吡草醚原藥大鼠慢性毒性與致癌試驗(yàn)病理研究
發(fā)布時(shí)間：2018-08-09 17:14
【摘要】：吡草醚是一種能夠特異性抑制原卟啉原Ⅸ氧化酶的除草劑，在動(dòng)植物間有良好的選擇性。吡草醚是需光型除草劑，葉面施用后，在光的作用下，能快速誘導(dǎo)植物葉子的壞死或者干枯。迄今為止，，吡草醚是麥田除草劑中活性最高使用范圍最廣的品種之一，因此對(duì)其用藥安全性進(jìn)行系統(tǒng)研究是非常必要的。本文從病理組織學(xué)的角度探討吡草醚原藥對(duì)大鼠的慢性毒性與致癌作用。 按照GB15670-1995《農(nóng)藥登記毒理學(xué)試驗(yàn)方法》進(jìn)行大鼠慢性毒性與致癌性合并試驗(yàn)，將640只大鼠按動(dòng)物體重隨機(jī)分為4組，1個(gè)空白對(duì)照組及低、中、高3個(gè)劑量組，每組160只，雌雄各半。持續(xù)給藥過程中，分別于第26、52、78、104周四個(gè)時(shí)間點(diǎn)進(jìn)行系統(tǒng)解剖。所有實(shí)驗(yàn)動(dòng)物（包括實(shí)驗(yàn)過程中死亡的動(dòng)物）都進(jìn)行完整系統(tǒng)解剖及詳盡記錄觀察，其中肉眼可見的所有病變或可疑病變組織都進(jìn)行組織學(xué)水平的檢查。HE染色后，光學(xué)顯微鏡下觀察組織結(jié)構(gòu)的病理變化。 慢性毒性試驗(yàn)組織學(xué)檢查結(jié)果顯示，從第52周開始，對(duì)照組及不同給藥組的大鼠臟器中均發(fā)生不同性質(zhì)、不同程度的病理組織學(xué)改變，但這種改變屬于老齡化自發(fā)性的病變。到第104周，高劑量組中雌性大鼠腎小管上皮細(xì)胞的變性發(fā)生數(shù)與對(duì)照組相比存在極顯著的升高（P0.01），中劑量組雌性大鼠腎臟間質(zhì)炎的發(fā)生數(shù)也明顯高于對(duì)照組，這種變化也是顯著的（P0.05），而低劑量組則沒有顯著差異（P0.05）；而在雄性大鼠的腎組織中并沒有發(fā)生類似的變化。 致癌性組織學(xué)結(jié)果顯示，各組大鼠腫瘤絕大部分發(fā)生于78周以后。高、中、低劑量組與對(duì)照組之間，大鼠腫瘤發(fā)生率、潛伏期、多發(fā)性以及各類型腫瘤均未見顯著性差異，均是大鼠自發(fā)性病變。 上述結(jié)果說明，吡草醚原藥對(duì)SD大鼠沒有明顯的致癌作用，但是會(huì)導(dǎo)致腎臟發(fā)生明顯的器質(zhì)性病變，且該變化具有性別差異。本論文的發(fā)現(xiàn)對(duì)于進(jìn)一步的研究及生產(chǎn)使用具有一定程度的指導(dǎo)意義。
[Abstract]:Pyrazole is a kind of herbicide that can specifically inhibit protoporphyrin IX oxidase. It has good selectivity between animals and plants. Pyrazole is an optical herbicide. After application of foliage, it can quickly induce plant leaves to be necrotic or dry under the effect of light. So far, pyridosethers are the most active in the wheat field herbicides. It is necessary to systematically study the safety of drug use in a wide variety of varieties. In this paper, the chronic toxicity and carcinogenic effect of pyridaether on rats is discussed from the histopathological point of view.
