鎘對河南華溪蟹血淋巴細胞的毒性效應(yīng)及脂多糖的調(diào)節(jié)作用
發(fā)布時間：2018-08-06 17:52
【摘要】：本博士學(xué)位論文以河南華溪蟹(Sinopotamon henanense)的血淋巴細胞(hemocytes)為材料,研究了亞慢性鎘染毒對血淋巴細胞的毒性作用及免疫增強劑脂多糖的調(diào)節(jié)作用。實驗設(shè)置了3個鎘處理組(0.725、1.450和2.900 mg/L)和一個空白對照組,分別處理7 d、14 d和21 d。染毒后將蟹體平均分為兩組,其中一組個體用來研究鎘的毒性作用,另一組個體分別注射10μg/mL濃度的脂多糖用來研究脂多糖對鎘毒性的調(diào)節(jié)作用。首先,采用透射電鏡技術(shù)(transmission electron microscope,TEM)觀察了血淋巴細胞的形態(tài)結(jié)構(gòu)變化及膜的完整性,從形態(tài)學(xué)角度探究鎘對血淋巴細胞超微結(jié)構(gòu)的損傷。其次,用血球計數(shù)板統(tǒng)計了血淋巴細胞的總數(shù)(total hemocyte counts,THC);采用火焰原子吸收儀檢測了血淋巴細胞中鎘的富集量;用比色法測定了活性氧自由基(reactive oxygen species,ROS)水平,酸性磷酸酶(acid phosphatase,ACP)、堿性磷酸酶(alkaline phosphatase,AKP)和溶菌酶(lysozyme,LSZ)等溶酶體酶的活性;利用中性紅染色法測定了溶酶體膜的穩(wěn)定性,系統(tǒng)研究了由于鎘的富集引起ROS水平升高而進一步介導(dǎo)的血淋巴細胞生理生化功能的變化,以及脂多糖對這種變化的調(diào)節(jié)作用。在此基礎(chǔ)上,為了研究抗氧化酶活性和氧化損傷程度,用購置的生化試劑盒測定了總抗氧化能力(total antioxidant capacity,T-AOC)、抗氧化酶:超氧化物歧化酶(superoxide dismutase,SOD)、過氧化氫酶(catalase,CAT)、谷胱甘肽過氧化物酶(glutathione peroxidase,GPx)和過氧化物酶(peroxidase,POD)活性的變化及脂質(zhì)過氧化產(chǎn)物丙二醛(malondialdehyde,MDA)的含量。此外,采用2,4-二硝基苯肼(DNPH)比色法測定了蛋白質(zhì)羰基(protein carbonyl derivates,PCO)含量,用KCL-SDS沉淀法檢測了DNA-蛋白質(zhì)交聯(lián)率(DNA-protein crosslink,DPC)。最后,利用實時熒光定量PCR法對免疫相關(guān)基因酚氧化酶原(prophenoloxidase,簡稱proPO)、溶菌酶(lysozyme,簡稱LSZ)和金屬硫蛋白(metallothionein,簡稱MT)三種基因mRNA表達水平的變化,用比色法測定了PO的活性,從分子水平上研究了鎘脅迫的應(yīng)答機制及脂多糖的調(diào)節(jié)作用,闡述了鎘對河南華溪蟹免疫系統(tǒng)的毒性作用及致毒機制。研究結(jié)果包括以下四個方面:1.鎘對河南華溪蟹血淋巴細胞超微結(jié)構(gòu)的影響根據(jù)細胞的整體形態(tài)、胞質(zhì)中的顆粒數(shù)目及其大小可將河南華溪蟹血淋巴細胞分為三類:大顆粒細胞、小顆粒細胞和無顆粒細胞。其中大顆粒細胞的體積較大、細胞核較小、胞質(zhì)中有較多的電子密度顆粒,而且各類細胞器較多;小顆粒細胞體積稍小,胞質(zhì)中含少量顆粒;無顆粒細胞體積一般較小,幾乎無顆粒,較少觀察到細胞器。鎘暴露可以引起溪蟹顆粒細胞進行脫顆粒,細胞核變形、染色質(zhì)濃縮,線粒體出現(xiàn)了嵴斷裂、空泡化甚至消失的現(xiàn)象,粗面內(nèi)質(zhì)網(wǎng)的腔擴張、其上的核糖體脫落,溶酶體膜模糊甚至破裂。另外還發(fā)現(xiàn),高濃度組(2.900 mg/L)鎘暴露時間由7 d延長至21 d時,血淋巴細胞超微結(jié)構(gòu)的損傷程度較低濃度組(0.725 mg/L)的嚴重。2.鎘處理對河南華溪蟹血淋巴細胞免疫相關(guān)因子的影響及脂多糖的調(diào)節(jié)作用河南華溪蟹血淋巴細胞鎘的富集量隨著染毒濃度的增加和暴露時間的延長,呈現(xiàn)上升趨勢,并且有劑量-效應(yīng)。鎘導(dǎo)致溪蟹血淋巴細胞總數(shù)顯著下降,ROS水平顯著上升,溶酶體膜的穩(wěn)定性下降。除LSZ外,鎘暴露引起了血淋巴細胞中的ACP和AKP活性均升高;鎘染毒后再注射脂多糖,與鎘單獨作用相比,脂多糖引起溪蟹血淋巴細胞總數(shù)下降,ROS在一定程度上降低,溶酶體膜的穩(wěn)定性也下降,ACP和AKP活力均升高,LSZ活力無顯著變化。3.鎘脅迫對河南華溪蟹血淋巴細胞抗氧化酶和氧化損傷的影響及脂多糖的調(diào)節(jié)作用河南華溪蟹血淋巴細胞中抗氧化酶SOD、CAT、GPx和POD的活性隨著鎘濃度的增加和處理時間的延長而整體上呈現(xiàn)出“先升高后降低”的趨勢,即低濃度鎘(0.725 mg/L)顯著誘導(dǎo)抗氧化酶活性升高,高濃度鎘(2.900 mg/L)抑制了酶活性。脂多糖作用后,四種酶活性在不同程度上均低于其相應(yīng)的鎘處理組的活性。血淋巴細胞中的氧化損傷指標:PCO、DPC和MDA含量均呈現(xiàn)逐漸升高趨勢。與鎘單獨作用相比,鎘暴露后再注射脂多糖,三個氧化損傷指標出現(xiàn)不同程度的下降,然而鎘暴露時間延長至21 d時,各氧化損傷指標無顯著下降趨勢,脂多糖在一定程度上減輕鎘導(dǎo)致的氧化損傷效應(yīng)。4.河南華溪蟹血淋巴細胞對鎘的免疫應(yīng)答及脂多糖的調(diào)節(jié)作用隨著鎘濃度的增加或者暴露時間的延長,河南華溪蟹血淋巴細胞中proPO mRNA的表達量在整體上呈現(xiàn)下降趨勢,與鎘單獨作用相比,脂多糖作用后,proPO mRNA的表達量升高。PO活性在整個處理過程中呈現(xiàn)“先升后降”趨勢。鎘暴露后注射脂多糖,與相應(yīng)的鎘單獨處理組相比,PO活性在整體上呈現(xiàn)下降趨勢。鎘誘導(dǎo)溪蟹血淋巴細胞中的LSZ mRNA表達水平均升高,并呈現(xiàn)出劑量-效應(yīng)。鎘暴露后注射脂多糖,與相應(yīng)的鎘單獨處理組相比,LSZ mRNA表達水平出現(xiàn)不同程度的下降趨勢。此外,隨著暴露時間的延長或者鎘濃度的增加,MT mRNA的表達水平有升高趨勢,并呈現(xiàn)時間和劑量-效應(yīng)。鎘暴露后再注射脂多糖時,與相應(yīng)的鎘作用組相比,MT mRNA的表達水平呈下降趨勢。研究結(jié)果表明:1.鎘脅迫改變了河南華溪蟹血淋巴細胞的超微結(jié)構(gòu),其中高濃度鎘暴露對血淋巴細胞造成的損傷更嚴重,說明血淋巴細胞是鎘毒性作用的靶器官,這些結(jié)構(gòu)上的損傷可能會影響免疫相關(guān)因子正常的合成和分泌,導(dǎo)致溪蟹失去正常的免疫功能。2.由于鎘在河南華溪蟹血淋巴細胞的富集而引起了ROS水平的升高,而ROS進一步對溶酶體造成損壞,釋放溶酶體酶;而脂多糖會降低ROS水平,在一定程度上能增強蟹體的免疫能力。3.鎘可以通過改變河南華溪蟹血淋巴細胞抗氧化酶活性,進而誘導(dǎo)脂質(zhì)、蛋白質(zhì)和DNA發(fā)生氧化損傷,最終使血淋巴細胞免疫功能下降。脂多糖可以調(diào)節(jié)抗氧化酶活性,使氧化損傷指標降低。4.鎘對河南華溪蟹血淋巴細胞免疫相關(guān)基因有明顯的影響,鎘抑制了proPO mRNA的表達,卻能誘導(dǎo)LSZ和MT兩種基因mRNA的表達,而脂多糖對這三種基因表達的調(diào)節(jié)作用不同。
