本文選題：出生前暴露 + 1800MHz電磁波�。� 參考：《昆明醫(yī)科大學(xué)》2017年碩士論文

【摘要】：[目的]探測(cè)1800MHz電磁波和1800MHz與WiFi疊加射頻場(chǎng)暴露對(duì)仔代行為發(fā)育及認(rèn)知的影響。[方法]健康7周齡SPF級(jí)Wistar大鼠36只,雌鼠24只,雄鼠12只,適應(yīng)環(huán)境一周后,雌雄鼠2:1合籠交配后將孕鼠隨機(jī)分為兩個(gè)實(shí)驗(yàn)組和兩個(gè)對(duì)照組,每組6只。實(shí)驗(yàn)一組接受1800MHz電磁波(功率密度為1mW/cm2)暴露,實(shí)驗(yàn)二組接受1800MHz和WiFi疊加射頻場(chǎng)(功率密度為1mW/cm2)暴露,暴露時(shí)間從孕0天至孕20天于每晚20:00～次日清晨8:00,各實(shí)驗(yàn)組分別設(shè)置虛擬暴露作為對(duì)照。暴露期間動(dòng)物自由飲水和飲食,且環(huán)境背景(溫度、濕度、背景噪聲、通風(fēng)、光照等)條件穩(wěn)定。暴露完成后,待孕鼠產(chǎn)下仔鼠,觀查并測(cè)量生理發(fā)育和行為發(fā)育指標(biāo),待仔鼠長(zhǎng)至三周齡和七周齡測(cè)水迷宮后取海馬組織用高效液相色譜儀檢測(cè)四種氨基酸類(lèi)神經(jīng)遞質(zhì)(Glu、Gly、GABA、Asp)含量。[結(jié)果]1.發(fā)育指標(biāo):(1)生理發(fā)育指標(biāo):體重、睜眼時(shí)間兩實(shí)驗(yàn)組與對(duì)照組比較有差異(p0.05),與對(duì)照組比較體重存在下降趨勢(shì),睜眼時(shí)間延長(zhǎng);其他生理發(fā)育指標(biāo)均無(wú)統(tǒng)計(jì)學(xué)差異。(2)行為發(fā)育指標(biāo):前肢懸掛達(dá)標(biāo)日齡兩實(shí)驗(yàn)組與對(duì)照組比較有差異(p0.05),與對(duì)照組比較達(dá)標(biāo)日齡降低,其他行為發(fā)育指標(biāo)均無(wú)統(tǒng)計(jì)學(xué)差異。2.水迷宮實(shí)驗(yàn):(1)三周齡仔鼠水迷宮實(shí)驗(yàn):1800MHz電磁波暴露下,可視平臺(tái)實(shí)驗(yàn),雄性仔鼠實(shí)驗(yàn)第二天實(shí)驗(yàn)組較對(duì)照組逃避潛伏期延長(zhǎng)(p0.05),雌性仔鼠實(shí)驗(yàn)第一天和第二天實(shí)驗(yàn)組較對(duì)照組逃避潛伏期延長(zhǎng)(p0.05);隱匿平臺(tái)實(shí)驗(yàn),雄性仔鼠實(shí)驗(yàn)第一天至第四天實(shí)驗(yàn)組較對(duì)照組逃避潛伏期延長(zhǎng)(p0.05),雌性仔鼠實(shí)驗(yàn)第一天至第三天實(shí)驗(yàn)組較對(duì)照組逃避潛伏期延長(zhǎng)(p0.05)。1800MHz與WiFi疊加射頻場(chǎng)暴露下,可視平臺(tái)實(shí)驗(yàn),雌性仔鼠實(shí)驗(yàn)第一天實(shí)驗(yàn)組較對(duì)照組避潛伏期延長(zhǎng)(p0.05);隱匿平臺(tái)實(shí)驗(yàn),雄性和雌性仔鼠實(shí)驗(yàn)第一天至第四天實(shí)驗(yàn)組均較對(duì)照組逃避潛伏期延長(zhǎng)(p0.05)。(2)七周齡仔鼠水迷宮實(shí)驗(yàn):1800MHz電磁波暴露下,可視平臺(tái)實(shí)驗(yàn),雄性仔鼠實(shí)驗(yàn)第二天實(shí)驗(yàn)組較對(duì)照組逃避潛伏期延長(zhǎng)(p0.05);隱匿平臺(tái)實(shí)驗(yàn),雄性仔鼠實(shí)驗(yàn)第二天與第三天實(shí)驗(yàn)組較對(duì)照組逃避潛伏期延長(zhǎng)(p0.05),雌性仔鼠實(shí)驗(yàn)第一天實(shí)驗(yàn)組較對(duì)照組逃避潛伏期縮短(p0.05);空間探索實(shí)驗(yàn),雌性仔鼠穿臺(tái)次數(shù)實(shí)驗(yàn)組較對(duì)照組多(p0.05),平臺(tái)象限時(shí)間百分比(%)較對(duì)照組高(p0.05)。1800MHz與WiFi疊加射頻場(chǎng)暴露下,隱匿平臺(tái)實(shí)驗(yàn),雄性仔鼠實(shí)驗(yàn)第一天、第三天和第四天實(shí)驗(yàn)組均較對(duì)照組逃避潛伏期縮短(p0.05),雌性仔鼠實(shí)驗(yàn)第四天實(shí)驗(yàn)組較對(duì)照組逃避潛伏期縮短(p0.05);空間探索實(shí)驗(yàn),雄性仔鼠平臺(tái)象限時(shí)間百分比(%)實(shí)驗(yàn)組較對(duì)照組高(p0.05)。3.四種氨基酸類(lèi)神經(jīng)遞質(zhì)測(cè)定:1800MHz電磁波暴露下,實(shí)驗(yàn)組三周齡仔鼠海馬組織Asp表達(dá)量與對(duì)照組比較表達(dá)上調(diào)(p0.05)。1800MHz與WiFi疊加射頻場(chǎng)暴露下,實(shí)驗(yàn)組三周齡仔鼠海馬組織Asp表達(dá)量與對(duì)照組比較表達(dá)上調(diào)(p0.05),七周齡仔鼠海馬組織Glu表達(dá)量與對(duì)照比較表達(dá)下調(diào)(p0.05);三周齡與七周齡比較,實(shí)驗(yàn)組三周齡仔鼠海馬組織Asp表達(dá)量較七周齡高,(p0.05)。[結(jié)論]本實(shí)驗(yàn)條件下:1. 1800MHz電磁波和1800MHz與WiFi疊加射頻場(chǎng)暴露對(duì)仔鼠體重和睜眼時(shí)間有影響;對(duì)前肢懸掛影響還需進(jìn)一步驗(yàn)證。2. 1800MHz電磁波和1800MHz與WiFi疊加射頻場(chǎng)暴露對(duì)三周齡仔鼠空間學(xué)習(xí)能力有影響,未發(fā)現(xiàn)對(duì)三周齡仔鼠空間記憶能力的影響;對(duì)七周齡仔鼠空間學(xué)習(xí)與記憶能力影響還需進(jìn)一步驗(yàn)證。3. 1800MHz電磁波和1800MHz與WiFi疊加射頻場(chǎng)暴露使三周齡仔鼠海馬組織氨基酸類(lèi)神經(jīng)遞質(zhì)Asp表達(dá)上調(diào);后者可使七周齡仔鼠Glu表達(dá)下調(diào)。4.尚不明確WiFi對(duì)仔鼠學(xué)習(xí)記憶的影響。
