本文選題：西咪替丁 + 輻射　； 參考：《解放軍醫(yī)學(xué)雜志》2015年12期

【摘要】：目的選擇輻射敏感指標(biāo)(骨髓嗜多染紅細(xì)胞微核率)評(píng)價(jià)西咪替丁對(duì)單次低劑量照射小鼠的損傷防護(hù)作用。方法健康雄性C57BL/6小鼠48只,隨機(jī)分為正常對(duì)照組、模型對(duì)照組、200mg/kg WR2721組(陽(yáng)性藥組)及7.5、15、30mg/kg西咪替丁組(n=8)。西咪替丁組于照射前2h腹腔注射給藥1次,陽(yáng)性藥組照射前連續(xù)腹腔注射給藥2d,每日1次。單次照射劑量為0.7Gy,劑量率為5.83m Gy/min。照射后24h檢測(cè)小鼠外周血象,血清和肝臟中的SOD活力與MDA含量,骨髓DNA含量和骨髓嗜多染紅細(xì)胞微核率。結(jié)果與正常對(duì)照組比較,照后24h小鼠外周血白細(xì)胞顯著降低(P0.01),嗜多染紅細(xì)胞微核率顯著升高(P0.01),除15mg/kg西咪替丁組外,其余各組小鼠骨髓DNA含量顯著降低(P0.01,P0.05)。照后24h各組小鼠SOD活性和MDA含量無(wú)明顯變化。與模型對(duì)照組比較,西咪替丁各劑量組外周血白細(xì)胞和骨髓DNA含量均無(wú)明顯變化;7.5mg/kg西咪替丁組嗜多染紅細(xì)胞微核率為0.027‰,明顯低于模型對(duì)照組(P0.01),且劑量減低系數(shù)(DRF)值達(dá)到3.338。結(jié)論西咪替丁對(duì)0.7Gy單次低劑量照射小鼠嗜多染紅細(xì)胞微核有較好的保護(hù)作用。
[Abstract]:Objective to select radiosensitive index (micronucleus rate of polychromatic erythrocyte in bone marrow) to evaluate the protective effect of cimetidine on single low dose irradiated mice. Methods Forty-eight healthy male C57BL/6 mice were randomly divided into normal control group, model control group with 200 mg / kg WR2721 (positive drug group) and 7. 5 mg / kg cimetidine / kg cimetidine group. Cimetidine group was intraperitoneally injected 2 hours before irradiation, and positive drug group was intraperitoneally injected once daily for 2 days before irradiation. The single dose was 0.7Gy and the dose rate was 5.83m Gy / min. SOD activity and MDA content in serum and liver, bone marrow DNA content and micronucleus rate of bone marrow polychromatic erythrocytes were detected 24 hours after irradiation. Results compared with the normal control group, the peripheral white blood cells (PBWs) decreased significantly and the micronucleus rate of polychromatic erythrocytes increased significantly (P 0.01). Except for the 15mg/kg cimetidine group, the content of DNA in the bone marrow of the other groups decreased significantly. There was no significant change in SOD activity and MDA content in mice 24 h after irradiation. Compared with the model control group, the micronucleus rate of polychromatic erythrocytes in the cimetidine group was 0.027 鈥，

本文編號(hào)：1991066