肝腸間氧化應(yīng)激的相關(guān)性及食品的調(diào)節(jié)作用
發(fā)布時(shí)間：2018-08-03 21:04
【摘要】：肝臟和腸道是生物體進(jìn)行食物代謝和吸收的重要器官。近30年來(lái),大量臨床數(shù)據(jù)和流行病學(xué)調(diào)查顯示肝臟疾病和腸道疾病二者存在強(qiáng)烈的相關(guān)性。盡管肝腸同時(shí)病變或先后病變的發(fā)生機(jī)制還未被闡明,但已有許多研究發(fā)現(xiàn)氧化應(yīng)激在肝腸疾病相關(guān)性上起關(guān)鍵作用。因此,本論文開展了系列研究,通過(guò)顯示肝腸間活性氧(ROS)分布的關(guān)聯(lián)性,解析肝腸間氧化應(yīng)激的相關(guān)性,并探討生物體攝入抗氧化食品對(duì)肝腸氧化應(yīng)激乃至整體氧化應(yīng)激系統(tǒng)的調(diào)節(jié)作用。首先,探討了肝腸間氧化應(yīng)激在構(gòu)造上的相關(guān)性。使用ROS探針二氯熒光素雙醋酸鹽(DCFH-DA,25mg/kg體重)尾靜脈注射SD大鼠,其整體熒光成像中膽總管和十二指腸顯現(xiàn)ROS的特征熒光；緊接著,對(duì)膽總管和十二指腸進(jìn)行組織切片,二者熒光成像顯示,ROS的特征熒光均集中分布在結(jié)締組織層上——膽總管的ROS特征熒光集中分布在外膜層上,十二指腸的ROS特征熒光集中分布在粘膜下層,即從膽總管至十二指腸的結(jié)締組織層形成了熒光通道,說(shuō)明了從膽總管外膜層這一結(jié)締組織層連通到十二指腸粘膜下層的結(jié)締組織層,形成了ROS集中分布的通道。由此,我們推測(cè)肝臟氧化應(yīng)激狀態(tài)有可能通過(guò)膽管發(fā)生傳導(dǎo)。為進(jìn)一步證實(shí)上述猜測(cè),實(shí)驗(yàn)通過(guò)對(duì)新西蘭兔肝臟左肝葉中部注射20μL過(guò)氧化氫(30%,w/v)的方式誘導(dǎo)肝臟氧化應(yīng)激,監(jiān)測(cè)該氧化應(yīng)激所致流經(jīng)膽總管的電流變化。結(jié)果發(fā)現(xiàn),注射引起流經(jīng)膽總管的電流迅速上升,電子走向?yàn)閺母闻K至十二指腸,且電流的上升趨勢(shì)在心臟停止跳動(dòng)后依然持續(xù)一段時(shí)間,該電流有可能是線粒體在氧化應(yīng)激時(shí)呼吸鏈漏出的電子的遷移所形成的,說(shuō)明肝臟發(fā)生的氧化應(yīng)激可能以電子傳遞的方式通過(guò)膽管傳導(dǎo)至十二指腸壁。上述結(jié)果,為肝腸間氧化應(yīng)激的相關(guān)性提供了新的證據(jù)。為進(jìn)一步證實(shí)上述肝腸間氧化應(yīng)激相關(guān)性的結(jié)果,我們將專一性清除超氧陰離子自由基(O2.-)的跨膜蛋白酶(Tat-SOD融合蛋白)作用于腸道,分析腸道的抗氧化干預(yù)對(duì)肝臟氧化應(yīng)激狀態(tài)的調(diào)節(jié)作用。實(shí)驗(yàn)以腹腔注射四氯化碳(CCl4) (1mL/kg體重,每周兩次,持續(xù)6周給藥)誘導(dǎo)肝纖維化的SD大鼠為模型,Tat-SOD融合蛋白(25000U/kg體重,每天兩次,持續(xù)4周給藥)以經(jīng)口灌服方式進(jìn)行抗氧化干預(yù)治療。結(jié)果發(fā)現(xiàn),腸道上Tat-SOD融合蛋白的抗氧化干預(yù)均可顯著降低SD大鼠的血清谷丙轉(zhuǎn)氨酶(ALT)和谷草轉(zhuǎn)氨酶(AST)以及肝臟組織中丙二醛(MDA)含量至正常水平(P0.01),顯著升高肝臟組織中超氧化物歧化酶(SOD)、過(guò)氧化氫酶(CAT)和谷胱甘肽過(guò)氧化物酶(GSH-Px)活力(P0.01),并使呈彌漫性纖維化的肝臟得以逆轉(zhuǎn)再生,肝臟組織結(jié)構(gòu)得以顯著修復(fù)。上述結(jié)果說(shuō)明,經(jīng)口灌服Tat-SOD融合蛋白的抗氧化干預(yù)可以達(dá)到逆轉(zhuǎn)肝纖維化的治療作用。我們推測(cè)該Tat-SOD融合蛋白可能透皮進(jìn)入腸壁疏松結(jié)締組織清除該部位所富集或傳遞的02-,進(jìn)而可能通過(guò)膽管的橋連緩解肝臟的氧化應(yīng)激狀態(tài),從而起到對(duì)肝纖維化的修復(fù)。由于氧化應(yīng)激狀態(tài)可能以電子傳遞方式在結(jié)締組織層傳導(dǎo),且人體經(jīng)絡(luò)系統(tǒng)在解剖學(xué)上與筋膜等結(jié)締組織系統(tǒng)重疊,經(jīng)絡(luò)系統(tǒng)有可能是與之相連臟器氧化應(yīng)激的傳導(dǎo)和調(diào)控系統(tǒng),因此經(jīng)線上兩個(gè)穴位間電位的測(cè)定有可能精確反應(yīng)沿經(jīng)線氧化應(yīng)激的傳遞,以及反應(yīng)與經(jīng)線相連臟器的氧化應(yīng)激狀態(tài)。由此我們使用穴位間電位實(shí)時(shí)監(jiān)測(cè)法,進(jìn)一步在人體上檢視結(jié)締組織與氧化應(yīng)激的關(guān)系。實(shí)驗(yàn)選取胃經(jīng)的下巨虛穴位(ST39)和內(nèi)庭穴位(ST44)為穴位體表電位監(jiān)測(cè)點(diǎn),足三里穴位(ST36)為穴位干預(yù)點(diǎn),分別給予足三里穴位單純按摩、0.2mL空白藥膏按摩、0.2mL Tat-SOD (3000U/mL)藥膏按摩、注射100μL還原型谷胱甘肽鈉(0.12g/mL)和針灸五種干預(yù),考察其對(duì)胃經(jīng)下游相鄰兩穴位(下巨虛與內(nèi)庭)間電位的影響。結(jié)果顯示：下巨虛與內(nèi)庭間電位隨足三里上按摩的施加或停止出現(xiàn)上升或回落；在按摩停止后抗氧化干預(yù)組的穴位間電位依然在高處波動(dòng),且在干預(yù)48小時(shí)后抗氧化干預(yù)組的穴位間電位顯著低于物理干預(yù)組,說(shuō)明穴位間電位可準(zhǔn)確反映經(jīng)線上氧化應(yīng)激的傳導(dǎo)。該監(jiān)測(cè)方法有可能成為實(shí)時(shí)監(jiān)測(cè)與經(jīng)線相連臟器的工作狀態(tài)。