本文選題：運(yùn)動(dòng) + 雌性大鼠　； 參考：《廣州醫(yī)科大學(xué)》2013年碩士論文

【摘要】：研究目的 本實(shí)驗(yàn)通過(guò)給予不同鐵含量飲食以及長(zhǎng)期低強(qiáng)度游泳運(yùn)動(dòng)，研究雌性大鼠血液鐵狀態(tài)及海馬NHI含量的變化和意義。 研究方法 實(shí)驗(yàn)動(dòng)物為兩批，第一批為斷乳SD雌性大鼠60只，隨機(jī)數(shù)字表法均分為飲食低鐵含量組（12mg/kg，SL2）、標(biāo)準(zhǔn)鐵含量組（45mg/kg，SS2）、高鐵含量組（1000mg/kg，SH2），每組均為靜息組。第二批為斷乳雌性SD大鼠108只，分為飲食低鐵含量組（12mg/kg），飲食標(biāo)準(zhǔn)鐵含量組（45mg/kg）和飲食高鐵含量組（1000mg/kg）。每組再分為運(yùn)動(dòng)組和靜息組，即分為六組，分別為低鐵含量飲食+運(yùn)動(dòng)（EL）、低鐵含量飲食+靜息（SL）、標(biāo)準(zhǔn)鐵含量飲食+運(yùn)動(dòng)（ES）、標(biāo)準(zhǔn)鐵含量飲食+靜息（SS）、高鐵含量飲食+運(yùn)動(dòng)（EH）和高鐵含量飲食+靜息（SH），各組用相應(yīng)的鐵含量飼料喂養(yǎng)1個(gè)月后，運(yùn)動(dòng)組開(kāi)始游泳，每周5天，每天1次，，持續(xù)3個(gè)月。靜息組除不進(jìn)行運(yùn)動(dòng)外，其余處理同對(duì)應(yīng)運(yùn)動(dòng)組。運(yùn)動(dòng)期滿(mǎn)后，大鼠空腹24h，在戊巴比妥鈉麻醉下取靜脈血，測(cè)定紅細(xì)胞相關(guān)指標(biāo)，包括血紅蛋白（Hb）、紅細(xì)胞壓積（HCT）、平均紅細(xì)胞體積（MCV）、平均紅細(xì)胞血紅蛋白量（MCH）、紅細(xì)胞分布寬度（RDW）和血清鐵狀態(tài)指標(biāo)，包括血清鐵（SI）、血漿總鐵結(jié)合力（TIBC）并計(jì)算轉(zhuǎn)鐵蛋白飽和度（TS）。取海馬組織液氮速凍，-80°C冰箱保存，測(cè)定海馬NHI。 研究結(jié)果 1.飲食鐵含量和長(zhǎng)期運(yùn)動(dòng)對(duì)雌性大鼠血液鐵狀態(tài)的影響 飲食鐵含量對(duì)Hb、HCT、MCV、MCH、RDW、TIBC、TS（P0.01）和SI（P0.05）均有顯著影響，運(yùn)動(dòng)對(duì)Hb、HCT、MCH、SI和TS均有影響（P0.05）。 與SS組比較，SL組MCV及MCH顯著降低（P0.01），TS降低（P0.05），RDW及TIBC均顯著升高（P0.01）；SH組Hb降低（P0.05），HCT升高（P0.05），MCH、RDW、SI、TIBC和TS均無(wú)差異（P0.05）。 與ES組比較，EL組MCV、MCH、SI、TS均顯著降低（P0.01）， TIBC及RDW均顯著升高（P0.01）；EH組Hb升高（P0.05），HCT、MCV、MCH、RDW、SI、TIBC和TS均無(wú)差異（P0.05）。 與SL組比較，EL組SI、TS顯著降低（P0.01），Hb、HCT、MCV、MCH升高（P0.05），RDW無(wú)差異（P0.05）。 與SS組比較，ES組Hb、HCT、MCV、MCH均升高（P0.05），RDW、SI、TIBC、TS均無(wú)差異（P0.05）。 與SH組比較，Hb、HCT、MCV、MCH、RDW、SI、TIBC和TS均無(wú)差異（P0.05）。 2.飲食鐵含量和長(zhǎng)期運(yùn)動(dòng)對(duì)雌性大鼠海馬鐵狀態(tài)的影響 飲食鐵含量對(duì)海馬NHI有顯著影響（P0.01），運(yùn)動(dòng)對(duì)海馬NHI無(wú)顯著影響（P0.05）。 與ES組比較，EL組NHI顯著降低（P0.01），EH組無(wú)明顯變化（P0.05）；與SS組比較，SL組NHI顯著降低（P0.01），SH組無(wú)明顯變化（P0.05）；與相應(yīng)的靜息組比，EL、ES、EH組均無(wú)明顯變化（P0.05）。 與第一批靜息組比較，EH組NHI升高（P0.05)，ES、EL組無(wú)明顯變化（P0.05)；SH組NHI顯著升高（P0.01)，SS、SL組無(wú)明顯變化（P0.05)。 研究結(jié)論 1.長(zhǎng)期低鐵含量飲食會(huì)導(dǎo)致大鼠營(yíng)養(yǎng)性鐵缺乏。 2.在低鐵含量飲食時(shí)，長(zhǎng)期運(yùn)動(dòng)會(huì)加劇大鼠血清鐵狀態(tài)的降低；在鐵含量飲食足夠時(shí)，長(zhǎng)期運(yùn)動(dòng)會(huì)導(dǎo)致Hb、HCT升高，但血清鐵無(wú)變化；在高鐵含量飲食時(shí)，長(zhǎng)期運(yùn)動(dòng)對(duì)血液鐵狀態(tài)均無(wú)影響。 3.運(yùn)動(dòng)和飲食鐵含量?jī)梢蛩刂g對(duì)雌性大鼠有交互作用，飲食鐵含量對(duì)海馬NHI有影響，低鐵含量飲食組海馬NHI含量降低。在低鐵含量飲食時(shí)，長(zhǎng)期運(yùn)動(dòng)加劇海馬NHI的降低；在飲食鐵含量充足及高鐵含量飲食時(shí)，運(yùn)動(dòng)后海馬NHI無(wú)顯著變化。 4.在低鐵含量飲食和高鐵含量飲食時(shí)，運(yùn)動(dòng)前海馬NHI均明顯降低，運(yùn)動(dòng)后低鐵含量降低，而高鐵含量飲食恢復(fù)正常水平，提示在高鐵含量飲食時(shí)，運(yùn)動(dòng)不對(duì)雌性大鼠海馬NHI造成影響，海馬具有維持鐵代謝的機(jī)制。
[Abstract]:research objective
This experiment was conducted to investigate the changes of blood iron status and hippocampal NHI content in female rats by giving different iron content diet and long-term low intensity swimming exercise.