[Abstract]:In this doctoral dissertation, the toxic effects of subchronic cadmium exposure on blood lymphocytes and the regulation of lipopolysaccharide were studied with the blood lymphocyte (hemocytes) of Sinopotamon henanense in Henan. The experiment set up 3 cadmium treatment groups (0.725,1.450 and 2.900 mg/L) and a blank control group, respectively, to deal with 7 D, 14 d and 21 D. were divided into two groups, one of which was used to study the toxicity of cadmium. The other group was injected with 10 g/mL concentration of lipopolysaccharide to study the regulation of lipopolysaccharide on cadmium toxicity. First, the blood lymphocytes were observed by transmission electron microscopy (transmission electron microscope, TEM). Morphological changes and membrane integrity were used to investigate the damage of cadmium to the ultrastructure of blood lymphocytes from the morphological angle. Secondly, the total number of blood lymphocytes (total hemocyte counts, THC) was measured by the blood cell count board, and the concentration of cadmium in blood lymphocytes was detected by flame atomic absorptiometer, and the reactive oxygen free radicals were measured by colorimetric method. The activity of lysosomal enzymes (reactive oxygen species, ROS), acid phosphatase (acid phosphatase, ACP), alkaline phosphatase (alkaline phosphatase, AKP), and lysozyme (lysozyme, LSZ)). The stability of the lysosomal membrane was determined by neutral red staining. The system was further mediated by the enrichment of cadmium. Changes in the physiological and biochemical functions of blood lymphocytes and the regulation of lipopolysaccharide on this change. On this basis, in order to study the activity of antioxidant enzymes and the degree of oxidative damage, the total antioxidant capacity (total antioxidant capacity, T-AOC), antioxidant enzyme: superoxide dismutase (superoxide dismuta) were measured by the purchased biochemical kit. Se, SOD), the changes in the activity of catalase (catalase, CAT), glutathione peroxidase (glutathione peroxidase, GPx) and peroxidase (peroxidase, POD), and the content of malondialdehyde (malondialdehyde, MDA) in the product of lipid peroxidation. Vates, PCO) content, the DNA- protein crosslinking rate (DNA-protein crosslink, DPC) was detected by KCL-SDS precipitation method. Finally, three kinds of genes were expressed by the real-time fluorescent quantitative PCR method for the immunologic related genes of phenoloxidase (prophenoloxidase, proPO), and the lysozyme (lysozyme, LSZ) and metallothionein. The activity of PO was measured by colorimetric method. The response mechanism of cadmium stress and the regulation of