最后,我們采用穴位間電位實(shí)時(shí)監(jiān)測(cè)方法,跟蹤紅茶(金駿眉)、烏龍茶(水仙巖茶)和綠茶(高山綠茶)三種茶湯的攝入對(duì)人體手少陰心經(jīng)、手太陰肺經(jīng)、足少陰腎經(jīng)、足太陰脾經(jīng)、足厥陰肝經(jīng)、手太陽(yáng)小腸經(jīng)、足少陽(yáng)膽經(jīng)和足陽(yáng)明胃經(jīng)這八條經(jīng)絡(luò)的體表電位影響和即刻生理效應(yīng)。結(jié)果顯示,三種茶湯對(duì)手太陽(yáng)小腸經(jīng)的體表電位的影響最為顯著；三種茶湯均可產(chǎn)生即刻生理效應(yīng),可出現(xiàn)打嗝、生津、出汗、打哈欠、腸道蠕動(dòng)、手心生熱等生理變化,其中又以紅茶的即刻效應(yīng)最明顯,作用時(shí)間最長(zhǎng)；經(jīng)絡(luò)體表電位的波動(dòng)都有相應(yīng)的生理反應(yīng)。其次,我們還測(cè)定了三種茶湯的體外抗氧化活力和粒徑分布,以及跟蹤了動(dòng)物模型飲茶的長(zhǎng)期生理效應(yīng)(三種茶湯的攝入對(duì)以高脂飼料誘導(dǎo)的C57BL/6小鼠肝腸組織氧化應(yīng)激等指標(biāo)的影響),并將其與茶湯的人體經(jīng)絡(luò)體表電位變化和即刻生理效應(yīng)進(jìn)行對(duì)比分析,結(jié)果表明,紅茶茶湯的粒徑最小最均一,其即刻生理效應(yīng)和引起的人體經(jīng)絡(luò)穴位間電位的變化也最明顯,可在一定程度上反應(yīng)茶湯的顆粒大小可影響其即刻效應(yīng)；紅茶茶湯的體外抗氧化活性和茶多酚含量最低,但其引起的經(jīng)絡(luò)體表電位變化卻最明顯,說(shuō)明了體外實(shí)驗(yàn)的抗氧化活力的分析結(jié)果,不一定能在體內(nèi)實(shí)驗(yàn)得到相應(yīng)的表現(xiàn)；三種茶湯均具有抑制肥胖形成和緩解肝腸間氧化應(yīng)激的作用。我們推測(cè),茶湯的抗氧化納米顆粒通過(guò)清除腸壁疏松結(jié)締組織層的ROS,引起腸道平滑肌縮收,產(chǎn)生打嗝等生理反應(yīng),并可能借助膽管的橋連進(jìn)一步解除肝臟的氧化應(yīng)激,從而預(yù)防脂肪肝及肥胖的發(fā)生；同時(shí)由于人體生物電網(wǎng)絡(luò)系統(tǒng)的存在,肝腸間氧化應(yīng)激的傳遞可能引發(fā)與各臟器相連經(jīng)線上的電位變化。以上研究結(jié)果,為肝腸間氧化應(yīng)激的相關(guān)性提供一個(gè)有力的證據(jù),初步說(shuō)明了腸道可能為肝臟ROS的新處理場(chǎng)；證實(shí)通過(guò)穴位間電位監(jiān)測(cè)與經(jīng)線相連器官氧化應(yīng)激狀態(tài)的可行性；揭示了食品除了提供營(yíng)養(yǎng)和能量外還具有調(diào)節(jié)人體氧化應(yīng)激處理體系的作用；為消化道、腸道疾病等提供嶄新的視角和方向,將對(duì)食品與人體相互作用的認(rèn)識(shí)提高到新的層次。
[Abstract]:The liver and intestines are important organs for the metabolism and absorption of food. In the past 30 years, a large number of clinical data and epidemiological investigations have shown that there is a strong correlation between the two groups of liver diseases and intestinal diseases. Although the pathogenesis of liver and intestinal lesions or successively pathological changes has not been explained, many studies have found that oxidative stress has been found. The correlation of liver and intestinal diseases plays a key role. Therefore, a series of studies have been carried out in this paper to analyze the correlation between the distribution of active oxygen (ROS) in the liver and intestine, to analyze the correlation between the oxidative stress in the liver and the intestine, and to explore the regulation of the antioxidative food on the oxidative stress of liver and the whole body and the whole body oxidation stress system. The correlation of the interintestinal oxidative stress on the structure. SD rats were injected with the ROS probe two chlorofluorescein diacetate (DCFH-DA, 25mg/kg weight) tail vein. The overall fluorescence imaging of the common bile duct and duodenum showed the characteristic fluorescence of ROS; then, the common bile duct and duodenum were sectioning, and the two fluorescence imaging showed that ROS was special. The ROS characteristic fluorescence of the choledochus is concentrated on the outer membrane layer. The ROS characteristic fluorescence of the duodenum is