本文選題：X射線 + 外周血��； 參考：《蘇州大學》2012年碩士論文

【摘要】：目的： 采用單細胞凝膠電泳技術檢測X射線對人離體外周血中有核細胞的DNA的損傷及損傷修復；探討不同劑量的X射線對人離體精液DNA及運動性的損傷；采用單細胞凝膠電泳技術檢測不同劑量X射線對人離體外周血有核細胞DNA及精子DNA的損傷,評估外周血有核細胞及精子在高劑量X射線照射后DNA的損傷程度。 方法：采用正常人的離體外周血,用能量為6MV的X射線分別給予0Gy、2Gy、4Gy、6Gy、8Gy、10Gy的劑量照射,分別在0.5h,lh,2h用單細胞凝膠電泳技術檢測全血中有核細胞的彗星率,彗尾長度和尾部DNA含量。采用正常人離體的精液以能量為6MeV的X射線分別給予0Gy、2Gy、4Gy、6Gy、8Gy、10Gy劑量的照射。照射后lh,2h,4h,6h,8h,10h,12h檢測精液常規(guī),觀察精子活率,精子活力；照射后1h內(nèi)行單細胞凝膠電泳,觀察彗星率,彗尾長度,尾部DNA含量。采用正常人的外周血和采集正常的人精液,以能量為6MV的X射線分別給予0Gy、2Gy、4Gy、6Gy、8Gy、10Gy的劑量照射。照射后1h內(nèi)行單細胞凝膠電泳檢測人離體外周血中有核細胞和人離體精液中精子的彗星率,彗尾長度和尾巴DNA含量。 結果： 1.0Gy的對照組彗星率1%,2Gy時彗星率到達27.10%,4Gy時彗星率達到76.32%,6Gy,8Gy,10Gy時彗星率在90%以上,接近100%。2Gy,4Gy時經(jīng)2h修復彗星率下降具有統(tǒng)計學意義(p0.05)；6Gy、8Gy、10G時彗星率下降無統(tǒng)計學意義(p0.05)。DNA修復在1小時內(nèi)最快,在1h-2h間修復速率較1h內(nèi)修復速率減慢。 2.2Gy、4Gy、6Gy、8Gy、10Gy劑量照射組與0Gy對照組相比精子的活率和精子活力在各時間點沒有統(tǒng)計學差異(P0.05)；2Gy、4Gy、6Gy、8Gy、10Gy劑量照射組的彗星率分別是47.37±8.32%,92.22±9.025%,100%,100%,100%；與0Gy組(2.10±1.50%)相比彗星率明顯升高,具有明顯的統(tǒng)計學差異(P0.05)。 3.0Gy對照組人外周血中有核細胞的彗星率和精液中精子的彗星率分別是1.00±0.10%,2.1±1.5%,有統(tǒng)計學差異(p0.05)；2Gy組人離體外周血中有核細胞的彗星率和精液中精子的彗星率分別是27.10±4.71%，47.37±8.32%,有統(tǒng)計學差異(p0.05)；4Gy組人離體外周血中有核細胞的彗星率和精液中精子的彗星率分別是76.32±10.28%，92.22±9.02%，有統(tǒng)計學差異(p0.05)；6Gy組人離體外周血中有核細胞的彗星率和精液中精子的彗星率分別是94.36±3.24%，100%，有統(tǒng)計學差異(p0.05)；8Gy，10Gy組人離體外周血中有核細胞的彗星率和精液中精子的彗星率都分別是100%，100%，無統(tǒng)計學差異。 結論： 在2Gy以上的X射線照射下全血中有核細胞的DNA均受到不同程度的損傷。6Gy以上DNA損傷接近100%，且修復明顯減慢或消失，推測：6Gy的X射線可能是全血細胞所能承受的極限。精子受到不同劑量的X線照射后，精子活率和精子活力沒有明顯的變化，仍然具有與卵子結合的運動性；精子的DNA受到了嚴重的損傷。在一定劑量范圍內(nèi)（2-6Gy）精子彗星實驗比人離體外周血中單個核細胞彗星實驗能更敏感的反應X射線對人體的損傷。
[Abstract]:Objective: to detect the DNA damage and repair of nucleated cells in human peripheral blood by single cell gel electrophoresis (SCGE), and to explore the damage to human semen DNA and exercise induced by different doses of X ray. Single cell gel electrophoresis (SCGE) was used to detect the DNA damage of human peripheral blood nucleated cells (PBMC) and spermatozoa (spermatozoa) induced by different doses of X-ray. To evaluate the degree of DNA damage of nucleated cells and sperm in peripheral blood after high dose X-ray irradiation. Methods: in vitro peripheral blood of normal people were irradiated with 0 Gy 2 Gy 2 Gy 4 Gy 4 Gy 4 Gy 6 Gy 10 Gy of X ray respectively. Comet rate, tail length and tail DNA content of nucleated cells in whole blood were detected by single cell gel electrophoresis (SCGE) at 2 h after 0.5 h. Normal human semen in vitro was irradiated with X-rays of energy of 6 MeV at a dose of 10 Gy of 0 Gy 2 Gym 4 Gym 6 Gym 8 Gy 10 Gy respectively. The sperm motility and sperm motility were observed by single cell gel electrophoresis within 1 hour after irradiation, and the comet rate, tail length and tail DNA content were observed by single cell gel electrophoresis (SCGE) within 1 hour after irradiation. The peripheral blood of normal people and normal human semen were collected and irradiated with X-rays of energy of 6 MV. The doses of 0 Gy ~ 2 Gy ~ (4) Gy ~ (4) Gy ~ (6) Gy ~ (6) Gy ~ (8) Gy ~ (10 Gy) were irradiated respectively. The comet rate of nucleated cells in human peripheral blood and sperm in human semen were detected by single cell gel electrophoresis within 1 hour after irradiation. Results: the comet rate of 1.0Gy control group reached 27.10104Gy and the comet rate reached 76.326Gy / 8Gy / 10Gy, and the comet rate was more than 90% when the comet rate reached 27.1010Gy and 8Gy / 10Gy, respectively. The reduction of comet rate after 2 h repair was statistically significant when the comet rate was close to 100.2 Gy and 4Gy. There was no statistical significance in the reduction of comet rate at the time of p0.05Gy6 Gy1 / 10G, and the highest rate of DNA repair was found within 1 hour. The repair rate among 1h-2h was slower than that in 1 hour. The sperm motility and sperm motility in the 2.2Gy 4Gy 4Gy 6Gy 10Gy irradiation group compared with the 0Gy control group were not significantly different at each time point. The comet rate of the 10 Gy irradiation group was 47.37 鹵8.3292.22 鹵9.0252510Gy, and was 2.10 鹵1.50Gy). Higher than the comet rate, The comets rate of nucleated cells in peripheral blood and the comet rate of spermatozoa in semen were 1.00 鹵0.1010 and 2.1 鹵1.5, respectively. There was statistical difference in comet rate of nucleated cells in peripheral blood and sperm in semen. The comets rate of human peripheral blood nucleated cells and sperm were 76.32 鹵10.28 鹵92.22 鹵9.02, respectively. There was statistical difference between the two groups (p 0.05Gy) and spermatozoa (P 0.056Gy), respectively. There was statistical difference in the comets rate of nucleated cells in human peripheral blood and spermatozoa in human peripheral blood of the group of p 0.056.Gy. The comet rate of human peripheral blood was significantly higher than that of the control group (p 0.056.Gy), and the rate of sperm was 76.32 鹵10.28 鹵92.22 鹵9.02, respectively. There was a significant difference between the two groups. The comets rate of sperm in the liquid was 94.36 鹵3.24g / 100, respectively. There was statistical difference in the comets rate of nucleated cells in human peripheral blood and sperm in human peripheral blood and semen in vitro, respectively. Conclusion: there is no statistical difference between the comet rate of sperm and that of spermatozoa. Conclusion: the rate of X-ray irradiation above 2 Gy is higher than 2 Gy. DNA of nucleated cells in the whole blood was damaged by different degrees. The DNA damage was close to 100 Gy, and the repair slowed down or disappeared obviously. It is speculated that the X-ray of 6 Gy may be the limit of the whole blood cell. After different doses of X-ray irradiation, sperm motility and sperm motility remained unchanged, and sperm DNA was seriously damaged. The comet assay of sperm in a certain dose range is more sensitive than the comet assay of mononuclear cells in human peripheral blood to respond to the damage caused by X-ray.
【學位授予單位】：蘇州大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R144