lipopolysaccharide were studied at the molecular level, and the toxic and toxic mechanisms of cadmium on the immune system of Henan crabs were expounded. The results included the following four aspects: 1. the influence of cadmium on the ultrastructure of the blood lymphocyte of Creek crabs According to the overall morphology of the cell, the number and size of the particles in the cytoplasm can be divided into three types: large granulosa cells, small granular cells and no granulosa cells in Henan. Large granular cells have larger size, smaller nuclei, more electron density particles in the cytoplasm, and many kinds of organelles; small granular cell bodies. The volume of the cytoplasm is small and the cytoplasm contains a small amount of particles; the volume of the granulosa cells is generally small and almost no particles. The cytoplasm is less observed. Cadmium exposure can cause degranulation, nuclear deformation, chromatin concentration, mitochondrial crista fracture, vacuolization to the disappearance, the dilation of the rough endoplasmic reticulum, and ribose on the cytoplasm. It was also found that the cadmium exposure time of the high concentration group (2.900 mg/L) was prolonged from 7 d to 21 d, and the damage degree of the ultrastructure of the blood lymphocyte was more than that of the low concentration group (0.725 mg/L), the effect of.2. treatment on the blood lymphatic cell immunity related factors of Henan Chinese crabs and the regulation of lipopolysaccharide The concentration of cadmium in the blood lymphocytes of crabs of South China brook increased with the increase of exposure concentration and exposure time, which showed an upward trend and had a dose effect. Cadmium led to a significant decrease in the total number of blood lymphocytes in crabs, the ROS level increased significantly, and the stability of the lysosome membrane decreased. In addition to LSZ, cadmium exposure caused ACP and AKP activities in blood lymphocytes. Compared with cadmium alone, lipopolysaccharide caused the decrease of the total number of blood lymphocytes in crabs, ROS decreased to a certain extent, the stability of the lysosome membrane decreased, the activity of ACP and AKP increased, and the activity of LSZ had no significant changes in.3. stress on the antioxidant enzymes and oxidative damage of the blood lymphocytes of Henan Chinese crabs. The effects of injury and the regulation of lipopolysaccharide on the activity of antioxidant enzymes SOD, CAT, GPx and POD in the blood lymphocytes of Henan Chinese crabs with the increase of cadmium concentration and the prolongation of treatment time, the trend of "first increase and then decrease" was presented as a whole, that is, low concentration of cadmium (0.725 mg/L) significantly induced the increase of antioxidant enzyme activity and high concentration of cadmium (2.900 mg/). L) inhibited the activity of the enzyme. After lipopolysaccharide, the activity of the four enzymes was lower than the activity of the corresponding cadmium