[Abstract]:[Objective] to detect the effects of 1800MHz electromagnetic wave and 1800MHz and WiFi superposition radio frequency field exposure on the behavior development and cognition of the offspring. [Methods] 36 healthy 7 week old Wistar rats, 24 female rats and 12 male rats, after one week of adaptation to the environment, the female and male rats were randomly divided into two experimental groups and two control groups after 2:1 cage mating, and 6 rats in each group were tested. One group was exposed to 1800MHz electromagnetic wave (power density 1mW/cm2). The two groups were exposed to 1800MHz and WiFi superposition radiofrequency field (power density 1mW/cm2). The exposure time from 0 days of pregnancy to 20 days of pregnancy was at 20:00 every night to 8:00 in the morning, and the experimental groups set up virtual exposure as control. The background (temperature, humidity, background noise, ventilation, light and so on) was stable. After the exposure was completed, the offspring rats were produced, and the physiological development and behavioral development indexes were observed and measured. The hippocampus tissue was measured by high performance liquid chromatograph (Glu, Gly, GABA, Asp) by high performance liquid chromatograph after three weeks of age and seven water maze. [results]1. development indicators: (1) physiological development indicators: body weight, eye opening time two experimental group and control group is different (P0.05), compared with the control group, the body weight has a downward trend, the eye opening time is prolonged, and other physiological development indexes are not statistically different. (2) the behavioral development index: the two experimental group of the forelimb suspension was compared with the control group. Compared with the control group (P0.05), compared with the control group, the age of the standard days was lower, and the other behavioral development indexes were not statistically different in the.2. water maze experiment: (1) the water maze test of the three week old rats: the 1800MHz electromagnetic wave exposure, the visual platform experiment, the second day experiment group of the male offspring rats prolonged the escape latency (P0.05) and the female offspring experiment. In one day and second days, the escape latency was prolonged (P0.05) compared with the control group, and the escape latency of the experimental group was prolonged (P0.05) in the first day to the fourth day of the male rat experiment, and the experimental group of the female offspring was longer than the control group in the first day to the third day (P0.05).1800MHz and WiFi superimposed radio frequency field storm. Exposure, visual platform experiment, the first day of the female experiment group was longer than the control group (P0.05), the experiment group was concealed, the experiment group of the male and the female rat were all longer than the control group (P0.05) for the first day to fourth days. (2) the water maze experiment of the seven week old rat: under the exposure of 1800MHz electromagnetic wave, the visual platform experiment, In the second day experiment group, the escape