concentrated in the submucosa, that is, the fluorescent channel is formed from the connective tissue layer of the common bile duct to the duodenum, indicating that the connective tissue layer from the outer membrane layer of the choledochus is connected to ten. Two refers to the connective tissue layer of the submucosa of the intestinal mucosa, which forms a ROS concentrated channel. Thus, we speculate that the oxidative stress in the liver may be transmitted through the bile duct. To further confirm the above speculation, the experimental liver oxidative stress was induced by injecting 20 mu hydroperoxide (30%, w/v) into the central left hepatic lobe of New Zealand rabbit. The current changes in the common bile duct caused by the oxidative stress. It was found that the current caused by the injection of the common bile duct increased rapidly, the electron trend was from the liver to the duodenum, and the trend of the current continued to continue for a period after the heart stopped beating. The current could be the electricity that the mitochondria leaked out of the respiratory chain during oxidative stress. The migration of the children indicates that the oxidative stress in the liver may be transmitted through the bile duct to the duodenal wall electronically. The above results provide new evidence for the correlation of interintestinal oxidative stress. To further confirm the results of the above interhepatic oxidative stress correlation, we will specifically eliminate superoxide anion. The free radical (O2.-) transmembrane protease (Tat-SOD fusion protein) acts on the intestinal tract and analyzes the regulating effect of antioxidant intervention on the oxidative stress state of the liver. The experimental SD rats with SD (1mL/kg weight, two times a week, 6 weeks of administration) were used to induce liver fibrosis in SD rats, and the Tat-SOD fusion protein (25000U/kg body). The results showed that the antioxidant intervention of Tat-SOD fusion protein in the intestinal tract could significantly reduce the serum alanine transaminase (ALT) and glutamic aminotransferase (AST) and the content of malondialdehyde (MDA) in the liver tissue to normal level (P0.01) and significantly increase the liver of SD rats. In the dirty tissue, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activity (P0.01), and the diffuse fibrosis of the liver can be reversed and regenerated. The liver tissue structure can be restored significantly. The results show that the antioxidant intervention of the Tat-SOD fusion protein by oral administration can reverse the liver fiber. We speculate that the Tat-SOD fusion protein may be transdermal into the loose connective tissue of the intestinal wall to remove the 02- that is enriched or transmitted by the site, and thus may relieve the oxidative stress state of the liver through the bridge of