research method
The experimental animals were two batches and the first batch of 60 female SD rats were weaned. The random digital table method was divided into the diet low iron content group (12mg/kg, SL2), the standard iron content group (45mg/kg, SS2), the high iron content group (1000mg/kg, SH2), each group was resting group. The second batch of female SD rats were divided into the diet low iron content group (12mg/kg), the dietary standard iron. The content group (45mg/kg) and the diet high iron content group (1000mg/kg) were divided into exercise group and resting group, which were divided into six groups: low iron content diet + exercise (EL), low iron content diet + resting (SL), standard iron content diet + exercise (ES), standard iron content diet + resting (SS), high iron content diet + exercise (EH) and high iron content diet + resting (SH) after feeding on the corresponding iron content feed for 1 months, the exercise group began to swim, 5 days a week, 1 times a day, and lasted 3 months. The rest group was treated with the corresponding exercise group except for no exercise. After the expiration of the exercise period, the rats had a fasting 24h, and the venous blood was taken under pentobarbital anesthesia, and the red blood cell related indexes, including hemoglobin (Hb), were measured. Red blood cell pressure (HCT), average red cell volume (MCV), average red blood cell hemoglobin (MCH), red cell distribution width (RDW) and serum iron status index, including serum iron (SI), plasma total iron junction force (TIBC) and calculation of transferrin saturation (TS). The liquid nitrogen freezer in the hippocampus, -80 / C refrigerator and NHI. for the determination of NHI. in the hippocampus
Research results
1. effects of iron content and long-term exercise on iron status in female rats
Dietary iron content has significant effects on Hb, HCT, MCV, MCH, RDW, TIBC, TS (P0.01) and SI (P0.05), and exercise has an effect on Hb, Hb, RDW, and TIBC.
Compared with group SS, MCV and MCH in SL group decreased significantly (P0.01), TS decreased (P0.05), RDW and TIBC increased significantly (P0.01), SH group Hb decreased.
Compared with the ES group, the MCV, MCH, SI and TS in the EL group were significantly decreased (P0.01), TIBC and RDW increased significantly (P0.01).
Compared with group SL, SI and TS in group EL were significantly decreased (P0.01), Hb, HCT, MCV and MCH increased (P0.05), and there was no difference in RDW (RDW).
Compared with group SS, ES, Hb, HCT, MCV and MCH all increased (P0.05), RDW, SI, TIBC, and TS were not different (P0.05).
Compared with group SH, there was no difference in Hb, HCT, MCV, MCH, RDW, SI, TIBC and TS (P0.05).
2. effect of dietary iron content and long-term exercise on hippocampal status in female rats
Dietary iron content had a significant effect on hippocampal NHI (P0.01), and exercise had no significant effect on hippocampal NHI (P0.05).
Compared with group ES, NHI in group EL decreased significantly (P0.01), and there was no significant change in EH group (P0.05). Compared with group SS, SL group NHI decreased significantly (P0.01), SH group had no significant changes (P0.05).
Compared with the first resting group, NHI in EH group increased (P0.05), ES and EL group did not change significantly (P0.05), NHI in SH group increased significantly (P0.01), SS and SL group did not change significantly (P0.01).
research conclusion
1. long term low iron diet can cause nutritional iron deficiency in rats.
2. in the low iron diet, long-term exercise aggravates the reduction of iron status in the serum of rats; when the iron content is sufficient, long term exercise leads to Hb, HCT increase, but no change in serum iron; in the high iron diet, the long-term exercise has no effect on the blood iron status.
3. exercise and dietary iron content of two factors have interaction with the female rats, the dietary iron content has an effect on the hippocampal NHI, and the content of NHI in the hippocampus of the diet group of low iron content decreases. In the low iron content diet, the long-term exercise aggravates the decrease of the hippocampal NHI; in the diet iron content and the high iron content diet, the movement of Houhai horse NHI has no significant change.
4. in the diet of low iron content and high iron content diet, the NHI of Qianhai horse decreased obviously, the content of low iron after exercise decreased, while the diet of high iron content recovered to normal level, suggesting that exercise did not affect the NHI of hippocampus in the high iron content diet, and the hippocampus had the mechanism of maintaining iron metabolism.
【學(xué)位授予單位】：廣州醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類(lèi)號(hào)】：R151.41

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