treatment group. The oxidative damage indexes in the blood lymphocytes, such as PCO, DPC and MDA, were all increasing gradually. Compared with the cadmium alone, the cadmium was reinjected into lipopolysaccharide after exposure to cadmium, and the three oxidative damage indexes appeared in different processes. When the exposure time of cadmium was prolonged to 21 d, there was no significant decrease in the oxidative damage index, and the lipopolysaccharide alleviated the oxidative damage effect caused by cadmium to a certain extent,.4. Henan Chinese crab blood lymphocytes to the immune response to cadmium and the regulation of lipopolysaccharide with the increase of cadmium concentration or prolongation of exposure time, Henan Hua Hua. The expression of proPO mRNA in the blood lymphocytes of crabs showed a downward trend on the whole. Compared with the effect of cadmium alone, the expression of proPO mRNA increased after the action of lipopolysaccharide, and the.PO activity showed a "first rise and then descend" trend during the whole process. After cadmium exposure, the activity of PO was compared with that of the isolated treatment group. The level of LSZ mRNA expression in the blood lymphocytes of crabs induced by cadmium increased and showed a dose effect. After exposure to cadmium exposure to lipopolysaccharide, the expression level of LSZ mRNA decreased in different degrees. In addition, with the prolongation of exposure time or the increase of cadmium concentration, MT mRNA The expression level of MT mRNA was decreased when cadmium exposed to lipopolysaccharide after exposure to cadmium. The results showed that 1. cadmium stress changed the ultrastructure of the blood lymphocytes of Henan Chinese crabs, which caused by high concentration of cadmium exposure to blood lymphocytes. The damage is more serious, indicating that the blood lymphocyte is the target organ of the toxic effect of cadmium, the damage of these structures may affect the normal synthesis and secretion of immune related factors, which leads to the loss of normal immune function of the crabs,.2. increases the level of ROS because of the enrichment of cadmium in the blood lymphocytes of Henan crabs, and the ROS is further to the lysase. The body causes damage and release lysosomal enzyme, while lipopolysaccharide can reduce the ROS level and enhance the immune ability of the crab body to a certain extent,.3. cadmium can induce lipid, protein and DNA oxidative damage by changing the antioxidant enzyme activity of the blood lymphocytes of Henan crabs, and the immune function of blood lymphocytes can be reduced at the end of the year. The effects of.4. cadmium on the immune related genes in the blood lymphocytes of Henan Chinese crabs were obviously affected by the regulation of antioxidant enzyme activity. Cadmium inhibited the expression of proPO mRNA, but could induce the expression of LSZ and MT two genes mRNA, and the regulation of lipopolysaccharide on the expression of the three genes was different.