the bile duct, thus repairing the liver fibrosis. The tissue layer is transmitted, and the human meridian system overlaps anatomically with the fascia and other connective tissue systems. The meridian system may be the conduction and regulation system of the oxidative stress associated with the organs. Therefore, the measurement of the potential of the two acupoints on the meridian may accurately reflect the transmission of the oxidative stress along the meridian, and the reaction and the meridian viscera. The relationship between the connective tissue and oxidative stress is further examined in the human body by the method of real-time monitoring of the acupoint potential. The test points for the surface potential of the acupoint (ST39) and the acupoint (ST44) of the stomach meridian are selected as the monitoring point of the acupoint body surface potential, and the Zusanli point (ST36) is the point intervention point of the acupoint, and the point single point is given to the Zusanli point single point, respectively. Pure massage, 0.2mL blank ointment massage, 0.2mL Tat-SOD (3000U/mL) ointment massage, injection of 100 micron L, glutathione sodium (0.12g/mL) and acupuncture and moxibustion, to investigate its effect on the potential of two acupoints (lower giant deficiency and inner atrium) adjacent to the lower reaches of the stomach meridian. The results show that the lower giant deficiency and the interting potential with the application or stop of the massage on the Zusanli. The acupoint potential of the anti oxidation intervention group still fluctuates at high level after the stop of the massage, and the point potential of the acupoint in the antioxidant intervention group is significantly lower than that in the physical intervention group after 48 hours of intervention, indicating that the acupoint potential can accurately reflect the conduction of oxidative stress on the meridian. The meridian line connects the working state of the viscera. Finally, we use the real-time monitoring method of the acupoint potential, tracking the three kinds of tea soup of black tea (Jin Jun eyebrow), oolong tea (Narcissus rock tea) and green tea (alpine green tea) to the human hand less Yin heart meridian, hand Taiyin lung meridian, foot less Yin kidney meridian, foot Taiyin splenic meridian, foot syncope Yin liver meridian, hand sun small intestine meridian foot, foot Shaoyang gallbladder The effect of body surface potential and immediate physiological effect on the eight meridians of the meridian and Foot Yangming Meridian showed that the body surface potential of the three kinds of tea soup was the most significant. The three kinds of tea soup could produce immediate physiological effects, and there were some physiological changes such as hiccup, birth, sweat, Kazakhstan, intestinal peristalsis, hand heart and other physiological