640 rats were divided into 4 groups according to the chronic toxicity and carcinogenicity test of the GB15670-1995< pesticide registration test. The rats were randomly divided into 4 groups according to the animal weight, 1 blank control groups and 3 low, middle and high dose groups, each group was 160, and the female and male were half. In the course of continuous administration, the system was carried out on the system of Thursday at the time of 26,52,78104, respectively. Anatomy. All the experimental animals (including the dead animals in the process of the experiment) carried out complete systematic anatomy and detailed observation, in which all the lesions or suspicious lesions visible to the naked eye were examined by.HE staining, and the pathological changes of the tissue structure were observed under the optical microscope.
The histological examination of the chronic toxicity test showed that from the fifty-second weeks, there were different properties and different degrees of histopathological changes in the viscera of the control group and the different drug groups, but this change belonged to the spontaneous aging disease. By the 104th week, the number of the degeneration of the tubular epithelial cells in the high dose group of the female rats and the number of the degeneration of the renal tubular epithelial cells in the high dose group were the number and the number of the degeneration of the renal tubular epithelial cells in the high dose group. There was a very significant increase in the control group (P0.01), and the number of renal interstitial inflammation in the middle dose group was significantly higher than that in the control group (P0.05), but there was no significant difference in the low dose group (P0.05), but there was no similar change in the renal tissue of the male rats.
The results of carcinogenicity showed that most of the tumor occurred after 78 weeks. In the high, middle, low dose group and the control group, there was no significant difference in the incidence of tumor, latent period, multiple and various types of tumor, all of which were spontaneous lesions of rats.
These results suggest that pyridaether has no obvious carcinogenic effect on SD rats, but it will lead to obvious organic pathological changes in the kidney, and the changes have sex differences. The findings of this paper have some guidance for further research and production.
【學(xué)位授予單位】：中南民族大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R114
本文編號(hào)：2174764
[Abstract]:Pyrazole is a kind of herbicide that can specifically inhibit protoporphyrin IX oxidase. It has good selectivity between animals and plants. Pyrazole is an optical herbicide. After application of foliage, it can quickly induce plant leaves to be necrotic or dry under the effect of light. So far, pyridosethers are the most active in the wheat field herbicides. It is necessary to systematically study the safety of drug use in a wide variety of varieties. In this paper, the chronic toxicity and carcinogenic effect of pyridaether on rats is discussed from the histopathological point of view.
640 rats were divided into 4 groups according to the chronic toxicity and carcinogenicity test of the GB15670-1995< pesticide registration test. The rats were randomly divided into 4 groups according to the animal weight, 1 blank control groups and 3 low, middle and high dose groups, each group was 160, and the female and male were half. In the course of continuous administration, the system was carried out on the system of Thursday at the time of 26,52,78104, respectively. Anatomy. All the experimental animals (including the dead animals in the process of the experiment) carried out complete systematic anatomy and detailed observation, in which all the lesions or suspicious lesions visible to the naked eye were examined by.HE staining, and the pathological changes of the tissue structure were observed under the optical microscope.
The histological examination of the chronic toxicity test showed that from the fifty-second weeks, there were different properties and different degrees of histopathological changes in the viscera of the control group and the different drug groups, but this change belonged to the spontaneous aging disease. By the 104th week, the number of the degeneration of the tubular epithelial cells in the high dose group of the female rats and the number of the degeneration of the renal tubular epithelial cells in the high dose group were the number and the number of the degeneration of the renal tubular epithelial cells in the high dose group. There was a very significant increase in the control group (P0.01), and the number of renal interstitial inflammation in the middle dose group was significantly higher than that in the control group (P0.05), but there was no significant difference in the low dose group (P0.05), but there was no similar change in the renal tissue of the male rats.
The results of carcinogenicity showed that most of the tumor occurred after 78 weeks. In the high, middle, low dose group and the control group, there was no significant difference in the incidence of tumor, latent period, multiple and various types of tumor, all of which were spontaneous lesions of rats.
These results suggest that pyridaether has no obvious carcinogenic effect on SD rats, but it will lead to obvious organic pathological changes in the kidney, and the changes have sex differences. The findings of this paper have some guidance for further research and production.
【學(xué)位授予單位】：中南民族大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R114
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