本文選題：日糧中銅脅迫 + 吉富羅非魚幼魚　； 參考：《生態(tài)毒理學(xué)報(bào)》2015年02期

【摘要】：吉富羅非魚是我國(guó)南方沿海地區(qū)水產(chǎn)養(yǎng)殖中的主要經(jīng)濟(jì)魚類之一,但近年來(lái),隨著我國(guó)城鎮(zhèn)化和工業(yè)化進(jìn)程的推進(jìn),羅非魚養(yǎng)殖面臨著前所未有的銅富集的挑戰(zhàn)。為探明日糧中銅脅迫對(duì)吉富羅非魚幼魚抗氧化系統(tǒng)和肝臟組織結(jié)構(gòu)的影響,將1 080條羅非魚幼魚暴露于6個(gè)濃度梯度(0、3、30、300、1 000、3 000 mg·kg-1)的高銅日糧中,通過(guò)60 d的暴露試驗(yàn),實(shí)時(shí)測(cè)定羅非魚血清與肝臟抗氧化能力,監(jiān)測(cè)肝臟病理變化。結(jié)果表明,在本試驗(yàn)條件下,羅非魚幼魚血清和肝臟中MDA的含量隨日糧中銅含量的增加和脅迫時(shí)間的延長(zhǎng)顯著升高,而SOD、GSH-PX和Cu Zn-SOD的活性表現(xiàn)出先升高后降低的趨勢(shì);各組間肝臟表現(xiàn)出不同程度的病變,主要是濁腫變性和脂肪變性,且第Ⅲ、Ⅳ、Ⅴ組肝臟病變嚴(yán)重。綜上,日糧中銅脅迫對(duì)吉富羅非魚幼魚的抗氧化機(jī)能有較明顯的抑制作用,長(zhǎng)時(shí)間的暴露能嚴(yán)重?fù)p傷其肝臟的組織結(jié)構(gòu),因此,建議吉富羅非魚幼魚日糧中銅的實(shí)際含量應(yīng)控制在42.36 mg·kg-1以下。
[Abstract]:Gifu tilapia is one of the main economic fishes in aquaculture in the southern coastal areas of China. However, in recent years, with the development of urbanization and industrialization in China, tilapia culture is facing unprecedented challenges of copper enrichment. In order to find out the effect of copper stress on antioxidant system and liver tissue structure of juvenile fishes of tilapia japonica, 1 080 juvenile tilapia larvae were exposed to 6 high copper diets with a concentration gradient of 300,000,000mg / kg ~ (-1). The experiment was carried out for 60 days. The anti-oxidation ability of serum and liver of tilapia was measured in real time, and the pathological changes of liver were monitored. The results showed that the content of MDA in serum and liver of juvenile tilapia increased significantly with the increase of copper content in diet and the prolongation of stress time, while the activities of GSH-PX and Cu Zn-SOD in SODN increased first and then decreased. The liver lesions were mainly turbidomatous degeneration and steatosis among groups, and the liver lesions in group 鈪，

本文編號(hào)：2048130