changes. The immediate effect of black tea was most obvious, the action time was the longest, the fluctuation of the surface potential of the meridians and collaterals had corresponding physiological responses. Secondly, we also measured the antioxidant activity and particle size distribution of the three kinds of tea soup in vitro, and tracked the long-term physiological effects of the animal model drinking tea (three kinds of tea soup intake to the high fat diet induced C57BL/6 The effects of oxidative stress on the liver and intestinal tissue of mice were compared with the changes of the body surface potential and immediate physiological effects of the body and collaterals of the tea soup. The results showed that the smallest size of the black tea tea soup was the smallest, the immediate physiological effect and the changes of the potential of the meridian acupoints caused by the human body were the most obvious, and to a certain extent, it could be reversed to a certain extent. The particle size of the tea soup can affect its immediate effect; the antioxidant activity and the content of tea polyphenols in the tea tea soup in vitro are the lowest, but the changes in the surface potential of the meridians and collaterals are the most obvious. The results of the analysis of the antioxidant activity in the experiment in vitro may not have the corresponding performance in the experiment in the body; the three kinds of tea soup all have the inhibition. Obesity forms and alleviates the effect of interintestinal oxidative stress. We speculate that the antioxidant nanoparticles of the tea soup can reduce the oxidative stress of the liver by removing the ROS of the loose connective tissue layer of the intestinal wall, causing the contraction of the intestinal smooth muscle, producing hiccups, and preventing the fatty liver and obesity by using the bridge of the bile duct to further relieve the oxidative stress of the liver. At the same time, due to the existence of the human bioelectrical network system, the transmission of interintestinal oxidative stress may lead to potential changes on the lines connected to the organs. The above results provide a powerful evidence for the correlation of the hepatic interintestinal oxidative stress, which preliminarily indicates that the intestinal tract may be a new treatment field for the liver ROS; it is confirmed through the acupoint The feasibility of interposition potential monitoring and the oxidative stress state of the interconnected organs of the meridians revealed that food in addition to providing nutrition and energy also has the role of regulating the system of human oxidative stress treatment, providing new perspectives and directions for digestive tract and intestinal diseases, and raising the understanding of the interaction between food and human body to a new level.