【學(xué)位授予單位】：山西大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2017
【分類號】：R114
本文編號：2168518
[Abstract]:In this doctoral dissertation, the toxic effects of subchronic cadmium exposure on blood lymphocytes and the regulation of lipopolysaccharide were studied with the blood lymphocyte (hemocytes) of Sinopotamon henanense in Henan. The experiment set up 3 cadmium treatment groups (0.725,1.450 and 2.900 mg/L) and a blank control group, respectively, to deal with 7 D, 14 d and 21 D. were divided into two groups, one of which was used to study the toxicity of cadmium. The other group was injected with 10 g/mL concentration of lipopolysaccharide to study the regulation of lipopolysaccharide on cadmium toxicity. First, the blood lymphocytes were observed by transmission electron microscopy (transmission electron microscope, TEM). Morphological changes and membrane integrity were used to investigate the damage of cadmium to the ultrastructure of blood lymphocytes from the morphological angle. Secondly, the total number of blood lymphocytes (total hemocyte counts, THC) was measured by the blood cell count board, and the concentration of cadmium in blood lymphocytes was detected by flame atomic absorptiometer, and the reactive oxygen free radicals were measured by colorimetric method. The activity of lysosomal enzymes (reactive oxygen species, ROS), acid phosphatase (acid phosphatase, ACP), alkaline phosphatase (alkaline phosphatase, AKP), and lysozyme (lysozyme, LSZ)). The stability of the lysosomal membrane was determined by neutral red staining. The system was further mediated by the enrichment of cadmium. Changes in the physiological and biochemical functions of blood lymphocytes and the regulation of lipopolysaccharide on this change. On this basis, in order to study the activity of antioxidant enzymes and the degree of oxidative damage, the total antioxidant capacity (total antioxidant capacity, T-AOC), antioxidant enzyme: superoxide dismutase (superoxide dismuta) were measured by the purchased biochemical kit. Se, SOD), the changes in the activity of catalase (catalase, CAT), glutathione peroxidase (glutathione peroxidase, GPx) and peroxidase (peroxidase, POD), and the content of malondialdehyde (malondialdehyde, MDA) in the product of lipid peroxidation. Vates, PCO) content, the DNA- protein crosslinking rate (DNA-protein crosslink, DPC) was detected by KCL-SDS precipitation method. Finally, three kinds of genes were expressed by the real-time fluorescent quantitative PCR method for the immunologic related genes of phenoloxidase (prophenoloxidase, proPO), and the lysozyme (lysozyme, LSZ) and metallothionein. The activity of PO was measured by colorimetric method. The response mechanism of cadmium stress and the regulation of lipopolysaccharide were studied at the molecular level, and the toxic and toxic mechanisms of cadmium on the immune system of Henan crabs were expounded. The results included the following four aspects: 1. the influence of cadmium on the ultrastructure of the blood lymphocyte of Creek crabs According to the overall morphology of the cell, the number and size of the particles in the cytoplasm can be divided into three types: large granulosa cells, small granular cells and no granulosa cells in Henan. Large granular cells have larger size, smaller nuclei, more electron density particles in the cytoplasm, and many kinds of organelles; small granular cell bodies. The volume of the cytoplasm is small and the cytoplasm contains a small amount of particles; the volume of the granulosa cells is generally small and almost no particles. The cytoplasm is less observed. Cadmium exposure can cause degranulation, nuclear deformation, chromatin concentration, mitochondrial crista fracture, vacuolization to the disappearance, the dilation of the rough endoplasmic reticulum, and ribose on the cytoplasm. It was also found that the cadmium exposure time of the high concentration group (2.900 mg/L) was prolonged from 7 d to 21 d, and the damage degree of the ultrastructure of the blood lymphocyte was more than that of the low concentration group (0.725 mg/L), the effect of.2. treatment on the blood lymphatic cell immunity related factors of Henan Chinese crabs and the regulation of lipopolysaccharide The concentration of cadmium in the blood lymphocytes of crabs of South China brook increased with the increase of exposure concentration