【參考文獻】

相關期刊論文 前10條

1 宋博,鄭履康,鄧麗霞,張橋;單細胞凝膠電泳檢測精子DNA損傷方法初探[J];癌變.畸變.突變;2001年02期

2 劉光偉,董麗華,王珍琦,樸春南,趙紅光,周連福,龔守良;低劑量電離輻射對小鼠睪丸生精細胞P53和Bcl-2蛋白表達的影響[J];吉林大學學報(醫(yī)學版);2004年01期

3 武寧;劉永哲;徐瑞明;劉揚;金順子;;X射線全身照射對小鼠脾細胞和腹腔巨噬細胞凋亡的影響[J];吉林大學學報(醫(yī)學版);2006年06期

4 邱志芳,章孝榮,李淼,陶勇,吳李君;生物標記物在DNA氧化損傷及輻射損傷檢測中的應用[J];動物醫(yī)學進展;2005年02期

5 孫淑清，姜杰，，王彬，王獻理;2GyX射線照射誘發(fā)小鼠精子發(fā)生各階段的染色體畸變及顯性致死突變[J];輻射防護;1995年01期

6 劉光偉,劉淑春,呂哲,龔守良;低劑量電離輻射對小鼠睪丸生精細胞凋亡的影響[J];輻射研究與輻射工藝學報;2003年02期

7 徐光翠;鄢s

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