低場強(qiáng)脈沖電場殺滅水中大腸桿菌的實(shí)驗(yàn)研究
發(fā)布時(shí)間:2018-06-23 05:30
本文選題:高頻 + 低場強(qiáng) ; 參考:《浙江大學(xué)學(xué)報(bào)(工學(xué)版)》2015年04期
【摘要】:采用高頻低場強(qiáng)脈沖電場系統(tǒng)對水中的大腸桿菌進(jìn)行滅菌研究,探索反應(yīng)器結(jié)構(gòu)、電氣參數(shù)及水質(zhì)參數(shù)等對脈沖電場滅菌效率和能量效率的影響.圓筒式反應(yīng)器體積為500mL,平均脈沖場強(qiáng)為3~12kV/cm,脈沖頻率可以高達(dá)20kHz.最佳高壓電極結(jié)構(gòu)為6層均勻分布的直徑為40mm的網(wǎng)電極.脈沖電場的殺菌效率和能量效率隨著脈寬和場強(qiáng)的增大而提高,水的電導(dǎo)率影響波形和單脈沖能量的變化,從而影響殺菌效果,當(dāng)初始細(xì)菌密度降低時(shí)有利于徹底殺光.脈沖頻率對殺菌無明顯影響,但高頻率能夠大幅縮短處理時(shí)間.當(dāng)脈沖電壓為6kV,脈寬為30μs,水的電導(dǎo)率為2.5μS/cm,細(xì)胞初始密度為103~106 cfu/mL時(shí),在消耗能量200J/mL,水溫40℃的前提下,脈沖電場處理后細(xì)菌密度下降了1、2個(gè)對數(shù).當(dāng)細(xì)菌初始密度低于1 250cfu/mL時(shí),細(xì)菌被全部殺光,實(shí)現(xiàn)了非熱低溫滅菌.
[Abstract]:The sterilization of Escherichia coli in water was studied by using a high frequency and low field pulsed electric field system. The effects of reactor structure, electrical parameters and water quality parameters on the efficiency and energy efficiency of pulsed electric field sterilization were explored. The volume of the cylindrical reactor is 500mL, the average pulse field strength is 310kV / cm, and the pulse frequency can be up to 20kHz. The optimum structure of high voltage electrode is 6 layers of uniform distribution of the grid electrode with diameter of 40mm. The bactericidal efficiency and energy efficiency of pulsed electric field increase with the increase of pulse width and field strength. The conductivity of water affects the wave shape and the change of single pulse energy, thus affects the bactericidal effect. When the initial bacterial density decreases, it is advantageous to kill completely. Pulse frequency has no obvious effect on sterilization, but high frequency can significantly shorten the treatment time. When the pulse voltage is 6 kV, the pulse width is 30 渭 s, the water conductivity is 2.5 渭 s / cm, and the initial cell density is 103 渭 s / mL, the bacterial density decreases by 1 and 2 logarithms under the condition of energy consumption of 200 J / mL and water temperature of 40 鈩,
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