【摘要】：[目的]探討不同劑量和時間2,4-二異氰酸甲苯酯(2,4-TDI)氣體暴露對小鼠血清中特異性Ig G抗體水平的影響。[方法]將清潔級(SPF)BALB/c雌性小鼠64只,按照染毒時間7 d和14 d隨機分為兩部分,共8組,每組8只,每部分分別設(shè)對照組及低(0.036 mg/m3)、中(0.14 mg/m3)、高(0.36 mg/m3)濃度暴露組,采用TDI連續(xù)動式吸入染毒,每天染毒4 h,記錄體重,于末次染毒24 h采血分離血清,采用酶聯(lián)免疫法檢測特異性Ig G含量。[結(jié)果]暴露7 d時,3個暴露組小鼠血清特異性Ig G抗體含量(光密度值)分別為(0.14±0.008 4),(0.14±0.006 4)及(0.14±0.007 6),與對照組(0.14±0.009 3)相比,差異無統(tǒng)計學(xué)意義(P0.05);連續(xù)暴露14 d后小鼠血清中特異性Ig G抗體含量,各組間差異有統(tǒng)計學(xué)意義(F=11.41,P0.05),進一步兩兩比較分析,中、高暴露組血清中特異性Ig G抗體含量分別為(0.17±0.020 0)和(0.18±0.021 0),均高于對照組(0.14±0.004 6)(P0.05),高暴露組血清中特異性Ig G抗體含量高于低暴露組(0.15±0.012 0)(P0.05);中暴露組的暴露14 d小鼠血清中特異性Ig G抗體含量為(0.17±0.020 0),高于暴露7 d(0.14±0.006 4)(P0.05);高暴露組的暴露14 d小鼠血清中特異性Ig G抗體含量為(0.18±0.021 0),高于7 d(0.14±0.007 6)(P0.05)。[結(jié)論]TDI氣體連續(xù)暴露濃度及時間均影響小鼠血清中特異性的Ig G抗體水平的表達(dá)。
[Abstract]:[objective] to investigate the effect of 2o 4 diisocyanate gas exposure at different doses and time on the level of specific IgG antibody in serum of mice. [methods] Sixty-four clean grade (SPF) BALB/c female mice were randomly divided into 8 groups according to the exposure time of 7 days and 14 days. Each part was divided into control group and low (0.036 mg/m3), middle (0.14 mg/m3), high (0.36 mg/m3) exposure group. The serum was continuously inhaled by TDI for 4 hours daily, and the body weight was recorded. The serum samples were collected for 24 hours after the last exposure. The specific IgG content was detected by enzyme linked immunosorbent assay (Elisa). [results] after 7 days of exposure, the specific IgG antibody levels (optical density) in serum of the three exposure groups were (0.14 鹵0.008 4), (, 0.14 鹵0.006 4) and (0.14 鹵0.007 6), respectively, compared with those of the control group (0.14 鹵0.009 3). There was no significant difference (P0.05); after 14 days of continuous exposure, the content of specific IgG antibody in serum of mice was statistically significant (FG 11.41 P 0.05). The specific IgG antibody levels in the serum of the high exposure group were (0. 17 鹵0.020) and (0. 18 鹵0.021), respectively, which were higher than those of the control group (0. 14 鹵0.004. 6) (P0.05), the level of serum specific IgG antibody in the high exposure group was higher than that in the low exposure group (0. 15 鹵0.012) (P0.05), and that in the middle exposure group was 14 days after exposure (P0.05). The specific IgG antibody content was (0.17 鹵0.020), higher than that in the 7th day (0.14 鹵0.006 4) (P0.05), and the specific IgG antibody level in the serum of the mice exposed to high exposure for 14 days was (0.18 鹵0.021), higher than that in the 7th day (0.14 鹵0.007 6) (P0.05). [conclusion] the concentration and time of continuous exposure of TDI affected the expression of specific IgG antibody in serum of mice.
【作者單位】： 中山大學(xué)公共衛(wèi)生學(xué)院預(yù)防醫(yī)學(xué)系;上海市浦東新區(qū)陸家嘴社區(qū)衛(wèi)生服務(wù)中心;
【分類號】：R114

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