latency of the experimental group was longer than that of the control group (P0.05); the hiding platform experiment, the experiment group of the male offspring and the third day experiment group were longer than the control group, the escape latency was prolonged (P0.05). The first day experiment group of the female offspring was shorter than the control group (P0.05); the space exploration experiment, the female offspring were wearing the platform. The experimental group was more than the control group (P0.05), the percentage of the platform quadrant time (%) was higher than the control group (P0.05).1800MHz and WiFi superposition radiofrequency field exposure, concealed platform experiment, the first day of the male offspring experiment, the third day and the fourth day experiment group were shorter than the control group (P0.05), and the fourth day experiment group of the female offspring rats was compared with the control group. The escape latency shortened (P0.05); space exploration experiment, the percentage (%) of the male offspring's platform quadrant time (%) was higher than the control group (P0.05).3. four amino acid neurotransmitters: under the 1800MHz electromagnetic wave exposure, the expression of Asp expression in the hippocampus of the three week old rats in the experimental group was up to up up (P0.05).1800MHz and WiFi Under field exposure, the expression of Asp expression in hippocampus of three week old rats was up regulated compared with the control group (P0.05). The expression of Glu expression in hippocampal tissue of seven week old rats was down regulated compared with the control group (P0.05). The Asp expression of hippocampus tissue in the experimental group was higher than that at the age of seven weeks at the age of three weeks and at the age of seven weeks, (P0.05). [Conclusion] the experimental conditions were under the condition of this experiment. 1. 1800MHz electromagnetic wave and 1800MHz and WiFi superposition radiofrequency field exposure have influence on the weight and eye opening time of the offspring, and the effects on the forelimb suspension need to be further verified by the influence of the.2. 1800MHz electromagnetic wave and the radio frequency field of 1800MHz and WiFi on the spatial learning ability of the three week old rats, and no effect on the spatial memory ability of the offspring of the three week old mice. The influence of.3. 1800MHz electromagnetic wave and 1800MHz and WiFi superposition radiofrequency field on the expression of amino acid neurotransmitter Asp in the hippocampus of three week old rats was further verified by the influence of the.3. 1800MHz electromagnetic wave and the superposition of the radiofrequency field in the hippocampus of the offspring. The latter could reduce the effect of WiFi on the learning and memory of the offspring of the offspring of the offspring of seven week old mice.
【學(xué)位授予單位】：昆明醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R14

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