and exposure time, which showed an upward trend and had a dose effect. Cadmium led to a significant decrease in the total number of blood lymphocytes in crabs, the ROS level increased significantly, and the stability of the lysosome membrane decreased. In addition to LSZ, cadmium exposure caused ACP and AKP activities in blood lymphocytes. Compared with cadmium alone, lipopolysaccharide caused the decrease of the total number of blood lymphocytes in crabs, ROS decreased to a certain extent, the stability of the lysosome membrane decreased, the activity of ACP and AKP increased, and the activity of LSZ had no significant changes in.3. stress on the antioxidant enzymes and oxidative damage of the blood lymphocytes of Henan Chinese crabs. The effects of injury and the regulation of lipopolysaccharide on the activity of antioxidant enzymes SOD, CAT, GPx and POD in the blood lymphocytes of Henan Chinese crabs with the increase of cadmium concentration and the prolongation of treatment time, the trend of "first increase and then decrease" was presented as a whole, that is, low concentration of cadmium (0.725 mg/L) significantly induced the increase of antioxidant enzyme activity and high concentration of cadmium (2.900 mg/). L) inhibited the activity of the enzyme. After lipopolysaccharide, the activity of the four enzymes was lower than the activity of the corresponding cadmium treatment group. The oxidative damage indexes in the blood lymphocytes, such as PCO, DPC and MDA, were all increasing gradually. Compared with the cadmium alone, the cadmium was reinjected into lipopolysaccharide after exposure to cadmium, and the three oxidative damage indexes appeared in different processes. When the exposure time of cadmium was prolonged to 21 d, there was no significant decrease in the oxidative damage index, and the lipopolysaccharide alleviated the oxidative damage effect caused by cadmium to a certain extent,.4. Henan Chinese crab blood lymphocytes to the immune response to cadmium and the regulation of lipopolysaccharide with the increase of cadmium concentration or prolongation of exposure time, Henan Hua Hua. The expression of proPO mRNA in the blood lymphocytes of crabs showed a downward trend on the whole. Compared with the effect of cadmium alone, the expression of proPO mRNA increased after the action of lipopolysaccharide, and the.PO activity showed a "first rise and then descend" trend during the whole process. After cadmium exposure, the activity of PO was compared with that of the isolated treatment group. The level of LSZ mRNA expression in the blood lymphocytes of crabs induced by cadmium increased and showed a dose effect. After exposure to cadmium exposure to lipopolysaccharide, the expression level of LSZ mRNA decreased in different degrees. In addition, with the prolongation of exposure time or the increase of cadmium concentration, MT mRNA The expression level of MT mRNA was decreased when cadmium exposed to lipopolysaccharide after exposure to cadmium. The results showed that 1. cadmium stress changed the ultrastructure of the blood lymphocytes of Henan Chinese crabs, which caused by high concentration of cadmium exposure to blood lymphocytes. The damage is more serious, indicating that the blood lymphocyte is the target organ of the toxic effect of cadmium, the damage of these structures may affect the normal synthesis and secretion of immune related factors, which leads to the loss of normal immune function of the crabs,.2. increases the level of ROS because of the enrichment of cadmium in the blood lymphocytes of Henan crabs, and the ROS is further to the lysase. The body causes damage and release lysosomal enzyme, while lipopolysaccharide can reduce the ROS level and enhance the immune ability of the crab body to a certain extent,.3. cadmium can induce lipid, protein and DNA oxidative damage by changing the antioxidant enzyme activity of the blood lymphocytes of Henan crabs, and the immune function of blood lymphocytes can be reduced at the end of the year. The effects of.4. cadmium on the immune related genes in the blood lymphocytes of Henan Chinese crabs were obviously affected by the regulation of antioxidant enzyme activity. Cadmium inhibited the expression of proPO mRNA, but could induce the expression of LSZ and MT two genes mRNA, and the regulation of lipopolysaccharide on the expression of the three genes was different.