【學(xué)位授予單位】：浙江工商大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2016
【分類號(hào)】：R151
本文編號(hào)：2162989
[Abstract]:The liver and intestines are important organs for the metabolism and absorption of food. In the past 30 years, a large number of clinical data and epidemiological investigations have shown that there is a strong correlation between the two groups of liver diseases and intestinal diseases. Although the pathogenesis of liver and intestinal lesions or successively pathological changes has not been explained, many studies have found that oxidative stress has been found. The correlation of liver and intestinal diseases plays a key role. Therefore, a series of studies have been carried out in this paper to analyze the correlation between the distribution of active oxygen (ROS) in the liver and intestine, to analyze the correlation between the oxidative stress in the liver and the intestine, and to explore the regulation of the antioxidative food on the oxidative stress of liver and the whole body and the whole body oxidation stress system. The correlation of the interintestinal oxidative stress on the structure. SD rats were injected with the ROS probe two chlorofluorescein diacetate (DCFH-DA, 25mg/kg weight) tail vein. The overall fluorescence imaging of the common bile duct and duodenum showed the characteristic fluorescence of ROS; then, the common bile duct and duodenum were sectioning, and the two fluorescence imaging showed that ROS was special. The ROS characteristic fluorescence of the choledochus is concentrated on the outer membrane layer. The ROS characteristic fluorescence of the duodenum is concentrated in the submucosa, that is, the fluorescent channel is formed from the connective tissue layer of the common bile duct to the duodenum, indicating that the connective tissue layer from the outer membrane layer of the choledochus is connected to ten. Two refers to the connective tissue layer of the submucosa of the intestinal mucosa, which forms a ROS concentrated channel. Thus, we speculate that the oxidative stress in the liver may be transmitted through the bile duct. To further confirm the above speculation, the experimental liver oxidative stress was induced by injecting 20 mu hydroperoxide (30%, w/v) into the central left hepatic lobe of New Zealand rabbit. The current changes in the common bile duct caused by the oxidative stress. It was found that the current caused by the injection of the common bile duct increased rapidly, the electron trend was from the liver to the duodenum, and the trend of the current continued to continue for a period after the heart stopped beating. The current could be the electricity that the mitochondria leaked out of the respiratory chain during oxidative stress. The migration of the children indicates that the oxidative stress in the liver may be transmitted through the bile duct to the duodenal wall electronically. The above results provide new evidence for the correlation of interintestinal oxidative stress. To further confirm the results of the above interhepatic oxidative stress correlation, we will specifically eliminate superoxide anion. The free radical (O2.-) transmembrane protease (Tat-SOD fusion protein) acts on the intestinal tract and analyzes the regulating effect of antioxidant intervention on the oxidative stress state of the liver. The experimental SD rats with SD (1mL/kg weight, two times a week, 6 weeks of administration) were used to induce liver fibrosis in SD rats, and the Tat-SOD fusion protein (25000U/kg body). The results showed that the antioxidant intervention of Tat-SOD fusion protein in the intestinal tract could significantly reduce the serum alanine transaminase (ALT) and glutamic aminotransferase (AST) and the content of malondialdehyde (MDA) in the liver tissue to normal level (P0.01) and significantly increase the liver of SD rats. In the dirty tissue, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activity (P0.01), and the diffuse fibrosis of the liver can be reversed and regenerated. The liver tissue structure can be restored significantly. The results show that the antioxidant intervention of the Tat-SOD fusion protein by oral administration can reverse the liver fiber. We speculate that the Tat-SOD fusion protein may be transdermal into the loose connective tissue of the intestinal wall to remove the 02- that is enriched or transmitted by the site, and thus may relieve the oxidative stress state of the liver through the bridge of the bile duct, thus repairing the liver fibrosis. The tissue layer is transmitted, and the human meridian system overlaps anatomically with the fascia and other connective tissue systems. The meridian system may be the conduction and regulation system of the oxidative stress associated with the organs. Therefore, the measurement of the potential of the two acupoints on the meridian may accurately reflect the transmission of the oxidative stress along the meridian, and the reaction and the meridian viscera. The relationship between the connective tissue and oxidative stress is further examined in the human body by the method of real-time monitoring of the acupoint potential. The