium selenite group, low and high level selenium enriched rice group. The rats were killed after feeding with the corresponding diet for 8 weeks. The contents of selenium in whole blood and tissue, SOD activity in serum, liver and kidney and the content of TrxRX MDA in serum, liver and kidney were determined. Results (1) compared with the low selenium model group, the contents of selenium in blood and tissue, SOD activity and TrxR content in serum, liver and kidney were all increased in each se enriched group. The contents of TrxR in heart and liver were decreased (P0. 001). (2), and the content of selenium in liver was decreased (P 0. 001). (2). Liver TrxR content: high level selenium-enriched rice group was higher than other selenium-enriched rice group (P0.05); serum MDA content: high level selenium-enriched rice group was lower than other selenium-enriched rice group (P0.05); liver and kidney GSH-PX and liver SOD activity: two selenium-enriched rice groups were higher than two selenium-rich group (P0.05); liver MDA content was higher than that of liver selenium-enriched rice group (P0.05). Amount: 2 high selenium groups were lower than 2 low selenium groups (P0.05), kidney selenium content, The content of serum TrxR was higher in high level se rich rice group than in low level selenium rich rice group. Conclusion Se-enriched rice is more effective than sodium selenite in biological utilization, and high selenium content of selenium-enriched rice is helpful to enhance the antioxidant ability of rats with low selenium.
【作者單位】： 四川大學(xué)華西公共衛(wèi)生學(xué)院(華西第四醫(yī)院);四川省食品藥品檢驗(yàn)檢測(cè)院;中國(guó)農(nóng)業(yè)科學(xué)院生物技術(shù)研究所;成都市溫江區(qū)婦幼保健院;
【基金】：公益性行業(yè)(農(nóng)業(yè))科研專項(xiàng)經(jīng)費(fèi)資助(201303106)
【分類號(hào)】：R151

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本文編號(hào)：2175022