本文選題：茁霉多糖 + 擴(kuò)大發(fā)酵��； 參考：《甘肅農(nóng)業(yè)大學(xué)》2012年碩士論文

【摘要】：本實(shí)驗(yàn)以出芽短梗霉誘變菌種D1-11為出發(fā)菌，制備茁霉多糖發(fā)酵液，在小試（100ml）發(fā)酵工藝的基礎(chǔ)上，設(shè)計(jì)了5L擴(kuò)大發(fā)酵生產(chǎn)工藝方案，分別研究了接種量和轉(zhuǎn)速對擴(kuò)大發(fā)酵的影響，對比研究了擴(kuò)大發(fā)酵和小試發(fā)酵液在生物量、多糖產(chǎn)量、蛋白含量及色素等各項(xiàng)指標(biāo)上的差異；研究粉末活性炭法，硅藻土法，有機(jī)溶劑法，雙氧水法等對茁霉多糖發(fā)酵液脫色效果，以脫色率和糖損耗率為指標(biāo)，比較幾種脫色方法的單因素實(shí)驗(yàn)結(jié)果，選擇出較適宜的脫色方法，再通過響應(yīng)面設(shè)計(jì)，研究確定該方法的最佳工藝參數(shù)；研究酶-NaCl-醇法，微波-堿水法以及超聲波-鹽水法等三種多糖提取方法，通過單因素及響應(yīng)面數(shù)據(jù)分析得出最優(yōu)提取方法及工藝。本實(shí)驗(yàn)主要研究結(jié)果如下： （1）擴(kuò)大發(fā)酵生產(chǎn)茁霉多糖工藝條件為：裝液量3.5L，接種量12%，初始pH6.5，溫度30℃，發(fā)酵時(shí)間5d，在此工藝下制得的發(fā)酵液中多糖含量達(dá)到3.66g/L。 （2）硅藻土法最大脫色率87.43%，糖損耗率13.94%；有機(jī)溶劑法脫色最大脫色率69.22%，糖損耗率18.46%；H2O2法最大脫色率80.61%，糖損耗率18.24%；根據(jù)響應(yīng)面結(jié)果，粉末活性炭法脫色最優(yōu)工藝參數(shù)為pH6.3，脫色時(shí)間42min，脫色溫度48℃，酶添加量1%，得出最大脫色率為88.58%，幾種脫色方法中，粉末活性炭脫色率最高，糖損耗最小13.59%，簡單快捷，成本低，適合工業(yè)化生產(chǎn)。 （3）酶-NaCl-醇法提取的最佳工藝條件是pH4，酶用量1.5%，提取溫度55℃，NaCl用量1%，得出最大提取率為64.16%；微波輔助法提取的最佳工藝條件是堿水濃度0.5mol/L，，微波功率P40，提取溫度70℃，微波時(shí)間90s，得出最大提取率為78.66%；超聲波輔助法提取的最佳工藝條件是超聲功率40%，超聲時(shí)間10min，提取溫度70℃，鹽水濃度0.6mol/L，得出最大提取率為79.43%；提取率最高的是超聲波輔助鹽水法，其次是微波輔助堿水法，酶-NaCl-醇法最低，根據(jù)響應(yīng)面實(shí)驗(yàn)結(jié)果分析，超聲波-鹽水法提取率最高，綜合考慮，超聲波輔助鹽水提取法最優(yōu)。
[Abstract]:In this experiment, the mutant strain D1-11 was used as the starting strain to prepare the fermentation broth of pullulan polysaccharide. On the basis of the experiment, the 5L expanded fermentation process was designed, and the effects of inoculation amount and rotational speed on the expanded fermentation were studied, respectively. The differences of biomass, polysaccharide yield, protein content and pigment between expanded fermentation and small scale fermentation broth were studied, including powder activated carbon method, diatomite method, organic solvent method, etc. The decolorization effect of hydrogen peroxide method on the fermentation broth of pullulan was studied. The decolorization rate and sugar loss rate were taken as indexes, the results of single factor experiments of several decolorization methods were compared, the more suitable decolorization method was selected, and then the response surface design was adopted. Three extraction methods of polysaccharides, enzyme NaCl-alcohol method, microwave-alkali water method and ultrasonic salt-water method, were studied. The optimum extraction method and process were obtained by single factor and response surface data analysis. The main results of this experiment are as follows: 1) the conditions of expanded fermentation were as follows: the amount of liquid was 3.5L, the inoculation amount was 12g, the initial pH was 6.5, the temperature was 30 鈩

本文編號(hào)：1965114