test points for the surface potential of the acupoint (ST39) and the acupoint (ST44) of the stomach meridian are selected as the monitoring point of the acupoint body surface potential, and the Zusanli point (ST36) is the point intervention point of the acupoint, and the point single point is given to the Zusanli point single point, respectively. Pure massage, 0.2mL blank ointment massage, 0.2mL Tat-SOD (3000U/mL) ointment massage, injection of 100 micron L, glutathione sodium (0.12g/mL) and acupuncture and moxibustion, to investigate its effect on the potential of two acupoints (lower giant deficiency and inner atrium) adjacent to the lower reaches of the stomach meridian. The results show that the lower giant deficiency and the interting potential with the application or stop of the massage on the Zusanli. The acupoint potential of the anti oxidation intervention group still fluctuates at high level after the stop of the massage, and the point potential of the acupoint in the antioxidant intervention group is significantly lower than that in the physical intervention group after 48 hours of intervention, indicating that the acupoint potential can accurately reflect the conduction of oxidative stress on the meridian. The meridian line connects the working state of the viscera. Finally, we use the real-time monitoring method of the acupoint potential, tracking the three kinds of tea soup of black tea (Jin Jun eyebrow), oolong tea (Narcissus rock tea) and green tea (alpine green tea) to the human hand less Yin heart meridian, hand Taiyin lung meridian, foot less Yin kidney meridian, foot Taiyin splenic meridian, foot syncope Yin liver meridian, hand sun small intestine meridian foot, foot Shaoyang gallbladder The effect of body surface potential and immediate physiological effect on the eight meridians of the meridian and Foot Yangming Meridian showed that the body surface potential of the three kinds of tea soup was the most significant. The three kinds of tea soup could produce immediate physiological effects, and there were some physiological changes such as hiccup, birth, sweat, Kazakhstan, intestinal peristalsis, hand heart and other physiological changes. The immediate effect of black tea was most obvious, the action time was the longest, the fluctuation of the surface potential of the meridians and collaterals had corresponding physiological responses. Secondly, we also measured the antioxidant activity and particle size distribution of the three kinds of tea soup in vitro, and tracked the long-term physiological effects of the animal model drinking tea (three kinds of tea soup intake to the high fat diet induced C57BL/6 The effects of oxidative stress on the liver and intestinal tissue of mice were compared with the changes of the body surface potential and immediate physiological effects of the body and collaterals of the tea soup. The results showed that the smallest size of the black tea tea soup was the smallest, the immediate physiological effect and the changes of the potential of the meridian acupoints caused by the human body were the most obvious, and to a certain extent, it could be reversed to a certain extent. The particle size of the tea soup can affect its immediate effect; the antioxidant activity and the content of tea polyphenols in the tea tea soup in vitro are the lowest, but the changes in the surface potential of the meridians and collaterals are the most obvious. The results of the analysis of the antioxidant activity in the experiment in vitro may not have the corresponding performance in the experiment in the body; the three kinds of tea soup all have the inhibition. Obesity forms and alleviates the effect of interintestinal oxidative stress. We speculate that the antioxidant nanoparticles of the tea soup can reduce the oxidative stress of the liver by removing the ROS of the loose connective tissue layer of the intestinal wall, causing the contraction of the intestinal smooth muscle, producing hiccups, and preventing the fatty liver and obesity by using the bridge of the bile duct to further relieve the oxidative stress of the liver. At the same time, due to the existence of the human bioelectrical network system, the transmission of interintestinal oxidative stress may lead to potential changes on the lines connected to the organs. The above results provide a powerful evidence for the correlation of the hepatic interintestinal oxidative stress, which preliminarily indicates that the intestinal tract may be a new treatment field for the liver ROS; it is confirmed through the acupoint The feasibility of interposition potential monitoring and the oxidative stress state of the interconnected organs of the meridians revealed that food in addition to providing nutrition and energy also has the role of regulating the system of human oxidative stress treatment, providing new perspectives and directions for digestive tract and intestinal diseases, and raising the understanding of the interaction between food and human body to a new level.
【學(xué)位授予單位】：浙江工商大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2016
【分類號(hào)】：R151
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