【學(xué)位授予單位】：山西大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2017
【分類號】：R114
【參考文獻】
相關(guān)期刊論文 前10條
1 周妍英;井維鑫;董峰;王蘭;;脂多糖對鎘致河南華溪蟹免疫因子毒性的調(diào)節(jié)作用[J];生物技術(shù)通訊;2016年06期
2 張藝森;王旭;秦魏婷;宋明明;孫炳偉;;脂多糖調(diào)控PI3K/Akt通路增強中性粒細胞吞噬功能[J];江蘇大學(xué)學(xué)報(醫(yī)學(xué)版);2016年05期
3 張娟;魏克強;趙婷;;Cu~(2+)脅迫對克氏原螯蝦(Procambarus clarkii)酚氧化酶原激活系統(tǒng)活性的影響[J];農(nóng)業(yè)環(huán)境科學(xué)學(xué)報;2016年05期
4 羅正明;賈雷坡;劉秀麗;賈艷青;;水環(huán)境鎘對水生動物毒性的研究進展[J];食品工業(yè)科技;2015年15期
5 彭少邦;蔡樂;李泗清;;土壤鎘污染修復(fù)方法及生物修復(fù)研究進展[J];環(huán)境與發(fā)展;2014年03期
6 杜麗娜;余若禎;王海燕;陸楙;劉征濤;;重金屬鎘污染及其毒性研究進展[J];環(huán)境與健康雜志;2013年02期
7 雷雯雯;徐團;王蘭;;鎘誘導(dǎo)長江華溪蟹(Sinopotamon yangtsekiense)心肌細胞凋亡的研究[J];海洋與湖沼;2012年02期
8 王麗麗;夏斌;陳碧鵑;李傳慧;唐學(xué)璽;;鎘脅迫對蝦夷扇貝抗氧化防御系統(tǒng)的影響[J];海洋環(huán)境科學(xué);2012年01期
9 冼健安;王安利;苗玉濤;伍娟;;流式細胞術(shù)檢測脂多糖對羅氏沼蝦血細胞活性和免疫功能的影響[J];海洋學(xué)報(中文版);2011年01期
10 馬寨璞;張繁霜;佟霽坤;;甲殼動物免疫系統(tǒng)概述及其圖論表示[J];安徽農(nóng)業(yè)科學(xué);2011年01期
相關(guān)博士學(xué)位論文 前10條
1 王婧;鎘誘發(fā)氧化應(yīng)激相關(guān)毒性效應(yīng)與機理的研究[D];山東大學(xué);2016年
2 楊健;鎘對河南華溪蟹脂肪積累與代謝的影響及毒性機制研究[D];山西大學(xué);2015年
3 吳昊;鎘對河南華溪蟹消化系統(tǒng)的損傷作用和毒性機制的研究[D];山西大學(xué);2014年
4 許森;L型凝集素和Toll、IMD信號通路關(guān)鍵因子在日本對蝦先天免疫中的功能研究[D];山東大學(xué);2014年
5 嚴芳;甲殼動物血藍蛋白免疫學(xué)活性及其作用機制研究[D];汕頭大學(xué);2011年
6 劉靜;苯并（α）芘對櫛孔扇貝血細胞免疫毒性機制的研究[D];中國海洋大學(xué);2009年
7 孫杰;中國明對蝦（Fenneropenaeus Chinensis）幾種免疫相關(guān)因子的分離純化、基因克隆及相互關(guān)系研究[D];中國科學(xué)院研究生院（海洋研究所）;2009年
8 高宏偉;中國明對蝦病原檢測新方法及酚氧化酶通路相關(guān)基因的研究[D];中國科學(xué)院研究生院（海洋研究所）;2008年
9 柏世軍;水環(huán)境鎘對羅非魚的毒性作用和機理探討[D];浙江大學(xué);2006年
10 邢婧;櫛孔扇貝（Chlamys Farreri）血細胞及其免疫特性的研究[D];中國海洋大學(xué);2002年
，本文編號：2168518
本文鏈接：http://sikaile.net/yixuelunwen/yufangyixuelunwen/2168518.html
最近更新
教材專著
